Biciclogermacreno, resveratrol e atividade antifúngica em extratos de folhas de Cissus verticillata (L.) Nicolson & Jarvis (Vitaceae)

Abstract

RESUMO:Cissus verticillata L. (Vitaceae) tem sido empregada popularmente como antidiabética. Nessa espécie foi também detectada atividade fungitóxica. O objetivo do presente trabalho foi realizar a identifi cação dos compostos fungitóxicos em extratos de folhas. O extrato etanólico foi submetido a fracionamento com solventes de diferentes polaridades (hexano, clorofórmio, acetato de etila e butanol) e seu efeito antifúngico foi analisado frente a Cladosporium sphaerospermum. Os extratos em clorofórmio e acetato de etila mostraram atividade, e foram fracionados por cromatografi a em coluna e cromatografi a preparativa em placas de sílica, respectivamente. Três terpenóides foram isolados do extrato em clorofórmio; um deles foi identifi cado, por análise em CG-EM, como um sesquiterpeno, o biciclogermacreno. O composto ativo do extrato em acetato de etila foi identifi cado, por análises em CLAE, como o estilbeno resveratrol.Unitermos: Cissus verticillata, Vitaceae, atividade fungitóxica, sesquiterpeno, estilbeno.ABSTRACTS: "Bicyclogermacrene, resveratrol and fungitoxic activity on leaves extracts of Cissus verticillata L. Nicolson & Jarvis (Vitaceae)". Cissus verticillata L. (Vitaceae) is popularly employed as hypoglicemic and it shows fungitoxic activity. This work aims at identifi cation of fungitoxic compounds on extracts of leaves. The ethanol extract was partitioned using solvents of different polarities (hexane, chloroform, ethyl acetate and butanol) and its fungitoxic activity was analysed upon Cladosporium sphaerospermum. The chloroform and ethyl acetate extracts showed activity and they were fractionated by column chromatography and preparative TLC, respectively. Three terpenoids were isolated from the chloroform extract, one of them identifi ed by GLC-MS analysis as sesquiterpene bicyclogermacrene. The active compound of the ethyl acetate extract was identifi ed by HPLC analyses as stilbene resveratrol.

Chemical composition and antimicrobial activity of essential oils of Ocimum canum Sims. and Ocimum selloi Benth.

“…These variations are related to environmental factors such as temperature, soil type, moisture, climate, height and factors intrinsic to the plant, as its pathological condition and age , Martins et al 2006, Silva et al 2007b. The content of metabolites may also vary depending on the extraction method used (Charles andSimon 1990, Silva et al 2004).…”

Profile of Essential Oils From the Leaves of Annona Grafted

“…Therefore, α-pinene, β-pinene (Medeiros Leite et al, 2007;Nissen, Zatta, Stefanini, Grandi, & Sgorbati, 2010), limonene (Espina, Gelaw, de Lamo-Castellví, Pagán, & García-Gonzalo, 2013), (E)-caryophyllene (Costa et al, 2008), and spathulenol (Limberger, Sobral, Henriques, Menut, & Bessière, 2004) showed bactericidal activity. β-elemene and spathulenol showed cytotoxic activity (Wang et al, 2005;Yao et al, 2008), β-selinene showed insecticidal and antibacterial activity (Chu et al, 2011;Souza et al, 2017), and bicyclogermacrene showed fungicidal activity (Silva et al, 2007).…”

Trichomes and chemical composition of the volatile oil of Trichogonia cinerea (Gardner) R. M. King & H. Rob. (Eupatorieae, Asteraceae)

“…It is worth to emphasize that some of the constituents identified in this study present therapeutic potential. Sesquiterpene bicyclogermacrene, for instance, exhibited antifungal activity when isolated (Silva et al 2007), whereas (E)-caryophillene showed antibacterial activity (Garg andSiddiqui 1992, Formisano et al 2006). …”