Bioactivity screening of fiveCentaureaspecies andin vivoanti-inflammatory activity ofC. athoa

Erel, Ş Baykan; Demir, Serdar; Nalbantsoy, Ayşe; Ballar, Petek; Khan, Shabana I.; Yavaşoğlu, Nefise Ülkü Karabay; Karaalp, Canan

doi:10.3109/13880209.2013.868493

Cited by 33 publications

(22 citation statements)

References 21 publications

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“…Similar to our result, Talhouk et al 44 reported that water extract of C. ainetensis inhibited IL-6 production in a dose-dependent manner. In addition, in vivo anti-inflammatory effects of some Centaurea species were reported as well by Erel et al 45 and Koca et al 46 The present study indicates that induction or inhibition of inflammatory cytokines by ethanolic extract of C. solstitialis is cell-type dependent.…”

Section: Discussionsupporting

confidence: 82%

The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines

Alper

Güneş

2019

tjps

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Bitkilerden elde edilen doğal ürünler, kanser dahil çeşitli hastalıkların tedavisinde uzun yıllardır kullanılmaktadır. Centaurea solstitialis subsp. solstitialis'in Türk geleneksel tıbbında yeri olduğu bilinmektedir. Bu araştırma, Muğla ilinden toplanan C. solstitialis'in çiçekli kısımlarından elde edilen etanolik özütün in vitro biyolojik etkilerini belirleyen ilk çalışmadır. Gereç ve Yöntemler: Özütün Daudi, A549 ve HeLa kanser hücrelerine ve normal BEAS-2B hücre hattına karşı sitotoksik etkisi MTT (3-(4,5dimetiltiyazol-2-il)-2,5-dipeniltetrazolyum bromür) testi ile belirlendi. Apoptotik hücre ölümü akış sitometri analizi ve kaspaz-3 aktivite deneyleriyle araştırıldı. Özüt ile muamele edilen hücreler tarafından üretilen anjiyojenik faktör [vasküler endotel büyüme faktörü (VEGF)] salınımı ve sitokinlerden interlökin (IL)-lα, IL-6 ve tümör nekroz faktör (TNF)-α'nın salınımı enzim bağlı immünosorbent deneyi testleriyle ölçüldü. Bulgular: Bitki özütü kullanılan tüm kanser hücre hatlarına karşı sitotoksik etki gösterirken, özüte karşı en duyarlı hücrelerin 63.18 µg/mL and 69.27 µg/mL olan IC 50 değerleri ile sırasıyla HeLa ve Daudi hücreleri olduğu gözlendi. Seçici sitotoksisite HeLa ve normal BEAS-2B hücre hatları arasında tespit edildi. Bitki özütü S ve G2 fazlarında hücre döngüsü arrestine yol açtı. Buna ilave olarak, HeLa ve A549 hücrelerinde apoptotik hücre ölümü kaydedildi. Ayrıca bitki özütü A549 hücrelerinin VEGF salgılamasında anlamlı bir düşüşe yol açarken, A549 ile Daudi hücrelerinin IL-lα, IL-6 ve TNF-α salgılamasında önemli değişime neden oldu. Sonuç: Bu bulgular kanser tedavisi ve sitokin salgısının modülasyonunda gerekli olan doğal ilaçların geliştirilmesi için C. solstitialis'in çiçekli kısımlarının potansiyel bir kaynak olabileceğini göstermektedir. Anahtar kelimeler: Centaurea solstitialis, kanser hücre hatları, anti-kanser, anti-inflamatuar Objectives: Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer. Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This study was the first to determine the in vitro biological effects of ethanolic extract from the flowering parts of C. solstitialis L. subsp. solstitialis collected from Muğla Province. Materials and Methods: The cytotoxic effect was evaluated against Daudi, A549, and HeLa cancer cells and one normal BEAS-2B cell line using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide) assay. Flow cytometric analysis and the caspase-3 activity assay were performed to detect apoptotic cell death. Angiogenic factor [vascular endothelial growth factor (VEGF)] secretion and the release of interleukin (IL)-1α, IL-6, and tumor necrosis factor (TNF)-α by cells treated with the extract were measured using enzyme-linked immunosorbent assay. Results: The extract exhibited cytotoxic effects against all the cancer cell lines used but HeLa and Daudi were the most sensitive cells, with IC 50 values of 63.18 µg/mL and 69.27 µg/mL, respectively. Selective...

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Section: Discussionsupporting

confidence: 82%

The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines

Alper

Güneş

2019

tjps

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“…Many Centaurea species are used traditionally for medicinal purposes, including Centaurea pulchella, Centaurea drabifolia, Centaurea triumfettii, Centaurea depressa, and Centaurea virgate. Various biological activities of the members of the genus Centaurea L. have been reported previously, such as cytogenetic (Radic et al, 2005), antiinflammatory (Garbacki et al, 1999;Erel et al, 2011Erel et al, , 2014, anticancer , and cytotoxic activities (Seghiri et al, 2009;Ahmed and Kamel, 2014). The chemical composition of the members of this genus was also reported in other studies (Dural et al, 2003;Beltagy, 2015).…”

Section: Introductionmentioning

confidence: 69%

Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line

Yırtıcı¹,

Göğer²,

Sarimahmut³

et al. 2017

Turk J Biol

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“…In comparison with a previous study used the ultrasound assisted extraction and conducted in Turkey, the extraction yield of C. iberica using different solvents (chloroform, methanol, n-Hexane) was (1.46%, 7.04%, 1.14%), respectively. The difference in yield can be explained by different environmental factors (Erel et al, 2014). In this study, the ultrasound assisted extraction technique was used.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis, Total Phenolic Content, Hemolytic and Antihemolytic Activities of Centaurea iberica (Asteraceae)

Joujeh¹

2020

SJKFU

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Several plants contain chemical substances that have various biological activities that might cause harmful effects to cellular membranes. This work deals with Centaurea iberica (Iberian starthistle) plant. The objective of this study is to identify the main chemical groups in the methanolic extract 80% of the plant, determine its total phenolic content, evaluate its toxic effect on human erythrocytes in addition to assessing its activity in protecting normal and glucose-6-phosphate dehydrogenase (G6PD) enzyme-deficient human erythrocytes membranes against oxidative hemolysis. Phytochemical analysis revealed that C. iberica extract contains several bioactive compounds. The results also showed that the plant extract contained good level of total phenolic compounds. The extract exhibited low hemolytic effect. It also showed excellent activity in the protection of normal and G6PDdeficient human erythrocytes against oxidative hemolysis.

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Bioactivity screening of fiveCentaureaspecies andin vivoanti-inflammatory activity ofC. athoa

Abstract: C. athoa is suggested to be a potential source of lead compounds for inflammatory diseases due to the significant in vitro and in vivo anti-inflammatory results.

Cited by 33 publications

References 21 publications

The Anticancer and Anti-inflammatory Effects of <i>Centaurea solstitialis</i> Extract on Human Cancer Cell Lines

The Anticancer and Anti-inflammatory Effects of <i>Centaurea solstitialis</i> Extract on Human Cancer Cell Lines

Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line

Phytochemical Analysis, Total Phenolic Content, Hemolytic and Antihemolytic Activities of Centaurea iberica (Asteraceae)

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