Bioactive Nano-Hydroxyapatite Doped Electrospun PVA-Chitosan Composite Nanofibers for Bone Tissue Engineering Applications

Satpathy, Aishwarya; Pal, Aniruddha; Sengupta, Somoshree; Das, Ankita; Hasan, Md. Mahfujul; Ratha, Itishree; Barui, Ananya; Bodhak, Subhadip

doi:10.1007/s41745-019-00118-8

Cited by 36 publications

(14 citation statements)

References 46 publications

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“…The increase in hydrophobicity can be proven regarding the increase in the contact angles of the heat-treated samples, which in our study changed around 25–53° due to different amounts of cross-linker addition; Perves et al found a larger range of this change: 14.68–64.74° [ 41 ]. As was proved by Sathypathy et al, in the case of other ratios of chitosan to PVA, in special cases, the contact angle can even decrease (as in the case of our I 2 sample) because of the availability of the OH group in the saccharide moieties in chitosan [ 44 ]; however, this does not mean that the sample’s solubility in water would increase. Cui et al, in 2017, showed that two parallel processes can take place: the increase in chitosan content decreased the contact angles, but the cross-linking with glutaraldehyde increased the hydrophobicity of the net [ 26 ].…”

Section: Discussionsupporting

confidence: 51%

“…The swelling behavior of the nanofibrous net also plays an important role in cellular adhesion and proliferation, as it influences the fluid intake capacity of the scaffold. Satpathy et al found a swelling ratio (%) of 130.18% for PVA, and 142.42% for PVA/chitosan [ 44 ]. In our study, with a higher chitosan amount, we found ~3–5 times higher swell ratio values.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of the PVA/Chitosan/Citric Acid Ratio on the Hydrophilicity of Electrospun Nanofiber Meshes

et al. 2021

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In this study, scaffolds were prepared via an electrospinning method for application in oral cavities. The hydrophilicity of the fiber mesh is of paramount importance, as it promotes cell spreading; however, the most commonly used polyvinyl alcohol (PVA) and other hydrophilic fiber meshes immediately disintegrate in aqueous media. In contrast, the excessive hydrophobicity of the scaffolds already inhibits cells adhesion on the surface. Therefore, the hydrophilicity of the fiber meshes needed to be optimized. Scaffolds with different polyvinyl alcohol (PVA)/chitosan/citric acid ratios were prepared. The addition of chitosan and the heat initiated cross-linkage of the polymers via citric acid enhanced the scaffolds’ hydrophobicity. The optimization of this property could be followed by contact angle measurements, and the increased number of cross-linkages were also supported by IR spectroscopy results. The fibers’ physical parameters were monitored via low-vacuum scanning electron microscopy (SEM) and atomic force microscopy (AFM). As biocompatibility is essential for dental applications, Alamar Blue assay was used to prove that meshes do not have any negative effects on dental pulp stem cells. Our results showed that the optimization of the fiber nets was successful, as they will not disintegrate in intraoral cavities during dental applications.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

The Effect of the PVA/Chitosan/Citric Acid Ratio on the Hydrophilicity of Electrospun Nanofiber Meshes

et al. 2021

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“…The peak 1034 cm −1 is tetrahedral PO 4 3− (ν3) mode and peaks 605 and 566 cm −1 are from PO 4 3− (ν3) mode. [ 54,55 ] The PCL–PVA/HA composite core‐sheath fibers consist of all major peaks of the individual materials used to manufacture them confirming the core‐sheath fiber formation and successful incorporation of HA particles on the surface of the fibers.…”

Section: Resultsmentioning

confidence: 85%

Co‐Axial Gyro‐Spinning of PCL/PVA/HA Core‐Sheath Fibrous Scaffolds for Bone Tissue Engineering

