Bifunctional nanomaterials for simultaneously improving cell adhesion and affecting bacterial biofilm formation on silicon-based surfaces

Motealleh, Andisheh; Dorri, Pooya; Czieborowski, Michael; Philipp, Bodo; Kehr, Nermin Seda

doi:10.1088/1748-605x/abd872

Cited by 5 publications

(3 citation statements)

References 59 publications

(68 reference statements)

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“…The combination of violet-blue light treatment and photosensitisers for surface decontamination has the potential for wide use in healthcare applications. The biocompatibility and stability of TiO 2 allows for its incorporation into a range of polymeric materials for medical devices, including commonly used elastomers [22,23] such as polydimethylsiloxane (PDMS). It can be incorporated into materials by a range of different methods [17] that either result in surface coating or bulk doping.…”

Section: Introductionmentioning

confidence: 99%

Metal oxide-doped elastomeric materials for amplifying visible light-based antimicrobial activity

McShea¹,

Kambo²,

Maclean³

et al. 2022

Mater. Res. Express

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Healthcare-associated infection through transmission of pathogenic bacteria poses a huge threat to public health. One of the main transmission routes is via contaminated surfaces, including those of medical devices, and therefore significant efforts are being invested in developing new surface decontamination strategies. This includes visible light-based approaches, which offer improved compatibility with mammalian cells but lower germicidal efficacy with respect to UV-light. This study investigates the potential to enhance the antimicrobial efficacy of 405 nm light for surface decontamination through use of a photocatalytic TiO2-doped elastomer, elastomers being selected due to their wide use in biomaterials. Poly(dimethylsiloxane) (PDMS) was doped with TiO2 nanoparticles and the surface elastomer etched to expose the embedded nanoparticles. As etching results in increased surface roughness, samples with control nanoparticles (SiO2and Fe3O4) were also investigated to decouple the effects of roughness and photoinactivation upon bacterial attachment and inactivation. Characterisation by SEM, AFM and contact angle analysis confirmed that etching produced a rougher (39.3±15.3 vs 5.11±1.29 nm RMS roughness; etched vs unetched TiO2-PDMS), more hydrophobic surface (water contact angle of 120±2.5° vs 110±1.0°; etched TiO2-PDMS vs native PDMS). This surface, rich in exposed photocatalytic TiO2 nanoparticles, allows direct contact between contaminating bacteria and nanoparticles, enabling ROS generation in closer proximity to the bacteria and consequent enhancement of visible light treatment. Incorporating TiO2 into PDMS significantly improved the photoinactivation efficacy (mean bacterial count for light-treated samples normalised to untreated samples of 0.043±0.0081) compared to PDMS alone (0.19±0.036), when seeded with Staphylococcus aureus and exposed to 405 nm, 60 J cm-2 light. However, photoinactivation efficacy was significantly (p<0.001) enhanced by etching the TiO2-PDMS surface (0.015±0.0074), resulting in greater photoinactivation than that obtained for etched (47.0±14.5 nm RMS roughness), non-photocatalytic SiO2-PDMS (0.10±0.093). Results suggest this doping and etching strategy shows significant potential for facilitating decontamination of elastomer-based biomaterials.

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Section: Introductionmentioning

confidence: 99%

Metal oxide-doped elastomeric materials for amplifying visible light-based antimicrobial activity

McShea¹,

Kambo²,

Maclean³

et al. 2022

Mater. Res. Express

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“…Recently, we reported the synthesis of bifunctional periodic mesoporous organosilica (PMO) functionalized with antibacterial and cell-adhesive dexamethasone and poly-D-lysine (PDL) 23 and their incorporation onto glass and polydimethylsiloxane surfaces. 24 The prepared bifunctional PMO and the respective silicon-based surfaces were used to improve cell adhesion and to reduce bacterial bioluminescence (fitness).…”

Section: ■ Introductionmentioning

confidence: 99%

“…In one interesting study, Li et al demonstrated that the antibacterial activity of phenylalanyl-polyethylenimine was activated via cucurbit[8]uril (CB) cross-linking that was mediated by supramolecular host–guest chemistry to reduce and/or control the antibiotic resistance. Recently, we reported the synthesis of bifunctional periodic mesoporous organosilica (PMO) functionalized with antibacterial and cell-adhesive dexamethasone and poly- d -lysine (PDL) and their incorporation onto glass and polydimethylsiloxane surfaces . The prepared bifunctional PMO and the respective silicon-based surfaces were used to improve cell adhesion and to reduce bacterial bioluminescence (fitness).…”

Section: Introductionmentioning

confidence: 99%

Functional Nanomaterials and 3D-Printable Nanocomposite Hydrogels for Enhanced Cell Proliferation and for the Reduction of Bacterial Biofilm Formation

Motealleh

Kart

Czieborowski

et al. 2021

ACS Appl. Mater. Interfaces

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Biomaterial-associated infections are a major cause of biomaterial implant failure. To prevent the initial attachment of bacteria to the implant surface, researchers have investigated various surface modification methods. However, most of these approaches also prevent the attachment, spread, and growth of mammalian cells, resulting in tissue integration failure. Therefore, the success of biomaterial implants requires an optimal balance between tissue integration (cell adhesion to biomaterial implants) and inhibition of bacterial colonization. In this regard, we synthesize bifunctional nanomaterials by functionalizing the pores and outer surfaces of periodic mesoporous organosilica (PMO) with antibacterial tetracycline (Tet) and antibacterial and cell-adhesive bipolymer poly-d-lysine (PDL), respectively. Then, the fabricated TetPMO-PDL nanomaterials are incorporated into alginate-based hydrogels to create injectable and 3D-printable nanocomposite (NC) hydrogels (AlgL-TetPMO-PDL). These bifunctional nanomaterial and 3D-printable NC hydrogel show pH-dependent release of Tet over 7 days. They also enhance the proliferation of eukaryotic cells (fibroblasts). TetPMO-PDL is inactive in reducing Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis biofilms. However, AlgL-TetPMO-PDL shows significant antibiofilm activity against P. aeruginosa. These results suggest that the incorporation of TetPMO-PDL into AlgL may have a synergistic effect on the inhibition of the Gram-negative bacterial (P. aeruginosa) biofilm, while this has no effect on the reduction of the Gram-positive bacterial (S. aureus and E. faecalis) biofilm.

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Antimicrobial Metal and Metal Oxide Nanoparticles in Bone Tissue Repair

Shineh,

Mobaraki,

Afzali

et al. 2024

Biomedical Materials & Devices

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Bifunctional nanomaterials for simultaneously improving cell adhesion and affecting bacterial biofilm formation on silicon-based surfaces

Cited by 5 publications

References 59 publications

Metal oxide-doped elastomeric materials for amplifying visible light-based antimicrobial activity

Metal oxide-doped elastomeric materials for amplifying visible light-based antimicrobial activity

Functional Nanomaterials and 3D-Printable Nanocomposite Hydrogels for Enhanced Cell Proliferation and for the Reduction of Bacterial Biofilm Formation

Antimicrobial Metal and Metal Oxide Nanoparticles in Bone Tissue Repair

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