Beiträge zur Kenntnis der Gitterfasern mit besonderer Berücksichtigung der Niere

Krauspe, C

doi:10.1007/bf01944114

Cited by 36 publications

(1 citation statement)

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“…Some considered it as a hyaline or homogeneous membrane (Mall, 1896;Ebner, 1902;Regaud and Policard, 1903-04;Krauspe, 1922;Schaffer, 1927;M6llendorff, 1927), others as a fibrillar structure (Riihle, 1897;Russakoff, 1909) or as a granular band (Bargmann, 1929). Comprehensive studies by McGregor in 1929 did not reveal either a definite fibrillar or homogeneous structure.…”

Section: Formation Of the Basement Membranementioning

confidence: 99%

Studies on developing retinal vessels. X. Formation of the basement membrane and differentiation of intramural pericytes.

Shakib¹,

Oliveira²

1966

British Journal of Ophthalmology

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PREVIOUS studies (Ashton, Ward, andSerpell, 1953, 1954), have demonstrated that the kitten is an ideal animal for studying developing retinal vessels. Since these vessels reach the periphery of the retina about three weeks after birth, all stages of their development can be studied in the same eye.The present study deals with the origin of the intramural pericytes and the formation of the basement membrane of the retinal vessels. Material and MethodsNormal retinae from kittens 1 to 8 days old were used in this investigation.For light microscopical study the specimens were digested with pepsin-trypsin technique (Ashton, 1963) and stained with periodic acid-Schiff and haematoxylin (PASH).For electron microscopical study the animals were anaesthetized by intraperitoneal injections of Nembutal, and their eyes were enucleated and rapidly opened by a coronal section through the ora serrata. The vitreous was gently removed; the posterior half of the eye was then cut into four pieces and immersed in chilled 1 per cent. isotonic veronal buffered osmium tetroxide. In order to allow better penetration of the fixative the retina was separated from the choroid with a thin spatula. The fixation was carried out for two hours at 4°C. temperature. The retinae were dehydrated in graded concentrations of alcohol and embedded in Epon and Araldite. Sections 1 t thick were cut from the whole block, using a Huxley microtome, stained with 1 per cent. alkaline toluidine blue, and studied by light microscopy. Thin sections were cut from selected areas of the block, stained with uranyl acetate in acetic acid, followed by 1 per cent. lead citrate, and viewed by an A.E.I. EM6 electron microscope. ObservationsLight Microscopy.-Normal developing retinal vessels in the kitten show a vascular network comprising a pattern of polygonal spaces, usually pentagonal, which are larger on the arterial side of the capillary circulation and gradually *

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Section: Formation Of the Basement Membranementioning

confidence: 99%