Beige mouse model for Mycobacterium avium complex disease

Gangadharam, P. R. J.

doi:10.1128/aac.39.8.1647

Cited by 35 publications

(32 citation statements)

References 65 publications

(77 reference statements)

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“…BALB/c mice immunosuppressed with either cyclophosphamide or prednisolone also establish an intestinal infection following oral M. paratuberculosis inoculation (113). Beige mice are derived from C57/B6 mice and are deficient in lysosomal granules, type 2 pneumocytes and mast cells, and natural killer (NK) cells (122). Phenotypically, these deficiencies make them more susceptible to pyogenic bacterial infections.…”

Section: Animal Modelsmentioning

confidence: 99%

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Mycobacterium aviumsubsp.paratuberculosisin Veterinary Medicine

2001

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Mycobacterium avium subsp. paratuberculosis (basonym M. paratuberculosis) is the etiologic agent of a severe gastroenteritis in ruminants known as Johne's disease. Economic losses to the cattle industry in the United States are staggering, reaching $1.5 billion annually. A potential pathogenic role in humans in the etiology of Crohn's disease is under investigation. In this article, we review the epidemiology, pathogenesis, diagnostics, and disease control measures of this important veterinary pathogen. We emphasize molecular genetic aspects including the description of markers used for strain identification, diagnostics, and phylogenetic analysis. Recent important advances in the development of animal models and genetic systems to study M. paratuberculosis virulence determinants are also discussed. We conclude with proposals for the applications of these models and recombinant technology to the development of diagnostic, control, and therapeutic measures

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Section: Animal Modelsmentioning

confidence: 99%

“…Phenotypically, these deficiencies make them more susceptible to pyogenic bacterial infections. Beige mice have been utilized extensively to study M. avium infections and experimental chemotherapy in AIDS patients (21,22,122). When inoculated intravenously with ca.…”

Section: Animal Modelsmentioning

confidence: 99%

Mycobacterium aviumsubsp.paratuberculosisin Veterinary Medicine

2001

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“…The studies of NTM pathogenesis and host immunity to NTM have been hampered by the lack of a robust animal model that recapitulates the hallmark of human nondisseminated MAC pulmonary disease. Intratracheal or nasal inoculation of golden Syrian hamsters, immunocompetent C57BL/6 and BALB/c mice, and immunocompromised beige mice results in rapid dissemination from the lungs to other organs (9)(10)(11)(12). Consequently, our understanding of the host immune response during NTM infection has largely been derived from the study of disseminated mycobacterial disease (13).…”

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confidence: 99%

A Rhesus Macaque Model of Pulmonary Nontuberculous Mycobacterial Disease

Winthrop

Rivera

Engelmann

et al. 2016

Am J Respir Cell Mol Biol

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In this study, we sought to develop a nonhuman primate model of pulmonary Mycobacterium avium complex (MAC) disease. Blood and bronchoalveolar lavage fluid were collected from three female rhesus macaques infected intrabronchially with escalating doses of M. avium subsp. hominissuis. Immunity was determined by measuring cytokine levels, lymphocyte proliferation, and antigen-specific responses. Disease progression was monitored clinically and microbiologically with serial thoracic radiographs, computed tomography scans, and quantitative mycobacterial cultures. The animal subjected to the highest inoculum showed evidence of chronic pulmonary MAC disease. Therefore, rhesus macaques could provide a robust model in which to investigate host-pathogen interactions during MAC infection.

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“…with 0n2 ml of a bacterial suspension containing 1i10( ST c.f.u. (Gangadharam, 1995 ;Fattorini et al, 1998). One day after infection, five mice were killed, and the organs were aseptically removed, homogenized in 1.5 ml Middlebrook 7H9 broth and sonicated for 10 s. To enumerate c.f.u., appropriate dilutions of the homogenates were plated onto Middlebrook 7H10 agar and, after 2 weeks incubation at 37 mC under a humidified 5 % CO # atmosphere, colonies were counted.…”

Section: Micro-organism and Isolation Of Colonial Variantsmentioning

confidence: 99%

Virulence and drug susceptibility of Mycobacterium celatum

Fattorini

Baldassarri

et al. 2000

Microbiology

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The virulence and drug susceptibility of a clinical isolate of Mycobacterium celatum which showed smooth transparent (ST) and smooth opaque (SO) colonies were studied. While ST cells multiplied intracellularly and maintained their coccobacillary form in a human macrophage model of infection, SO cells formed long filaments and completely destroyed the phagocytes. In BALB/c mice, the ST variant, but not the SO variant, grew efficiently in the spleen, liver and lung. The ST variant was usually more resistant in vitro than the SO variant to drugs, with MIC values for clarithromycin (CLA), azithromycin (AZI), ciprofloxacin, sparfloxacin, amikacin, clofazimine, ethambutol and isoniazid being higher than those of the SO variant. In beige mice infected with the more highly virulent variant ST, CLA and AZI were the most active drugs in terms of viable count reduction in organs and mutant selection. Together, these observations indicate that the ST variant of M. celatum is a virulent form that can be efficiently inhibited in vivo by CLA and AZI.

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Beige mouse model for Mycobacterium avium complex disease

Cited by 35 publications

References 65 publications

Mycobacterium aviumsubsp.paratuberculosisin Veterinary Medicine

Mycobacterium aviumsubsp.paratuberculosisin Veterinary Medicine

A Rhesus Macaque Model of Pulmonary Nontuberculous Mycobacterial Disease

Virulence and drug susceptibility of Mycobacterium celatum

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