Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.

Latza, Ute; Niedobitek, Gerald; Schwarting, Roland; Nekarda, H.; Stein, Harald

doi:10.1136/jcp.43.3.213

Cited by 361 publications

(220 citation statements)

References 32 publications

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“…It has been used in panels to differentiate adenocarcinomas from mesotheliomas (19). In our study, all but one adenocarcinoma and all CC were immunoreactive with Ber-EP4.…”

Section: Discussionsupporting

confidence: 53%

“…The most commonly used markers include antibodies directed against AFP (1, 7), CEA (1, 2), and various cytokeratin subtypes (3)(4)(5)(6)8). Other markers that have been reported as useful include Factor XIII-A (2, 16), hepatitis B surface antigen (17), C-reactive protein (2), ␣-1 antitrypsin (16), HepPar 1 (18), Ber-EP4 (1,19), and molecular probes designed to identify albumin mRNA (20,21).…”

Section: Discussionmentioning

confidence: 99%

“…Albumin mRNA is detected by in situ hybridization. These markers seem to be fairly specific for hepatocytes (18,19), but HepPar 1 is not commercially available, and the detection of albumin mRNA requires in situ hybridization ability, which is expensive, time consuming, and not available in all laboratories. As a result, these markers were not evaluated in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemical panels have been used to separate these tumors with variable success. ␣-Fetoprotein (AFP); polyclonal carcinoembryonic antigen (pCEA); and cytokeratins (CK) 7,8,18,19, and 20 are among some of the more commonly used markers in these panels (1)(2)(3)(4)(5)(6)(7)(8).…”

mentioning

confidence: 99%

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Immunohistochemical Analysis of Hepatocellular and Adenocarcinoma in the Liver: MOC31 Compares Favorably with Other Putative Markers

Young

Proca

et al. 2000

Modern Pathology

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Distinguishing hepatocellular carcinoma (HCC) from metastatic adenocarcinoma (MA) and cholangiocarcinoma (CC) can, at times, be difficult and sometimes requires immunohistochemical analysis. Recently, MOC31, an antibody directed against a cell surface glycoprotein, has been shown to be useful in separating HCC from both MA and CC; however, no study has compared MOC31 and other frequently used immunostains. We compare MOC31 with other commonly used immunostains for HCC, MA, and CC. Formalin-fixed, paraffin-embedded tissue sections from 57 previously characterized hepatic neoplasms (13 HCC, 14 CC, 3 combined HCC-CC, and 27 MA) were immunostained with antibodies directed against MOC31, cytokeratin (CK) 7, CK20, ␣-fetoprotein (AFP), polyclonal carcinoembryonic antigen, Ber-EP4, and Factor XIII-A. Two pathologists reviewed slides, and positivity was defined as more than 1% of cells staining with the appropriate pattern. Positive MOC31 immunostaining was seen in 0 of 13 HCC, 13 of 14 CC, 3 of 3 HCC-CC, and 27 of 27 MA; the staining was strong and diffuse. CK20 reactivity was observed in 0 of 13 HCC, 2 of 14 CC, 0 of 3 HCC-CC, and 12 of 27 MA; CK7 immunostained 4 of 13 HCC, 13 of 14 CC, 3 of 3 HCC-CC, and 15 of 27 MA; AFP was detected in 4 of 13 HCC and 2 of 3 HCC-CC, whereas all CC and MA were negative; polyclonal carcinoembryonic antigen showed immunoreactivity in 12 of 13 HCC and 3 of 3 HCC-CC in a canalicular pattern, whereas diffuse positivity was identified in 13 of 14 CC and 26 of 27 MA; Ber-EP4 immunostained 1 of 13 HCC, 14 of 14 CC, 2 of 3 HCC-CC, and 26 of 27 MA; and Factor XIII-A was negative in all HCC, CC, and MA. MOC31 expression distinguished HCC from adenocarcinoma in 56 of 57 cases. AFP was specific for HCC but was not sensitive. CK7 and CK20 have limited utility in distinguishing HCC from CC or MA, and Factor XIII-A is not useful. Ber-EP4 staining was similar to MOC31, but one HCC did stain with Ber-EP4. Polyclonal CEA yields similar numerical results as MOC31, but the focal nature of the staining and occasional difficulty in evaluating the pattern can make interpretation problematic. We conclude that MOC31 should be a component of the immunohistochemical panel to distinguish HCC from CC and MA.

