2022
DOI: 10.3389/fbioe.2022.873811
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Electrochemical Detection of Alzheimer’s Disease Biomarker, β-Secretase Enzyme (BACE1), With One-Step Synthesized Reduced Graphene Oxide

Abstract: β-Secretase1 (BACE1) catalyzes the rate-limiting step in the generation of amyloid-β peptides, that is, the principal component involved in the pathology of Alzheimer’s disease (AD). Recent research studies show correlation between blood and cerebrospinal fluid (CSF) levels of BACE1 with the pathophysiology of AD. In this study, we report one-step synthesized reduced graphene oxide (rGO), activated via carbodiimide chemistry, conjugated with BACE1 antibody (Ab), and immobilized on fluorine-doped tin oxide (FTO… Show more

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Cited by 28 publications
(5 citation statements)
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References 46 publications
(32 reference statements)
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“…These biological interactions can change the sensing interface properties (i.e., capacitance or conductance) of the p-type or ntype transistor. Consequently, in organic transistor biosensors, taking advantage of nanostructures, such as ZnO, MoS2 nanopores, carbon nanotubes, nanowires, copolymers, and graphene sheets (or nanoribbons) on the gate or channel makes them highly sensitive devices for biosensing [83,84]. In addition to enhancing the sensing surface by nanomaterials, a concave surface like crumpled graphene showed higher sensitivity than a flat one [84].…”
Section: A Electrochemical Biosensorsmentioning
confidence: 99%
See 2 more Smart Citations
“…These biological interactions can change the sensing interface properties (i.e., capacitance or conductance) of the p-type or ntype transistor. Consequently, in organic transistor biosensors, taking advantage of nanostructures, such as ZnO, MoS2 nanopores, carbon nanotubes, nanowires, copolymers, and graphene sheets (or nanoribbons) on the gate or channel makes them highly sensitive devices for biosensing [83,84]. In addition to enhancing the sensing surface by nanomaterials, a concave surface like crumpled graphene showed higher sensitivity than a flat one [84].…”
Section: A Electrochemical Biosensorsmentioning
confidence: 99%
“…While transistor-based biosensors commonly offer low power consumption, appropriate sensitivity, and flexibility, the fabrication and biofunctionalization of electrochemical transistors tend to be more complicated than electrode ones. This complexity can also include the necessity for high-precision readout circuits to achieve a high signal-to-noise ratio [83].…”
Section: A Electrochemical Biosensorsmentioning
confidence: 99%
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“…12,13 While there have been reports of highly sensitive sensors [14][15][16][17] developed for JEV antigen (Ag) detection, most of them are electrochemical-based and expensive as well as difficult to fabricate, require skilled handling, and most importantly, are not portable point-of-care (PoC) devices. [18][19][20][21][22][23][24] To overcome these limitations of existing sensors, Ag targeting immunochromatographic lateral ow assays (LFAs) are being developed for rapid bedside detection of various infectious diseases. 25,26 LFAs comprise prefabricated strips of a carrier probe containing dry reagents that are activated by addition of a uid sample, 27 and the absence/presence of the target analyte is indicated by the appearance of coloured lines on the membrane.…”
Section: Introductionmentioning
confidence: 99%
“…These are used as the immobilized biorecognition element (Roberts et al 2021b , 2022b ) on the fabricated sensor such as antibody (Holford et al 2012 ), aptamer (Song et al 2008 ), peptide (Liu et al 2015 ), enzyme (Kim et al 2011 ), etc. Different nanomaterial (Mishra et al 2019 ) for example graphene (Shahdeo et al 2020 ; Dey et al 2022 ; Mahari et al 2022 ), graphitic carbon nitride (Chouhan et al 2021 ), gold (Shahdeo et al 2022 ) etc. may be incorporated as signal enhancers in the biosensing system.…”
Section: Introductionmentioning
confidence: 99%