As indicated iii the preceding paper, the mitochoiidria of the liver of the guinea pig and rabbit are wholly insoluble in a 0.85 solution of sodium chloride. In order to obtain mitochondria for examination, it is only necessary to extract by diff erential centrifugation the emulsion of guinea pig liver described in the previous article.The emulsion is prepared by grinding or crushing in a mortar fresh liver which has been it-ashed free of blood by perfusion, or by kneading through bolting silk. This emulsion, as stated in a previous paper, contains fragments of small aggregations of liver cells, individual liver cells in great abundance, large numbers of nuclei released from cytoplasm, a few blood corpuscles and much reticulum arid mitochondria. The salt solution used for this preparation has a p H of about 6, so has a slightly acid reaction. Distinctly alkaline Ringer solntions are quite unsuited for the purpose and salt solutions made with conductivity wafer arc not so successful as solutions made with ordinary distilled water, having the reaction indicated above.Centrifugation of this cmulsion at low speed for a period of 3 niinntes sedimenls the majority of cell masses. If an attempt be made to get out the suspended cells at one operation, a great loss of mitochondria ensues, so it is better to rid the suspension of the groups of intact cells, individual '
When we consider the immense amount of energy that has been expended on the investigation of the gastric glands since the discovery of the chief cells by Heidenhain and Rollet in 1810, it is a matter for some surprise that so little interest should have been aroused in the small zone of glands surrounding the cardiac orifice of the stomach, now generally known as the cardiac glands. This fact is all the more surprising when we remember that, in some mammals, these glands are by no means restricted to the narrow zone mentioned above, but may equal or even exceed in number the ordinary fundus glands composed of chief and parietal cells.
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