Binding-responsive catalysis of Taq DNA polymerase for the sensitive and selective detection of cell-surface proteins

Wang, Zhuxin; Li, Yifei; Han, Peng; Mao, Xiaoxia; Yin, Yuan; Cao, Ya

doi:10.1039/c6cc04351h

Cited by 7 publications

(1 citation statement)

References 29 publications

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“…Moreover, many efforts have been devoted to the exploration of strategies of integrating enzymes into the aptamer-based nanodevices. For instance, an aptamer-based approach has been proposed to regulate DNA polymerase activity ( 12–15 ) and this approach has also been employed in biosensing to detect target DNA ( 14 ), protein ( 16–18 ), enzymes ( 19 , 20 ), as well as in constructing molecular circuits ( 21 ). However, the canonical aptamer structures in use usually adopt a duplex form, which limits the application of enzyme owing to deficiency of a programmable and controllable regulation of enzyme activity.…”

Section: Introductionmentioning

confidence: 99%

Switching the activity of Taq polymerase using clamp-like triplex aptamer structure

Wang

Chen

et al. 2020

Nucleic Acids Research

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Abstract In nature, allostery is the principal approach for regulating cellular processes and pathways. Inspired by nature, structure-switching aptamer-based nanodevices are widely used in artificial biotechnologies. However, the canonical aptamer structures in the nanodevices usually adopt a duplex form, which limits the flexibility and controllability. Here, a new regulating strategy based on a clamp-like triplex aptamer structure (CLTAS) was proposed for switching DNA polymerase activity via conformational changes. It was demonstrated that the polymerase activity could be regulated by either adjusting structure parameters or dynamic reactions including strand displacement or enzymatic digestion. Compared with the duplex aptamer structure, the CLTAS possesses programmability, excellent affinity and high discrimination efficiency. The CLTAS was successfully applied to distinguish single-base mismatches. The strategy expands the application scope of triplex structures and shows potential in biosensing and programmable nanomachines.

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