Bioanalytical chromatographic method validation according to current regulations, with a special focus on the non-well defined parameters limit of quantification, robustness and matrix effect

González, Oskar; Blanco, María M.; Iriarte, Gorka; Bartolomé, L.; Maguregui, Miren Itxaso; Alonso, Rosa M.

doi:10.1016/j.chroma.2014.03.077

Cited by 198 publications

(120 citation statements)

References 138 publications

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“…The fundamental challenge, however, is to find the best analytical conditions for validating the method (i.e. the method is precise and accurate and produces reliable quantitative data for target metabolites in a specific matrix) prior to its implementation in routine analyses [47][48][49]. Nonetheless, one main aspect common to most analytical validation methods is the attempt to maximize the number of known metabolites that can be quantitatively measured in a single study.…”

Section: Analytical Methods Validation For Quantitative Plant Metabolomentioning

confidence: 99%

“…On the other hand, inter-day precision reflects small variations in the experimental or operational conditions from repetitive analysis of the same sample within different days [ The accuracy reflects the closeness of agreement between the true value of the analyte concentration and the mean result obtained by applying the analytical method (i.e. reliable quantification) [48,54,57]. In plant metabolomics studies, where no reference materials with known metabolite concentrations are available, the accuracy of the analytical method can be assessed by determining the analytical recoveries of authentic standards or isotopically labelled metabolites spiked into the plant matrix ( §3f) [56].…”

Section: (C) Methods Precision and Accuracymentioning

confidence: 99%

“…The LOQ is the lowest analyte amount that can be quantitatively determined with a good degree of method precision and accuracy ( §3c). In chromatographic methods, the LOQ can be defined as the concentration for which the S/N equals 10 (figure 3) [48,56].…”

Section: (A) Instrument Linearitymentioning

confidence: 99%

See 2 more Smart Citations

Mass spectrometry as a quantitative tool in plant metabolomics

Jorge

Mata

António

2016

Phil. Trans. R. Soc. A.

110

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Metabolomics is a research field used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore powerful analytical tools are required for the separation, characterization and quantification of this vast compound diversity present in plant matrices. In this review, challenges in the use of mass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and important criteria for the development and validation of MS-based analytical methods provided. This article is part of the themed issue ‘Quantitative mass spectrometry’.

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Section: Analytical Methods Validation For Quantitative Plant Metabolomentioning

confidence: 99%

Section: (C) Methods Precision and Accuracymentioning

confidence: 99%

See 1 more Smart Citation

Mass spectrometry as a quantitative tool in plant metabolomics

Jorge

Mata

António

2016

Phil. Trans. R. Soc. A.

110

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“…[56,63,64]. An overview of these agencies is reviewed by Wille et al and Gonzalez et al [63,64]. The variation on sample precision, accuracy, recovery and MEs should always be below 15% or 20% when working against the limit of quantification (LOQ) [63].…”

Section: (B) Methods Validationmentioning

confidence: 99%

“…Finally, validation experiments can be performed by a different operator to assess robustness [62]. [56,63,64]. An overview of these agencies is reviewed by Wille et al and Gonzalez et al [63,64].…”

Section: (B) Methods Validationmentioning

confidence: 99%

Quantitative mass spectrometry methods for pharmaceutical analysis

Loos¹,

Schepdael²,

Cabooter³

2016

Phil. Trans. R. Soc. A.

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Quantitative pharmaceutical analysis is nowadays frequently executed using mass spectrometry. Electrospray ionization coupled to a (hybrid) triple quadrupole mass spectrometer is generally used in combination with solid-phase extraction and liquid chromatography. Furthermore, isotopically labelled standards are often used to correct for ion suppression. The challenges in producing sensitive but reliable quantitative data depend on the instrumentation, sample preparation and hyphenated techniques. In this contribution, different approaches to enhance the ionization efficiencies using modified source geometries and improved ion guidance are provided. Furthermore, possibilities to minimize, assess and correct for matrix interferences caused by co-eluting substances are described. With the focus on pharmaceuticals in the environment and bioanalysis, different separation techniques, trends in liquid chromatography and sample preparation methods to minimize matrix effects and increase sensitivity are discussed. Although highly sensitive methods are generally aimed for to provide automated multi-residue analysis, (less sensitive) miniaturized set-ups have a great potential due to their ability for in-field usage. This article is part of the themed issue ‘Quantitative mass spectrometry’.

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New Trends in the Analysis of Endocrine Disrupting Compounds in Environmental Samples

Salgueiro‐González

Muniategui-Lorenzo

López-Mahı́a

et al. 2017

Encyclopedia of Analytical Chemistry

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The main aim of this article is to provide an overview of the evolution and trends in the determination of endocrine disrupting compounds (EDCs) in environmental matrices, such as water, sediment, and biota samples. These chemical substances are capable of altering the hormonal system of humans and wildlife at low concentrations, and therefore, continuous investigations based on their occurrence, distribution, and impact are needed to preserve the environment and protect public safety. In order to highlight the gradual change related to the strategies used for EDC determination observed in the past years, this article is divided into four sections: firstly, a brief introduction about the remarkable and emerging EDCs and also the corresponding environmental legislation is included. Then, the state of the determination of EDCs in the environment and the analytical methodologies employed for the analysis of environmental matrices are discussed, with special mention to the new trends in analytical techniques. Finally, future trends required to obtain more data about this topic and control EDCs pollution in the environment are also commented.

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Bioanalytical chromatographic method validation according to current regulations, with a special focus on the non-well defined parameters limit of quantification, robustness and matrix effect

Cited by 198 publications

References 138 publications

Mass spectrometry as a quantitative tool in plant metabolomics

Mass spectrometry as a quantitative tool in plant metabolomics

Quantitative mass spectrometry methods for pharmaceutical analysis

New Trends in the Analysis of Endocrine Disrupting Compounds in Environmental Samples

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