Bi-directionally amplified ratiometric electrochemical aptasensor for the ultrasensitive detection of alpha-fetoprotein

Yang, Xing-Dong; Zhao, Cong-Lin; Zhang, Cuiling; Wen, Ke; Zhu, Ye

doi:10.1016/j.snb.2020.128666

Cited by 35 publications

(19 citation statements)

References 42 publications

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“…Alpha fetoprotein is about 70 kDa in MW, and is produced in the yolk sack, liver, and gastrointestinal tract [272,277]. During cancerous conditions, the alpha fetoprotein concentration is above 500 ng/mL in the human body, while in a healthy body it is only around 20 ng/mL [273,274]. This large abnormality in concentration makes alpha fetoprotein a significant marker for HCC detection.…”

Section: Mnps In Alpha Fetoprotein Sensingmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Mnps In Alpha Fetoprotein Sensingmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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“…An electronic DNA-hairpin beacon, one of the simplest nanoswitching architectures [ 239 ] was designed for 8.76 pg mL −1 detection of AFP; a thiolated and MB-labelled aptamer being immobilised on a gold electrode [ 240 ]. A more complicated design of the bi-directionally amplified ratiometric aptasensor exploited exonuclease-assisted target recycling and a ferrocene-labelled DNA capture probe (Fc-DNA) [ 241 ]. Fc-DNA was hybridised to the thiolated aptamer (DNA1) immobilised on gold, and AFP–aptamer binding resulted in dissociation of Fc-DNA from the hybrid.…”

Section: Electrochemical Assays For Cancer Biomarkersmentioning

confidence: 99%

“…The process of RecJf exonuclease cleavage allowed further recycling of AFP and more Fc-taged DNA released, accompanied by more DNA1 exposed on the electrode surface for hybridisation with a MB-labelled DNA probe. This aptasensor’s LOD reached 0.27 fg mL −1 , and AFP was detected in human serum, with no interference from thrombin, carcinoembryonic antigen, IgG, and PSA [ 241 ]. Another complex redox labelling strategy was proposed for a sandwich aptasensor design, with AFP captured on the thiolated-aptamer-modified gold electrode and covalently labelled by the Michael addition reaction with the maleimide-functionalised DNA probe [ 242 ].…”

Section: Electrochemical Assays For Cancer Biomarkersmentioning

confidence: 99%

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Malecka

Mikuła

Ferapontova

2021

Sensors

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Improved outcomes for many types of cancer achieved during recent years is due, among other factors, to the earlier detection of tumours and the greater availability of screening tests. With this, non-invasive, fast and accurate diagnostic devices for cancer diagnosis strongly improve the quality of healthcare by delivering screening results in the most cost-effective and safe way. Biosensors for cancer diagnostics exploiting aptamers offer several important advantages over traditional antibodies-based assays, such as the in-vitro aptamer production, their inexpensive and easy chemical synthesis and modification, and excellent thermal stability. On the other hand, electrochemical biosensing approaches allow sensitive, accurate and inexpensive way of sensing, due to the rapid detection with lower costs, smaller equipment size and lower power requirements. This review presents an up-to-date assessment of the recent design strategies and analytical performance of the electrochemical aptamer-based biosensors for cancer diagnosis and their future perspectives in cancer diagnostics.

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“…In recent years, there have been many methods for detecting AFP—for example, electrochemiluminescence [ 2 , 3 ], fluorescence immunoassay [ 4 , 5 ], chemiluminescence immunoassay [ 6 ] and enzyme-linked immunoassay [ 7 ]. Electrochemical immunosensors have been widely used in the detection of AFP in recent years due to their high sensitivity, good selectivity and simple instrumentation, which have advantages in the detection of AFP [ 8 , 9 ]. Compared with labeled immunosensors, unlabeled electrochemical immunosensors have attracted more and more attention because of their advantages of simple and convenient preparation [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

Chen

Lin

et al. 2022

Micromachines

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Black phosphorus nanosheets (BPNSs) were synthesized with liquid exfoliation combined with the ultrasonic method and loaded with Fe3+ by simply mixing. The morphology, structure and electrochemical properties of the synthesized Fe3+/BPNSs were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV), etc. The load of Fe3+ can improve the electrochemical performance of BPNSs and enhance the sensitivity of the detection. Additionally, Fe3+/BPNSs display good biocompatibility. In this study, immunosensors based on Fe3+/BPNSs were constructed to detect alpha-fetoprotein (AFP). The detection is due to the specific binding between the AFP antigen and antibody on the surface of the immunosensors, which can reduce the current response of Fe3+/BPNSs. The immunosensors have a good linear relationship in the range of 0.005 ng·mL−1 to 50 ng·mL−1, and the detection limit is 1.2 pg·mL−1. The results show that surface modification with metal ions is a simple and effective way to improve the electrochemical properties of BPNSs, which will broaden the prospects for the future application of BPNSs in the electrochemical field.

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Bi-directionally amplified ratiometric electrochemical aptasensor for the ultrasensitive detection of alpha-fetoprotein

Cited by 35 publications

References 42 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Electrochemical Detection of Alpha-Fetoprotein Based on Black Phosphorus Nanosheets Modification with Iron Ions

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