Virulence and drug susceptibility of Mycobacterium celatum

Fattorini, Lanfranco; Baldassarri, Lucilla; Li, Yongjun; Ammendolia, Maria Grazia; Fan, Yuming; Recchia, Simona; Iona, Elisabetta; Orefici, Graziella

doi:10.1099/00221287-146-11-2733

Cited by 16 publications

(7 citation statements)

References 28 publications

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“…It grows in 20 to 35 days, between 33 and 42°C, forming small, smooth, colonies described as unpigmented, but the majority of the strains are pale yellow and scotochromogenic. Polymorphic colonies have also been reported (39). The most important distinctive biochemical test seems to be the positive arylsulfatase activity (Table 1).…”

Section: Celatummentioning

confidence: 98%

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

2003

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The advancement of genetic techniques has greatly boosted taxonomic studies in recent years. Within the genus Mycobacterium, 42 new species have been detected since 1990, most of which were grown from clinical samples. Along with species for which relatively large numbers of strains have been reported, some of the new species of mycobacteria have been detected rarely or even only once. From the phenotypic point of view, among the new taxa, chromogens exceed nonchromogens while the numbers of slowly and rapidly growing species are equivalent. Whereas conventional identification tests were usually inconclusive, an important role was played by lipid analyses and in particular by high-performance liquid chromatography. Genotypic investigations based on sequencing of 16S rRNA gene have certainly made the most important contribution. The investigation of genetic relatedness led to the redistribution of the species previously included in the classically known categories of slow and rapid growers into new groupings. Within slow growers, the intermediate branch related to Mycobacterium simiae and the cluster of organisms related to Mycobacterium terrae have been differentiated; among rapid growers, the group of thermotolerant mycobacteria has emerged. The majority of species are resistant to isoniazid and, to a lesser extent, to rifampin. Many of the new species of mycobacteria are potentially pathogenic, and there are numerous reports of their involvement in diseases. Apart from disseminated and localized diseases in immunocompromised patients, the most frequent infections in immunocompetent people involve the lungs, skin, and, in children, cervical lymph nodes. The awareness of such new mycobacteria, far from being a merely speculative exercise, is therefore important for clinicians and microbiologists

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Section: Celatummentioning

confidence: 98%

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

2003

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“…Mycobacterium celatum was first described in 1993 as a new mycobacterial species with a mycolic acid pattern closely related to that of Mycobacterium xenopi (Butler et al, 1993). The infections caused by this organism were reported to occur mostly in persons with suppressed cell-mediated immunity (Tortoli et al, 1995;Piersimoni et al, 1997;Gholizadeh et al, 1998;Fattorini et al, 2000), but some infections also occurred in the immunocompetent host (Bux-Gewehr et al, 1998;Fattorini et al, 2000;Piersimoni et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The organization of two rRNA (rrn) operons of the slow-growing pathogenMycobacterium celatumprovides key insights into mycobacterial evolution

Stadthagen-Gomez¹,

Helguera-Repetto²,

Cerna-Cortés³

et al. 2008

FEMS Microbiology Letters

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The slow-growing Mycobacterium celatum is known to have two different 16S rRNA gene sequences. This study confirms the presence of two rrn operons and describes their organization. One operon (rrnA) was found to be located downstream from murA and the other (rrnB) was found downstream from tyrS. The promoter regions were sequenced, and also the intergenic transcribed spacer (ITS1 and ITS2) regions separating the 16S rRNA, 23S rRNA and 5S rRNA gene coding regions. Analysis of the RNA fraction revealed that rrnA is regulated by two (P1 and PCL1) promoters and rrnB is regulated by one (P1). These data show that the two rrn operons of M. celatum are organized in the same way as the two rrn operons of classical fast-growing mycobacteria. This information was incorporated into a phylogenetic analysis of the genus based on both 16S rRNA gene sequences and (where possible) the number of rrn operons per genome. The results suggest that the ancestral Mycobacterium possessed two (rrnA and rrnB) operons per genome and that subsequently, on two separate occasions, an operon (rrnB) was lost, leading to two clusters of species having a single operon (rrnA); one cluster includes the classical pathogens and the other includes Mycobacterium abscessus and Mycobacterium chelonae.

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“…Its detection in animals is even less frequent and only two cases of M. celatum infection in domestic ferret (Mustela putorius furo) and one in a white-tailed trogon (Trogon viridis) have been reported [40]. Even though mice can be susceptible to M. celatum during experimental infection [41], this is the first report on the detection of this species in free-living rodents. It is worth mentioning that M. celatum can cause false M. tuberculosis-positive results in commercial molecular identification tests [9].…”

Section: Discussionmentioning

confidence: 85%

Detection of Wood Mice (Apodemus sylvaticus) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis

et al. 2020

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Mycobacterial infections caused by the Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacteria (NTM) are of great medical and veterinary relevance. The aim of this research was to study whether small mammals play a role in the epidemiology of mycobacterioses. Four samplings of 100 traps were performed in each of three cattle farms with previous history of tuberculosis or NTM between 2017 and 2018. A total of 108 animals belonging to seven species were trapped, classified, and necropsied, and tissues were submitted to microbiological and molecular methods for mycobacteria identification. The wood mouse (Apodemus sylvaticus) was the most abundant species (87%). No MTC was detected but six different NTM were identified (M. intracellulare, M. avium subsp. paratuberculosis, M. gordonae, M. celatum, M. fortuitum, and a not determined Mycobacterium sp.), showing a prevalence of 6.5%. No significant association was found between mycobacteria prevalence and the analyzed factors. Although a role in the epidemiology of MTC could not be attributed to small mammals, A. sylvaticus carries NTM that could be pathogenic or interfere with the diagnosis of tuberculosis. According to our results, there is a risk of NTM transmission at the wildlife–livestock interface through potential indirect contacts between small mammals and cattle.

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Virulence and drug susceptibility of Mycobacterium celatum

Cited by 16 publications

References 28 publications

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

The organization of two rRNA (rrn) operons of the slow-growing pathogenMycobacterium celatumprovides key insights into mycobacterial evolution

Detection of Wood Mice (Apodemus sylvaticus) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis

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