1996
DOI: 10.1001/archderm.132.10.1185
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Nodular basal cell carcinoma in vivo vs in vitro. Establishment of pure cell cultures, cytomorphologic characteristics, ultrastructure, immunophenotype, biosynthetic activities, and generation of antisera

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Cited by 19 publications
(23 citation statements)
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“…This discrepancy between in vitro and in vivo growth capacity in our system is not surprising, because even tumor-derived cells of fast growing tumors such as basal cell carcinomas were difficult to propagate in culture. 56,57 There are a few examples in the literature describing an in vitro progression to a more malignant phenotype. 19,24 However, this in vitro progression either occurred in simian virus-40 immortalized cell lines, 19 that are characterized by an increased genetic instability, or required the action of an additional mutagen such as radiation.…”
Section: Discussionmentioning
confidence: 99%
“…This discrepancy between in vitro and in vivo growth capacity in our system is not surprising, because even tumor-derived cells of fast growing tumors such as basal cell carcinomas were difficult to propagate in culture. 56,57 There are a few examples in the literature describing an in vitro progression to a more malignant phenotype. 19,24 However, this in vitro progression either occurred in simian virus-40 immortalized cell lines, 19 that are characterized by an increased genetic instability, or required the action of an additional mutagen such as radiation.…”
Section: Discussionmentioning
confidence: 99%
“…6 Other studies have revealed a high overlap of chromosomal aberrations in both epithelial and spindle-cell components, suggesting that divergence probably occurred late in tumorigenesis. 7 In 1996, Grando et al 8 demonstrated that BCC could differentiate into a mesenchymal phenotype in vitro. Similarly, in 2002, Carlson et al 9 showed BCC transforming into sarcoma in severe combined immunodeficient beige mice transplanted with human BCC cells.…”
Section: Reportmentioning
confidence: 99%
“…Four primary basal cell carcinoma cell cultures were established according to the previously described methodology (11). These basal cell carcinoma cell lines exhibited a low culture-doubling rate, ranging from 14 to 30 days.…”
Section: Cell Linesmentioning
confidence: 99%
“…They were negative for expression of vimentin, neuron-specific enolase, and S-100. To remove contaminating keratinocytes, the primary basal cell carcinoma cultures were exposed to "calcium shift" (11). The increase in calcium concentration in growth medium led to their cornification in the next 96 h, whereas basal cell carcinoma cells flattened, elongated, and stratified but neither cornified nor died (11).…”
Section: Cell Linesmentioning
confidence: 99%