Bio-synthesis and screening of nutrients for lovastatin by Monascus sp. under solid-state fermentation

Dikshit, Rashmi; Tallapragada, Padmavathi

doi:10.1007/s13197-014-1678-y

Cited by 22 publications

(15 citation statements)

References 19 publications

(14 reference statements)

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“…Dikshit and Tallapragada [55] have also showed the production of lovastatin by Monascus sp. was highly influenced by MgSO 4 out of the screened variables.…”

Section: Resultsmentioning

confidence: 96%

Cellular Localization and Production of Lovastatin from Monascus purpureus

Seenivasan¹,

Venkatesan²,

Tapobrata³

2018

pharmaceutical-sciences

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Seenivasan, et al.: Studies on Biosynthesis of LovastatinLovastatin is the first FDA-approved antihypercholesterolemic drug for the treatment of cardiovascular diseases. Monascus purpureus is one of the safest molds for the production of lovastatin that has been used in the preparation of Chinese medicine Red-yeast rice. This investigation dealt with the effect of both static and dynamic culture conditions on the morphological change and localization of lovastatin in Monascus purpureus. In dynamic culture condition, pellet morphology was observed and the maximum intra-and extra-cellular components of lovastatin including both β-hydroxy acid and lactone forms were 1043.45 and 207.94 μg/l, respectively. Filamentous (mat) form of morphology was observed in the static culture condition and the intra-and extracellular concentration of lovastatin were 677.9 and 789.2 μg/l, respectively. Taguchi's L 12 (11 2 ) orthogonal arrays was employed to find the optimal conditions for the submerged production of lovastatin and for the growth of Monascus purpureus. Three physical and five chemical variables were considered in the current experimental study. The maximum production of lovastatin was observed to be 3.66 mg/l. Among the chemical parameters, MnSO 4 and MgSO 4 were the most significant parameters for the production of lovastatin. Physical parameters, viz., agitation rate and temperature, were also equally significant for the production of lovastatin. Ammonium chloride was the most significant parameter among the variables studied for the growth of Monascus purpureus, followed by glucose and phosphorous sources (KH 2 PO 4 ). Statistical analysis showed that those parameters were significant with more than 99 % confidence (p<0.01).

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“…Dikshit and Tallapragada [55] have also showed the production of lovastatin by Monascus sp. was highly influenced by MgSO 4 out of the screened variables.…”

Section: Resultsmentioning

confidence: 96%

Cellular Localization and Production of Lovastatin from Monascus purpureus

Seenivasan¹,

Venkatesan²,

Tapobrata³

2018

pharmaceutical-sciences

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“…purpureus that the adjustment, logarithmic, and stabilization phases were 2, 5, and 12 days, respectively. Monacolin K biosynthesis [50,51] was maximal at 8 days. Monacolin K was extracted from five fractions of M .…”

Section: Resultsmentioning

confidence: 99%

De Novo RNA Sequencing and Transcriptome Analysis of Monascus purpureus and Analysis of Key Genes Involved in Monacolin K Biosynthesis

Zhang

Liang²,

Yang³

et al. 2017

PLoS ONE

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Monascus purpureus is an important medicinal and edible microbial resource. To facilitate biological, biochemical, and molecular research on medicinal components of M. purpureus, we investigated the M. purpureus transcriptome by RNA sequencing (RNA-seq). An RNA-seq library was created using RNA extracted from a mixed sample of M. purpureus expressing different levels of monacolin K output. In total 29,713 unigenes were assembled from more than 60 million high-quality short reads. A BLAST search revealed hits for 21,331 unigenes in at least one of the protein or nucleotide databases used in this study. The 22,365 unigenes were categorized into 48 functional groups based on Gene Ontology classification. Owing to the economic and medicinal importance of M. purpureus, most studies on this organism have focused on the pharmacological activity of chemical components and the molecular function of genes involved in their biogenesis. In this study, we performed quantitative real-time PCR to detect the expression of genes related to monacolin K (mokA-mokI) at different phases (2, 5, 8, and 12 days) of M. purpureus M1 and M1-36. Our study found that mokF modulates monacolin K biogenesis in M. purpureus. Nine genes were suggested to be associated with the monacolin K biosynthesis. Studies on these genes could provide useful information on secondary metabolic processes in M. purpureus. These results indicate a detailed resource through genetic engineering of monacolin K biosynthesis in M. purpureus and related species.

