Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Abstract

The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the objective of this study was to optimize lovastatin production from this strain using a central composite design developed through response surface methodology (RSM). The inoculum was prepared from mycelium or spore cultured in static liquid and shaking systems with potato dextrose broth (PDB) as the medium. The best inoculum preparation and cultivation systems were selected to study the optimization of the four fermentation parameters of medium concentration (X-5X), temperature (25-37°C), culture medium to flask volume ratio (2.5-12.5) and time (7-35 days). The best inoculum preparation was the mycelium method and the best cultivation system was the static liquid system. In the optimization procedure, a yield of 1315.69 mg/L lovastatin was obtained at a PDB concentration of 5X, a temperature of 25°C, a medium to flask volume ratio of 1:2.5 and a time of 21 days. In a model employing the optimized parameters, temperature was found to have the most effect on lovastatin production, which will decrease if temperature increases above 25°C. Based on this study, A. sclerotiorum PSU-RSPG178 can be a useful strain for producing lovastatin and RSM as an optimization technique not only reduced the number of experiments but also resulted in an optimized lovastatin yield. ABSTRAK Kesan lovastatin pada sintesis kolesterol telah diketahui. Walaupun lovastatin dihasilkan daripada beberapa spesiesAspergillus dan sistem penanaman, hasil bagi lovastatin daripada Aspergillus sclerotiorum PSU-RSPG178 dalam sistem cecair statik masih belum ditentukan. Oleh itu, objektif kajian ini adalah untuk mengoptimumkan pengeluaran lovastatin daripada strain ini menggunakan reka bentuk komposit pusat yang dibangunkan melalui kaedah gerak balas permukaan (RSM). Inokulum disediakan daripada miselium atau spora dikultur dalam cecair statik dan sistem goncangan dengan kaldu kentang dektrosa (PDB) sebagai medium. Sistem penyediaan dan penanaman inokulum terbaik telah dipilih untuk kajian pengoptimuman empat parameter penapaian kepekatan sederhana (X-5X), suhu (25-37°C), kultur medium kepada nisbah isi padu kelalang (2.5-12.5) dan masa (7-35 hari). Persediaan inokulum terbaik adalah kaedah miselium dan sistem penanaman terbaik adalah sistem cecair statik. Dalam prosedur pengoptimuman, hasil lovastatin sebanyak 1315.69 mg/L telah diperoleh pada kepekatan PDB 5X, suhu 25°C, medium kepada nisbah isi padu kelalang 1:2.5 dan tempoh masa 21 hari. Model yang menggunakan parameter optimum, suhu didapati memberi kesan ketara pada pengeluaran lovastatin, yang akan berkurang jika suhu meningkat melebihi 25°C. Berdasarkan kajian ini, A. sclerotiorum PSU-RSPG178 boleh menjadi strain yang berguna untuk menghasilkan lovastatin dan RSM sebagai teknik pengoptimuman yang bukan s...

Lovastatin Production by Aspergillus sclerotiorum Using Agricultural Waste

“…( 15 ). The mycelial plugs were used since a spore inoculum gave a low yield of lovastatin in a previous study ( 14 ).…”

“…Mycelial plugs (0.5 cm×0.5 cm) cut from the periphery of an actively growing colony were used as the inoculum according to the method of Daengrot et al (15). The mycelial plugs were used since a spore inoculum gave a low yield of lovastatin in a previous study (14).…”

“…sclerotiorum PSU-RSPG 178, which was isolated from a soil sample collected from the Plant Genetic Conservation Project under the Royal Initiation of Her Royal Highness Princess Maha Chakri Sirindhorn at Ratchaprapa Dam in Suratthani Province, Thailand. This strain produced a high yield of lovastatin, 1316 mg / L, in nutrient broth medium ( 14 ) and therefore seems to have good potential for lovastatin production.…”

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