Best Practice in Biological Sample Collection, Processing, and Storage for LC‐MS in Bioanalysis of Drugs

Bioanalytical chromatographic method validation according to current regulations, with a special focus on the non-well defined parameters limit of quantification, robustness and matrix effect

“…Studying the stability of the analyte in stock solutions and matrix is vital to ensure the reliability of the results provided by the analytical method [156]. After the first Crystal City meeting only stability during the collection process, sample storage period and two freeze-thaw cycles was demanded.…”

Efficacy of liquid-liquid extraction and protein precipitation methods in serum sample preparation for quantification of fexofenadine in human serum

“…, 2011; all used protein precipitation techniques for sample preparation in their attempt to quantify fexofenadine in biological matrices. PP is the most commonly used sample preparation method because of its ability to remove the unwanted plasma proteins from samples prior to analysis with minimal method development requirements and low cost 24 . The only drawback it has is it may increase the back pressure of the HPLC system and may affect the column performance 25 .…”

Proteomics for Low Cell Numbers: How to Optimize the Sample Preparation Workflow for Mass Spectrometry Analysis

“…Furthermore, the cell viability medium can include proteins as DNase to avoid cell clumping caused by released DNA. 16 However, it is well-known in the proteomics field that adding extra proteins to the samples is not ideal, as they will interfere with the downstream analysis by hindering the detection of the sample proteins of interest. 17 For this reason, finding the right balance between further protein identification and cell survival is crucial, since dying/apoptotic cells will show a different proteome.…”

“…Nevertheless, the nature of such inhibitors must be considered since they could hamper downstream analysis, e.g., usage of serine protease inhibitors will impair subsequent trypsinization required for shotgun MS, if proper sample washing is not performed before protein digestion. Furthermore, the cell viability medium can include proteins as DNase to avoid cell clumping caused by released DNA . However, it is well-known in the proteomics field that adding extra proteins to the samples is not ideal, as they will interfere with the downstream analysis by hindering the detection of the sample proteins of interest .…”