Efficacy of liquid-liquid extraction and protein precipitation methods in serum sample preparation for quantification of fexofenadine in human serum

Moni, Fatema; Sharmin, Suriya; Rony, Satyajit Roy; Afroz, Farhana; Masum, Shah Md.; Sohrab, Hossain

doi:10.23893/1307-2080.aps.6018

Cited by 1 publication

(1 citation statement)

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“…Protein precipitation is the most commonly used sample preparation method because of its ability to remove unwanted plasma proteins from samples before analysis with minimal method development requirements and low cost [32]. The protein precipitation method used in this method requires a low plasma volume of about 200 µl with recovery>95 %, which was higher than the liquid-liquid extraction method reported previously [13,14].…”

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

Development of Quantitative Method of Tulobuterol Hydrochloride in Rat Plasma: Validation and Application to Preclinical Pharmacokinetics

NAZ,

SUBRAHMANYAM,

RACHAMALLA

2023

Int J App Pharm

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Objective: A robust, simple, accurate, rapid, and selective bioanalytical high-performance liquid chromatography (HPLC) method was established and validated to determine the tulobuterol hydrochloride in rat plasma. Methods: The protein precipitation method deproteinated analyte from rat plasma using acetone. The analysis of tulobuterol hydrochloride from rat plasma was accomplished using a mobile phase comprising of methanol: potassium dihydrogen orthophosphate buffer (0.05M; pH 4.0) in 90:10 (v/v) ratio run at 1.0 ml/min flow rate. Separation was carried on BDS hypersil C18 column (4.6 mm × 250 mm; 5 µ) at ambient temperature employing a 996 photodiode array (PDA) detector at 228 nm. Results: The linearity model was exhibited from 100-500 ng/ml with a good correlation of 0.999. Tulobuterol hydrochloride was efficiently separated at a retention time of 7.281 min. The percent recovery rate was between 100.21-100.46 %. The accuracy, precision, robustness, and ruggedness study showed relative standard deviation (%RSD) was within 2% (acceptable limit), and that revealed the method was efficient, precise, reliable, and reproducible. Conclusion: A simple, accurate, suitable method to quantitate tulobuterol hydrochloride in rat plasma was established using HPLC employed with a PDA detector that overcomes the increased cost for analysis. The developed method was successfully validated in rat plasma.

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Section: Pharmacokinetic Studiesmentioning

confidence: 99%