Muthusubramanian

Bayram

Gultekinoglu

et al. 2021

Macromolecular Bioscience

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The present study aspires towards fabricating core‐sheath fibrous scaffolds by state‐of‐the‐art pressurized gyration for bone tissue engineering applications. The core‐sheath fibers comprising dual‐phase poly‐ε‐caprolactone (PCL) core and polyvinyl alcohol (PVA) sheath are fabricated using a novel “co‐axial” pressurized gyration method. Hydroxyapatite (HA) nanocrystals are embedded in the sheath of the fabricated scaffolds to improve the performance for application as a bone tissue regeneration material. The diameter of the fabricated fiber is 3.97 ± 1.31 µm for PCL‐PVA/3%HA while pure PCL–PVA with no HA loading gives 3.03 ± 0.45 µm. Bead‐free fiber morphology is ascertained for all sample groups. The chemistry, water contact angle and swelling behavior measurements of the fabricated core‐sheath fibrous scaffolds indicate the suitability of the structures in cellular activities. Saos‐2 bone osteosarcoma cells are employed to determine the biocompatibility of the scaffolds, wherein none of the scaffolds possess any cytotoxicity effect, while cell proliferation of 94% is obtained for PCL–PVA/5%HA fibers. The alkaline phosphatase activity results suggest the osteogenic activities on the scaffolds begin earlier than day 7. Overall, adaptations of co‐axial pressurized gyration provides the flexibility to embed or encapsulate bioactive substances in core‐sheath fiber assemblies and is a promising strategy for bone healing.

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“…The peak 1034 cm −1 is tetrahedral PO 4 3− (𝜈3) mode and peaks 605 and 566 cm −1 are from PO 4 3− (𝜈3) mode. [54,55] The PCL-PVA/HA composite core-sheath fibers consist of all major peaks of the individual materials used to manufacture them confirming the core-sheath fiber formation and successful incorporation of HA particles on the surface of the fibers.…”

Section: Ftir Analysismentioning

confidence: 78%

Co‐Axial Gyro‐Spinning of PCL/PVA/HA Core‐Sheath Fibrous Scaffolds for Bone Tissue Engineering

Muthusubramanian¹,

Bayram²,

Gultekinoglu³

et al. 2021

Macromolecular Bioscience

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The present study aspires towards fabricating core-sheath fibrous scaffolds by state-of-the-art pressurized gyration for bone tissue engineering applications. The core-sheath fibers comprising dual-phase poly-𝝐-caprolactone (PCL) core and polyvinyl alcohol (PVA) sheath are fabricated using a novel "co-axial" pressurized gyration method. Hydroxyapatite (HA) nanocrystals are embedded in the sheath of the fabricated scaffolds to improve the performance for application as a bone tissue regeneration material. The diameter of the fabricated fiber is 3.97 ± 1.31 µm for PCL-PVA/3%HA while pure PCL-PVA with no HA loading gives 3.03 ± 0.45 µm. Bead-free fiber morphology is ascertained for all sample groups. The chemistry, water contact angle and swelling behavior measurements of the fabricated core-sheath fibrous scaffolds indicate the suitability of the structures in cellular activities. Saos-2 bone osteosarcoma cells are employed to determine the biocompatibility of the scaffolds, wherein none of the scaffolds possess any cytotoxicity effect, while cell proliferation of 94% is obtained for PCL-PVA/5%HA fibers. The alkaline phosphatase activity results suggest the osteogenic activities on the scaffolds begin earlier than day 7. Overall, adaptations of co-axial pressurized gyration provides the flexibility to embed or encapsulate bioactive substances in core-sheath fiber assemblies and is a promising strategy for bone healing.

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Bioactive Nano-Hydroxyapatite Doped Electrospun PVA-Chitosan Composite Nanofibers for Bone Tissue Engineering Applications

Cited by 36 publications

References 46 publications

The Effect of the PVA/Chitosan/Citric Acid Ratio on the Hydrophilicity of Electrospun Nanofiber Meshes

The Effect of the PVA/Chitosan/Citric Acid Ratio on the Hydrophilicity of Electrospun Nanofiber Meshes

Co‐Axial Gyro‐Spinning of PCL/PVA/HA Core‐Sheath Fibrous Scaffolds for Bone Tissue Engineering

Co‐Axial Gyro‐Spinning of PCL/PVA/HA Core‐Sheath Fibrous Scaffolds for Bone Tissue Engineering

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