Catalytic hairpin assembly-programmed formation of clickable nucleic acids for electrochemical detection of liver cancer related short gene

Wang, Meiwen; Tang, Yingying; Chen, Yaoyao; Cao, Ya; Chen, Guifang

doi:10.1016/j.aca.2018.08.057

Cited by 21 publications

(9 citation statements)

References 40 publications

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“…Ethanolamine was received from Fisher Scientific, (Massachusetts, USA). All the following 15 mers, probe sequence: 5 -COOH-C6-TAGGAAAGGCTGCCA-3 ; complete complementary target sequence: 5 -TGGCAGCCTTTCCTA-3 ; single-base mismatched sequence: 5 -TGGCAGCgTTTCCTA-3 ; three-base mismatched sequence: 5 -TGcCAGgCTTTCgTA-3 ; and noncomplementary sequence: 5 -TAGGAAAGGCTGCCA-3 [14,15] were chemically synthesized from Apical Scientific Oligo (Kuala Lumpur, Malaysia).…”

Section: Methodsmentioning

confidence: 99%

Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface

Song

Yang

Gopinath

2020

Biotech and App Biochem

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A high-performance interdigitated electrode (IDE) biosensing surface was reported here by utilizing self-assembled silica nanoparticle (SiNP). The modified surface was used to evaluate the complementation of hairpin forming region from Mitoxantrone resistance gene 7 (MXR7; liver cancer-related short gene). The conjugated SiNPs on 3-aminopropyl triethoxysilane functionalization were captured with probe sequence on IDE biosensing surface. The physical and chemically modified surface was used to quantify MXR7 and an increment in the current response upon complementation was noticed. Limit of target DNA detection was calculated (1-10 fM) and this label-free detection is at the comparable level to the fluorescent-based sensing. A linear regression was calculated [y = 0.243x − 0.0773; R 2 = 0.9336] and the sensitivity was 1 fM on the linear range of 1 fM to 10 pM. With the strong attachment of capture DNA on IDE through SiNP, the surface clearly discriminates the specificity (complementary) versus nonspecificity (complete-, single-, and triple-mismatched sequences). This detection strategy helps to determine liver cancer progression and the similar strategy can be followed for other gene sequence complementation.

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Section: Methodsmentioning

confidence: 99%

Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface

Song

Yang

Gopinath

2020

Biotech and App Biochem

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“…As we are advancing in genomics research, nucleic acid detection is attracting more and more attention. The development of biosensors for nucleic acid detection has many benefits related to biochemical studies,56 medical diagnosis,57 and therapeutic applications 58. For example, Guo et al58 reported a surface enhanced raman spectroscopy (SERS) nanosensor which can simultaneously detect multiple disease-related nucleic acids for early disease screening, such as prostate carcinoma, and helping doctors make treatment decisions.…”

Section: Applications Of Biosensorsmentioning

confidence: 99%

“…For example, Guo et al58 reported a surface enhanced raman spectroscopy (SERS) nanosensor which can simultaneously detect multiple disease-related nucleic acids for early disease screening, such as prostate carcinoma, and helping doctors make treatment decisions. Wang et al57 developed a catalytic hairpin assembly-based (CHA) electrochemical biosensor for detecting the liver cancer related short gene MXR7. In food research, Xu et al56 reported using a nucleic acid biosensor which combines loop-mediated isothermal amplifications for universal mammalian identification, especially meat adulteration on site.…”

Section: Applications Of Biosensorsmentioning

confidence: 99%

Recent Advances in Biosensors for Nucleic Acid and Exosome Detection

Zhang

Lai

2019

Chonnam Med J

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Biosensors are analytical devices for biomolecule detection that compromise three essential components: recognition moiety, transducer, and signal processor. The sensor converts biomolecule recognition to detectable signals, which has been applied in diverse fields such as clinical monitoring, in vitro diagnostics, food industry etc. Based on signal transduction mechanisms, biosensors can be categorized into three major types: optical biosensors, electrochemical biosensors, and mass-based biosensors. Recently, the need for faster, more sensitive detection of biomolecules has compeled researchers to develop various sensing techniques. In this review, the basic structure and sensing principles of biosensors are introduced. Additionally, the review discusses multiple recent works about nucleic acid and exosome sensing.

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“…A variety of liquid biopsy methods for liver cancer biomarkers have been developed, including electrochemical, [12][13][14][15][16] acoustics, 17 and fluorescence detection. [18][19][20][21][22][23] Although electrochemical detection excels in vastly reducing ambient interferences, it involves complicated procedures and therefore remains difficult to implement clinically.…”

Section: Introductionmentioning

confidence: 99%

An Approach to the Simultaneous Detection of Multiple Biomarkers for the Early Diagnosis of Liver Cancer Using Quantum Dot Nanoprobes

Cheng

2022

Infectious Microbes and Diseases

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Biomarker-based early diagnosis of liver cancer is of high clinical value for reducing the mortality rate. However, it has been challenging to establish early detection methods with a single biomarker such as alpha-fetoprotein (AFP) because of limited diagnostic sensitivity and specificity. Therefore, developing multiplexed biomarker detection assays is crucially important for early diagnosis. Yet, simultaneous detection methods involving three or more biomarkers have been scarce. Here we suggest employing the serological biomarker panel of glypican-3 (GPC3), dickkopf-1 (DKK1), and AFP for liver cancer detection. We present a rapid simultaneous detection approach for the biomarker panel labeled with three fluorescent quantum dot nanoprobes (emission wavelengths at 565 nm, 605 nm, and 655 nm). As a proof-of-concept, simultaneous fluorescence detection of the biomarker panel was demonstrated using mixed reference samples containing human recombinant GPC3, DKK1, and AFP antigens. Our simultaneous detection approach conferred a linear range of 0.625-2.5 ng•mL À1 for the entire biomarker panel, which merits further clinical validation for the simultaneous and accurate determination of the biomarker panel in human serum samples.

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Catalytic hairpin assembly-programmed formation of clickable nucleic acids for electrochemical detection of liver cancer related short gene

Cited by 21 publications

References 40 publications

Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface

Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface

Recent Advances in Biosensors for Nucleic Acid and Exosome Detection

An Approach to the Simultaneous Detection of Multiple Biomarkers for the Early Diagnosis of Liver Cancer Using Quantum Dot Nanoprobes

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