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“…It has been used in panels to differentiate adenocarcinomas from mesotheliomas (19). In our study, all but one adenocarcinoma and all CC were immunoreactive with Ber-EP4.…”

Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunohistochemical Analysis of Hepatocellular and Adenocarcinoma in the Liver: MOC31 Compares Favorably with Other Putative Markers

Young

Proca

et al. 2000

Modern Pathology

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“…32 A more recent study by Krahl et al 26 indicates absence of BerEP4, a monoclonal antibody recognizing two glycopolypeptides (34 and 39 kDa) found in most human epithelial cells, in all c(13/13) cases of microcystic adnexal carcinoma, and presence in all (28/28) cases of infiltrative basal cell carcinoma, thus attesting to its utility in separating microcystic adnexal carcinoma from infiltrative basal cell carcinoma. 34 The utility of this antibody in distinguishing basal cell carcinoma and squamous cell carcinoma based upon its consistent expression in the former and absence in the latter is well documented. 35,36 While we also found BerEP4 in all cases of infiltrative basal cell carcinoma in our study, we noted strong BerEP4 expression in 38% our microcystic adnexal carcinoma cases.…”

Section: Discussionmentioning

confidence: 99%

Microcystic adnexal carcinoma: an immunohistochemical reappraisal

Hoang

Dresser

Kapur³

et al. 2008

Modern Pathology

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Even though immunohistochemical comparisons of microcystic adnexal carcinoma vs infiltrative basal cell carcinoma and desmoplastic trichoepithelioma exist, they are mostly restricted to the use of a single stain. In addition, a comparison with squamous cell carcinoma has not been reported previously. In this study, we compare the expression of cytokeratin (CK) 15, CK7, CK20, CK903, carcinoembryonic antigen (CEA), CD10, CD15 and BerEP4 in 13 microcystic adnexal carcinoma, eight desmoplastic trichoepithelioma, 10 infiltrative basal cell carcinoma, and eight squamous cell carcinoma of which five exhibited ductal differentiation. We found that the majority of microcystic adnexal carcinoma (92%) and desmoplastic trichoepithelioma (100%) cases expressed CK15 while the infiltrative basal cell carcinoma and squamous cell carcinoma cases were all negative. Forty percent of infiltrative basal cell carcinoma expressed CK7; while only two microcystic adnexal carcinoma cases (15%) and one squamous cell carcinoma with ductal differentiation (12%) expressed CK7 in the remaining three tumor categories. None of the desmoplastic trichoepithelioma expressed CK7. All tumors were strongly positive for CK903. While the neoplastic cells were negative, luminal staining of ductal structures was noted for CK7, CD15 and CEA in some of the microcystic adnexal carcinoma, desmoplastic trichoepithelioma and squamous cell carcinoma with ductal differentiation cases. Sixty percent of infiltrative basal cell carcinoma, 31% of microcystic adnexal carcinoma, and 25% of squamous cell carcinoma express CD10. BerEP4 expression was noted in 38% of microcystic adnexal carcinoma, 57% of desmoplastic trichoepithelioma, 100% of infiltrative basal cell carcinoma, and 38% of squamous cell carcinoma. In conclusion, we found CK15 to be a useful marker in distinguishing microcystic adnexal carcinoma from infiltrative basal cell carcinoma and squamous cell carcinoma with ductal differentiation. Our experience indicates that microcystic adnexal carcinoma and desmoplastic trichoepithelioma have a similar immunohistochemical profile that is, CK15 þ and BerEP4 þ /À; thus, additional studies are needed to separate these two entities.

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Nodular basal cell carcinoma in vivo vs in vitro. Establishment of pure cell cultures, cytomorphologic characteristics, ultrastructure, immunophenotype, biosynthetic activities, and generation of antisera

Grando

Schofield

Skubitz³

et al. 1996

Archives of Dermatology

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Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.

Cited by 361 publications

References 32 publications

Immunohistochemical Analysis of Hepatocellular and Adenocarcinoma in the Liver: MOC31 Compares Favorably with Other Putative Markers

Immunohistochemical Analysis of Hepatocellular and Adenocarcinoma in the Liver: MOC31 Compares Favorably with Other Putative Markers

Microcystic adnexal carcinoma: an immunohistochemical reappraisal

Nodular basal cell carcinoma in vivo vs in vitro. Establishment of pure cell cultures, cytomorphologic characteristics, ultrastructure, immunophenotype, biosynthetic activities, and generation of antisera

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