Bioactive compounds of marine dinoflagellate isolates from western Greenland and their phylogenetic association within the genus Alexandrium

Tillmann, Urban; Krock, Bernd; Alpermann, Tilman J.; Cembella, Allan

doi:10.1016/j.hal.2015.11.004

Cited by 20 publications

(19 citation statements)

References 70 publications

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“…9 and Table S9), but these assignments did not apparently correlate well with toxin composition or concentration in plankton. At Station 516, 373 OTUs were assigned to species of Alexandrium, whose presence was also confirmed by microscopy (results not shown, see Tillmann et al, 2016). To differentiate toxic and non-toxic species of Alexandrium, qPCR assays were applied for three stations (515, 516 and 517).…”

Section: Association Of Toxin Derivatives With Potential Hab Speciesmentioning

confidence: 84%

Molecular diversity patterns among various phytoplankton size-fractions in West Greenland in late summer

Elferink

Neuhaus

Wohlrab

et al. 2017

Deep Sea Research Part I: Oceanographic Research Papers

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Arctic regions have experienced pronounced biological and biophysical transformations as a result of global change processes over the last several decades. Current hypotheses propose an elevated impact of those environmental changes on the biodiversity, community composition and metabolic processes of species. The effects on ecosystem function and services, particularly when invasive or toxigenic harmful species become dominant, can be expressed over a wide range of temporal and spatial scales in plankton communities. Our study focused on the comparison of molecular biodiversity of three size-fractions (micro-, nano-, picoplankton) in the coastal pelagic zone of West Greenland and their association with environmental parameters. Molecular diversity was assessed via parallel amplicon sequencing the 28S rRNA hypervariable D1/D2 region. We showed that biodiversity distribution within the area of Uummannaq Fjord, Vaigat Strait and Disko Bay differed markedly within and among size-fractions. In general, we observed a higher diversity within the picoplankton size fraction compared to the nano-and microplankton. In multidimensional scaling analysis, community composition of all three size fractions correlated with cell size, silicate and phosphate, chlorophyll a (chl a) and dinophysistoxin (DTX). Individually, each size fraction community composition also correlated with other different environmental parameters, i.e. temperature and nitrate. We observed a more homogeneous community of the picoplankton across all stations compared to the larger size classes, despite different prevailing environmental conditions of the sampling areas. This suggests that habitat niche occupation for larger-celled species may lead to higher functional trait plasticity expressed as an enhanced range of phenotypes, whereas smaller organisms may compensate for lower potential plasticity with higher diversity. The presence of recently identified toxigenic harmful algal bloom (HAB) species (such as Alexandrium fundyense and A. ostenfeldii) in the area points out the potential risk for this vulnerable ecosystem in a changing world.

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Section: Association Of Toxin Derivatives With Potential Hab Speciesmentioning

confidence: 84%

Molecular diversity patterns among various phytoplankton size-fractions in West Greenland in late summer

Elferink

Neuhaus

Wohlrab

et al. 2017

Deep Sea Research Part I: Oceanographic Research Papers

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“…Instead, their growth responses to temperature were similar to those of temperate isolates, with the maximum growth rate for all isolates found between 16–18 °C. These data will be published separately, along with a detailed analysis of the toxin contents of these isolates (Tillmann et al 2016; D.M. Anderson unpub.…”

Section: Discussionmentioning

confidence: 99%

“…cultures (Supplementary Table S1), either from single cell isolations from plankton samples (Tillmann et al 2016), or from germinated cysts. Additionally, isolates were established from cysts in sediments collected from the Chukchi Sea, which were kindly provided by Dr. Haifeng Gu (Third Institute of Oceanography, Xiamen, P.R.…”

Section: Methodsmentioning

confidence: 99%

“…Cultures were initially maintained at 10 °C, but were subsequently maintained at 15 °C due to improved growth at the higher temperature. Species designations of isolates were determined by sequencing the highly variable D1–D2 domains of the large subunit ribosomal RNA gene (LSU rRNA) (Tillmann et al 2016; D.M. Anderson unpub.…”

Section: Methodsmentioning

confidence: 99%

“…In waters east of North America and north of Europe, A. fundyense has been observed in plankton samples from the Labrador, Greenland, and Norwegian Seas (Scholin 1998, Okolodkov 2005, Baggesen et al 2012, Tillmann et al 2016), and in the Northwest Passage in the Canadian archipelago (M. Levasseur pers. comm.).…”

Section: Introductionmentioning

confidence: 99%

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Distribution of Alexandrium fundyense (Dinophyceae) cysts in Greenland and Iceland, with an emphasis on viability and growth in the Arctic

Richlen¹,

Holinde²,

Tillmann³

et al. 2016

Mar. Ecol. Prog. Ser.

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The bloom-forming dinoflagellate Alexandrium fundyense has been extensively studied due its toxin-producing capabilities and consequent impacts to human health and economies. This study investigated the prevalence of resting cysts of A. fundyense in western Greenland and Iceland to assess the historical presence and magnitude of bloom populations in the region, and to characterize environmental conditions during summer, when bloom development may occur. Analysis of sediments collected from these locations showed that Alexandrium cysts were present at low to moderate densities in most areas surveyed, with highest densities observed in western Iceland. Additionally, laboratory experiments were conducted on clonal cultures established from isolated cysts or vegetative cells from Greenland, Iceland, and the Chukchi Sea (near Alaska) to examine the effects of photoperiod interval and irradiance levels on growth. Growth rates in response to the experimental treatments varied among isolates, but were generally highest under conditions that included both the shortest photoperiod interval (16h:8h light:dark) and higher irradiance levels (~146–366 μmol photons m−2 s−1), followed by growth under an extended photoperiod interval and low irradiance level (~37 μmol photons m−2 s−1). Based on field and laboratory data, we hypothesize that blooms in Greenland are primarily derived from advected Alexandrium populations, as low bottom temperatures and limited light availability would likely preclude in situ bloom development. In contrast, the bays and fjords in Iceland may provide more favorable habitat for germling cell survival and growth, and therefore may support indigenous, self-seeding blooms.

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Effects of light intensity and temperature on growth and ingestion rates of the mixotrophic dinoflagellate Alexandrium pohangense

Lim

Jeong

et al. 2019

Mar Biol

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Bioactive compounds of marine dinoflagellate isolates from western Greenland and their phylogenetic association within the genus Alexandrium

Cited by 20 publications

References 70 publications

Molecular diversity patterns among various phytoplankton size-fractions in West Greenland in late summer

Molecular diversity patterns among various phytoplankton size-fractions in West Greenland in late summer

Distribution of Alexandrium fundyense (Dinophyceae) cysts in Greenland and Iceland, with an emphasis on viability and growth in the Arctic

Effects of light intensity and temperature on growth and ingestion rates of the mixotrophic dinoflagellate Alexandrium pohangense

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