�ber Synergisten von Mitosegiften

Lettr, Hans; Lettr�, Renate; Pflanz, Charlotte

doi:10.1007/bf00624042

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Few labels only are found occasionally in the fibrillar nucleolonema adjacent to fibrillar centers (Figs. 14,15). These are observed usually in cells that show either exclusive or predominant labeling of the dispersed chromatin.…”

Section: Radioautographie Studiesmentioning

confidence: 99%

A Cytochemical and Radioautographic Study of Human Tissue Culture Cell Nucleoli

Recher

Whitescarver

Briggs

1970

The Journal of Cell Biology

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Fine structural aspects of human tissue culture cell nucleoli were studied by cytochemical and radioautographic methods. Ribonuclease and pepsin digestions were carried out on glutaraldehyde-fixed cells that, in some instances, were labeled with thymidine-~H prior to digestion. Double digestion by ribonuclease and pepsin revealed a fine fibrillar reticulum that appears to be the supportive structure of nucleolonemal threads. The nature of the reticulum remains to be determined. The question of whether it may represent a dispersed form of chromatin was raised. Structural findings suggested such an hypothesis but the results of radioautographic studies do not support it. The rcticulum showed a striking absence of radioactive labeling following a 3 hr incorporation of thymidine-3H. Only few silver grains were observed occasionally in the fibrillar nucleolonema that may or may not be significant. The radioautographic results are believed to be inconclusive for the various masons discussed. The possibility that the reticulum is composed of proteins has to be considered. It appears that basic proteins can resist pepsin digestion in aldehyde-fixed cells. Individual chromatin fibrils were found to be associated with the nucleolar reticulum. It is possible that these alone represent the dispersed genetically active chromatin of nucleoli.

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Section: Radioautographie Studiesmentioning

confidence: 99%