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“…The relative effects of two variables on response were investigated from three dimensional plots. The statistical parameters were analyzed using analysis of variance (ANOVA) with a significance level of p < 0.05 (Dikshit & Tallapragada 2015).…”

Section: Rsm Experimental Designmentioning

confidence: 99%

“…Studies seeking to develop and enhance lovastatin production have mainly focused on finding new strains of source fungi and the optimization of fermentation parameters such as temperature, humidity, time and aeration (Dikshit & Tallapragada 2015). In the case of secondary metabolite production, the type of inoculum, either from fungi or hyphae, is also very important (Samiee et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Suwannarat¹,

Iewkittayakorn²,

Sukpondma³

et al. 2019

JSM

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The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the objective of this study was to optimize lovastatin production from this strain using a central composite design developed through response surface methodology (RSM). The inoculum was prepared from mycelium or spore cultured in static liquid and shaking systems with potato dextrose broth (PDB) as the medium. The best inoculum preparation and cultivation systems were selected to study the optimization of the four fermentation parameters of medium concentration (X-5X), temperature (25-37°C), culture medium to flask volume ratio (2.5-12.5) and time (7-35 days). The best inoculum preparation was the mycelium method and the best cultivation system was the static liquid system. In the optimization procedure, a yield of 1315.69 mg/L lovastatin was obtained at a PDB concentration of 5X, a temperature of 25°C, a medium to flask volume ratio of 1:2.5 and a time of 21 days. In a model employing the optimized parameters, temperature was found to have the most effect on lovastatin production, which will decrease if temperature increases above 25°C. Based on this study, A. sclerotiorum PSU-RSPG178 can be a useful strain for producing lovastatin and RSM as an optimization technique not only reduced the number of experiments but also resulted in an optimized lovastatin yield. ABSTRAK Kesan lovastatin pada sintesis kolesterol telah diketahui. Walaupun lovastatin dihasilkan daripada beberapa spesiesAspergillus dan sistem penanaman, hasil bagi lovastatin daripada Aspergillus sclerotiorum PSU-RSPG178 dalam sistem cecair statik masih belum ditentukan. Oleh itu, objektif kajian ini adalah untuk mengoptimumkan pengeluaran lovastatin daripada strain ini menggunakan reka bentuk komposit pusat yang dibangunkan melalui kaedah gerak balas permukaan (RSM). Inokulum disediakan daripada miselium atau spora dikultur dalam cecair statik dan sistem goncangan dengan kaldu kentang dektrosa (PDB) sebagai medium. Sistem penyediaan dan penanaman inokulum terbaik telah dipilih untuk kajian pengoptimuman empat parameter penapaian kepekatan sederhana (X-5X), suhu (25-37°C), kultur medium kepada nisbah isi padu kelalang (2.5-12.5) dan masa (7-35 hari). Persediaan inokulum terbaik adalah kaedah miselium dan sistem penanaman terbaik adalah sistem cecair statik. Dalam prosedur pengoptimuman, hasil lovastatin sebanyak 1315.69 mg/L telah diperoleh pada kepekatan PDB 5X, suhu 25°C, medium kepada nisbah isi padu kelalang 1:2.5 dan tempoh masa 21 hari. Model yang menggunakan parameter optimum, suhu didapati memberi kesan ketara pada pengeluaran lovastatin, yang akan berkurang jika suhu meningkat melebihi 25°C. Berdasarkan kajian ini, A. sclerotiorum PSU-RSPG178 boleh menjadi strain yang berguna untuk menghasilkan lovastatin dan RSM sebagai teknik pengoptimuman yang bukan s...

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Bio-synthesis and screening of nutrients for lovastatin by Monascus sp. under solid-state fermentation

Cited by 22 publications

References 19 publications

Cellular Localization and Production of Lovastatin from Monascus purpureus

Cellular Localization and Production of Lovastatin from Monascus purpureus

De Novo RNA Sequencing and Transcriptome Analysis of Monascus purpureus and Analysis of Key Genes Involved in Monacolin K Biosynthesis

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

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