Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase

Chauhan, Nidhi; Tiwari, Sangeeta; Narayan, Tarun; Jain, Utkarsh

doi:10.1016/j.apsusc.2018.04.056

Cited by 38 publications

(14 citation statements)

References 28 publications

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“…54 In another report, AChE and ChO immobilized on gold electrode modified with platinum nanoparticles and metal organic framework were used for the simultaneous detection of acetylcholine and choline in serum. 55 An even better detection limit was reported for AChE-ChOx cross-linked with glutaraldehyde and bovine nanoparticles (AuNps). On the top of this layer, diallyl dimethylammonium chloride (PDDA) and AChE were coated in a layer-by-layer assembly to optimize the AChE loading.…”

Section: ■ Acetylcholine (Ach)mentioning

confidence: 99%

“…Acetylcholine esterase and choline oxidase were adsorbed on the surface of the GCE/GO-IL electrode and used as the working electrode for the simultaneous detection of ACh and Ch . In another report, AChE and ChO immobilized on gold electrode modified with platinum nanoparticles and metal organic framework were used for the simultaneous detection of acetylcholine and choline in serum . An even better detection limit was reported for AChE-ChOx cross-linked with glutaraldehyde and bovine serum albumin on polypyrrole-polynvinyln sulfonate (PPy-PVS) film .…”

Section: Acetylcholine (Ach)mentioning

confidence: 99%

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“Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters

Madhurantakam

Karnam

Brabazon

et al. 2020

ACS Chem. Neurosci.

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The growing importance of nanomaterials toward the detection of neurotransmitter molecules has been chronicled in this review. Neurotransmitters (NTs) are chemicals that serve as messengers in synaptic transmission and are key players in brain functions. Abnormal levels of NTs are associated with numerous psychotic and neurodegenerative diseases. Therefore, their sensitive and robust detection is of great significance in clinical diagnostics. For more than three decades, electrochemical sensors have made a mark toward clinical detection of NTs. The superiority of these electrochemical sensors lies in their ability to enable sensitive, simple, rapid, and selective determination of analyte molecules while remaining relatively inexpensive. Additionally, these sensors are capable of being integrated in robust, portable, and miniaturized devices to establish point-of-care diagnostic platforms. Nanomaterials have emerged as promising materials with significant implications for electrochemical sensing due to their inherent capability to achieve high surface coverage, superior sensitivity, and rapid response in addition to simple device architecture and miniaturization. Considering the enormous significance of the levels of NTs in biological systems and the advances in sensing ushered in with the integration of nanotechnology in electrochemistry, the analysis of NTs by employing nanomaterials as interface materials in various matrices has emerged as an active area of research. This review explores the advancements made in the field of electrochemical sensors for the sensitive and selective determination of NTs which have been described in the past two decades with a distinctive focus on extremely innovative attribut,es introduced by nanotechnology.

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Section: ■ Acetylcholine (Ach)mentioning

confidence: 99%

Section: Acetylcholine (Ach)mentioning

confidence: 99%

“Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters

Madhurantakam

Karnam

Brabazon

et al. 2020

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Enzymes are extremely selective and effective compounds, but because of their complicated and fragile structural system, common usage of their exceptional catalytic prospects in biomedical and industrial settings is both desirable and difficult . The invention of new and extremely efficient analytical tools for evaluating the structure of every material that interacts with consumers or nature is being driven by global legislation.…”

Section: Mofs-derived Materials For the Enzymatic Electrochemical Bio...mentioning

confidence: 99%

Metal–Organic Frameworks-Derived Material for Electrochemical Biosensors: Recent Applications and Prospects

Tran

Doan

et al. 2023

Ind. Eng. Chem. Res.

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According to recent studies, interest in metal−organic frameworks (MOFs) and their derivatives are still developing in the field of electrochemical biosensing. This is due to their superior electrical conductivity, active surface area, and easier manufacturing procedures, when compared to other conventional materials. This study discusses current advances in the use of MOF nanoparticles as highly conductive substrates in electrochemical biosensing. Electrochemical biosensing is introduced by categorizing biosensors into enzyme and nonenzyme biosensors and for diagnosis of diseases is based on the application of the active detector. This study systematically compiled and analyzed the most recent published works in each category. This Review provides an overview of the many types of analyses, MOFs materials, and sensor performance. In this subject of science, broad concerns, difficulties, and future views are discussed.

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“…Biosensors make use of various biomarkers (antibodies, enzymes, aptamers, ligands) to gather information regarding some biological, chemical, or physical change and then transform the information into a readable signal. Electrochemical sensors have been considered to be a promising tool due to their fast response time and ability for real-time and on-site detection by generation of an electrochemical signal, and a few have been developed for detection of different AD biomarkers such as acetylcholine enzyme ( Chauhan et al, 2019 ; Chauhan et al, 2020 ) and certain proteins ( Esteves-Villanueva et al, 2014 ; Bungon et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Alzheimer’s Disease Biomarker, β-Secretase Enzyme (BACE1), With One-Step Synthesized Reduced Graphene Oxide

Dey

Roberts

Mahari

et al. 2022

Front. Bioeng. Biotechnol.

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β-Secretase1 (BACE1) catalyzes the rate-limiting step in the generation of amyloid-β peptides, that is, the principal component involved in the pathology of Alzheimer’s disease (AD). Recent research studies show correlation between blood and cerebrospinal fluid (CSF) levels of BACE1 with the pathophysiology of AD. In this study, we report one-step synthesized reduced graphene oxide (rGO), activated via carbodiimide chemistry, conjugated with BACE1 antibody (Ab), and immobilized on fluorine-doped tin oxide (FTO) electrodes for rapid detection of BACE1 antigen (Ag) for AD diagnosis. The synthesis and fabrication steps were characterized using different types of spectroscopic, X-ray analytic, microscopic, and voltametric techniques. Various parameters including nanomaterial/Ab concentration, response time, pH, temperature, and rate of scan were standardized for maximum current output using the modified electrode. Final validation was performed via detection of BACE1 Ag ranging from 1 fM to 1 µM, with a detection limit of 0.64 fM in buffer samples and 1 fM in spiked serum samples, as well as negligible cross-reactivity with neurofilament Ag in buffer, spiked serum, and spiked artificial CSF. The proposed immunosensor gave a quick result in 30 s, and good repeatability and storage stability for a month, making it a promising candidate for sensitive, specific, and early diagnosis of AD. Thus, the fabricated electrochemical biosensor for BACE-1 detection improves detection performance compared to existing sensors as well as reduces detection time and cost, signifying its potential in early diagnosis of AD in clinical samples.

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Bienzymatic assembly formed @ Pt nano sensing framework detecting acetylcholine in aqueous phase

Cited by 38 publications

References 28 publications

“Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters

“Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters

Metal–Organic Frameworks-Derived Material for Electrochemical Biosensors: Recent Applications and Prospects

Electrochemical Detection of Alzheimer’s Disease Biomarker, β-Secretase Enzyme (BACE1), With One-Step Synthesized Reduced Graphene Oxide

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