Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India

Abstract

Aim:The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh.Materials and Methods:A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting blaTEM, blaSHV, blaOXA, blaCTX-M Group 1, 2, blaAmpC, blaACC, and blaMOX genes.Results:A total of 33 Klebsiella and 29 Enterobacter isolates were recovered. Phenotypic beta-lactamase resistance was detected in 66.6% and 25% of Klebsiella and Enterobacter isolates, respectively, from healthy dogs and 66.6% and 60% of Klebsiella and Enterobacter isolates, respectively, from diarrheic dogs. Overall, incidence of extended-spectrum beta-lactamase (ESBL) phenotype was found to be 21.2% (7/33) in Klebsiella isolates, whereas none of the Enterobacter isolates exhibited ESBL phenotype. Predominant beta-lactamase genes detected in Klebsiella species include blaSHV (84.8%), followed by blaTEM (33.3%), blaCTX-M Group 1 (15.1%), and blaOXA (6.1%) gene. Predominant beta-lactamase genes detected in Enterobacter species include blaSHV (48.2%), followed by blaTEM (24.1%), blaAmpC (13.7%), and blaOXA (10.3%) gene.Conclusion:The present study highlighted alarming beta-lactamase resistance in Klebsiella and Enterobacter species of canine origin in India with due emphasis as indicators of antimicrobial resistance.

Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products

“…blaSHV was the predominant gene detected (96.88%, 31/32) followed by blaTEM, blaCTX‐M1, and blaCTX‐M9 (3.12%, 1/32), whereas single K. oxytoca isolate recovered from pork sample harbored blaCTX‐M2 gene. This corroborates with the findings of Sharif et al () who reported predominance of bla SHV (84.8%) over other ESBL genes. Guo et al also reported two ESBL gene carrying K. pneumoniae strains where one isolate had three beta‐lactamases genes (blaSHV, blaCTX‐M‐1, and blaCTX‐M‐10) and the other had the blaSHV gene.…”

“…Resistance to β‐lactam antibiotics is an increasing problem and Extended‐Spectrum β‐lactamases (ESBLs) are variants of beta‐lactamases that confer resistance to the third‐generation cephalosporins. Beta‐lactamase production is mediated by β‐lactamase ( bla ) genes carried on a plasmid or on the chromosome (Sharif, Sreedevi, Chaitanya, & Sreenivasulu, ).…”

Multi‐drug resistant, biofilm‐producing high‐risk clonal lineage of Klebsiella in companion and household animals

“…Among the Klebsiella isolates, 98 strains (98/117, 83Á7%) were confirmed as K. pneumoniae in PCR (Table 1). The prevalence of Klebsiella varied widely (3Á5-26%) in healthy dogs throughout the world depending on the diet, household environment and breed (Donati et al 2014;Sharif et al 2017). Lower prevalence (5Á6%) of Klebsiella was detected in healthy goats in India (Aher et al 2012).…”

“…2014; Sharif et al . 2017). Lower prevalence (5·6%) of Klebsiella was detected in healthy goats in India (Aher et al .…”

Evidence of Sharing of Klebsiella pneumoniae Strains between Healthy Companion Animals and Cohabiting Humans

“…Since this study relied on standard culture procedures, K. pneumoniae colonization and sharing could be underestimated due to the overgrowth of other Enterobacteriaceae species. Nevertheless, a previous study from India has reported that only 26% of the healthy dogs were colonized by K. pneumoniae (45). Considering that 61.5% of participants were colonized by multiple strains, it can be speculated that the PFGE typing of a higher number of colonies per sample could be advantageous in future studies to detect additional sharing pairs.…”