Binding-regulated click ligation for selective detection of proteins

Cao, Ya; Han, Peng; Wang, Zhuxin; Chen, Weiwei; Shu, Yongqian; Xiang, Yang

doi:10.1016/j.bios.2015.11.034

Cited by 15 publications

(3 citation statements)

References 36 publications

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“…Next, we will functionalize the upper electrode of the sensor by coating it with selective materials to speci cally detect the concentrations of various ions, proteins, glucose, oxygen, and so on. [35][36][37][38][39][40] After the functionalized sensor is combined with the marker, the permittivity and conductivity above the sensor will change. Therefore, the scene in which many microrobots patrol the body under external control are navigated by MRI and actively nd abnormal parts according to the sensing signal for in-situ diagnosis and even treatment will be realized in the near future.…”

Section: Resultsmentioning

confidence: 99%

Self-sensing Intelligent Microrobots for Non-Invasive and Wireless Monitoring Systems

Zhao

Wang

et al. 2023

Preprint

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Microrobots present great potential and wide applications in in-situ treatment and attract tremendous attention due to their small size and flexible movement. However, functional modification for microrobots became more important for their interaction with the environment, except for precise motion control. Here, we design a novel artificial intelligence (AI) microrobot, which can respond to changes in the external environment without onboard energy supplying and transmit signals wirelessly in real time. The AI microrobot can cooperate with external electromagnetic imaging equipment and enhance the local radiofrequency (RF) magnetic field to achieve a large penetration sensing depth and a high spatial resolution. The working ranges are determined by the structure of the sensor circuit and the corresponding enhancement effect can be modulated by the conductivity and permittivity of the surrounding environment, reaching ~ 560 times at most. Under the control of an external magnetic field, the magnetic tail can actuate the microrobotic agent to move accurately, with great potential to realize in-situ monitoring in different places in a human body in an almost noninvasive fashion, especially around potential diseases, which is of great significance for early disease discovery and accurate diagnosis. In addition, the compatible fabrication process provides an approach to swarms of functional microrobots. The findings highlight the feasibility of the self-sensing AI microrobot for the development of in-situ diagnosis or even treatment according to the sensing signals.

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Section: Resultsmentioning

confidence: 99%

Self-sensing Intelligent Microrobots for Non-Invasive and Wireless Monitoring Systems

Zhao

Wang

et al. 2023

Preprint

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“…372 Click chemistry was also used to detect the binding of small a guest molecule to a protein. 373 To a pair of oligonucleotides, an azide and a ligand are bound respectively. When there exists in the solution an enzyme (or relevant protein) which binds to the ligand, the azide is placed in highly crowded environments due to the protein-ligand conjugate formation.…”

Section: Determination Of Dna Nanostructures In Solutionsmentioning

confidence: 99%

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

Komiyama

Yoshimoto

Sisido

et al. 2017

Bulletin of the Chemical Society of Japan

275

131

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In this review, we introduce two kinds of bio-related nanoarchitectonics, DNA nanoarchitectonics and cellmacromolecular nanoarchitectonics, both of which are basically controlled by chemical strategies. The former DNA-based approach would represent the precise nature of the nanoarchitectonics based on the strict or "digital" molecular recognition between nucleic bases. This part includes functionalization of single DNAs by chemical means, modification of the main-chain or side-chain bases to achieve stronger DNA binding, DNA aptamers and DNAzymes. It also includes programmable assemblies of DNAs (DNA Origami) and their applications for delivery of drugs to target sites in vivo, sensing in vivo, and selective labeling of biomaterials in cells and in animals. In contrast to the digital molecular recognition between nucleic bases, cell membrane assemblies and their interaction with macromolecules are achieved through rather generic and "analog" interactions such as hydrophobic effects and electrostatic forces. This cell-macromolecular nanoarchitectonics is discussed in the latter part of this review. This part includes bottom-up and top-down approaches for constructing highly organized cell-architectures with macromolecules, for regulating cell adhesion pattern and their functions in twodimension, for generating three-dimensional cell architectures on micro-patterned surfaces, and for building synthetic/natural macromolecular modified hybrid biointerfaces.

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“…[11][12][13] To date, such a concept has proven to be powerful for generating artificial switches, and has recently been explored for potential functions in the biosensing area. [14][15][16][17] Herein, by rationally combining the aptameric regulation of enzyme catalysis with a specific small-molecule ligand-protein binding event that possesses broad analytical application prospects, [18][19][20][21] we propose a new sensing method for the detection of cell-surface proteins in a sensitive and selective manner. Scheme 1 may illustrate the principle of the method, in which the biotin receptor is chosen as a model target due to the following reasons: (1) the biotin receptor exhibits ultrahigh binding affinity toward its small-molecule ligand (biotin);…”

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confidence: 99%

Binding-responsive catalysis of Taq DNA polymerase for the sensitive and selective detection of cell-surface proteins

Wang

Han

et al. 2016

Chem. Commun.

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Binding-regulated click ligation for selective detection of proteins

Cited by 15 publications

References 36 publications

Self-sensing Intelligent Microrobots for Non-Invasive and Wireless Monitoring Systems

Self-sensing Intelligent Microrobots for Non-Invasive and Wireless Monitoring Systems

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

Binding-responsive catalysis of Taq DNA polymerase for the sensitive and selective detection of cell-surface proteins

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