Behavior of Two and Three Electrode Configuration and Different Mediators in Working Electrode on Development of Disposable Screen-Printing Biosensors for Determination of Free Cholesterol

Abstract

In the present work the development of an amperometric transducer in order to build a free cholesterol biosensor in planar configuration is reported. The one single use disposable biosensors were constructed by screen printing process which is compatible with automated methodologies of production. Based in the incorporation of tetracyanoquinodimethane (TCNQ), Prussian blue (PB) or ferrite (Fe<sub>3</sub>O<sub>4</sub>) as mediator or electrocatalytic agent, three types of electrochemical transducers were evaluated. The cholesterol biosensors require a sample volume of 7.2 μL, exhibits good reproducibility and selectivity and cover a lineal answer of 2-16 mM with detection limits from 0.3 to 1.6 mM. The characteristics of biosensors are satisfactory for the decentralized analysis of the lipid in blood since enclose the range of clinical interest (3.5-6.5 mM).

Nanoarchitectonics with NADPH Catalyst and Quantum Dots Copper Sulfide on Titanium Dioxide Nano-sheets Electrode for Electrochemical Biosensing of Sorbitol Detection

“…The reproducibility of the AR-NADPH/CuS/Ti-TiO 2 were also evaluated by measuring the current response s relative standard deviation (RSD) from the standard deviation of (Epa, Epc, Ipa, Ipc and ΔEp) as shown in Table 1, which were found close to the result reported by CONGO and TAI amperometric transducer 48) . Mónica Hernández-Cruz et al 49) observed that the mean peak potential separation between the anodic and cathodic peak (ΔEp) was similar to that of an ideal amperometric transducer.…”

Desarrollo y Evaluación de un Potenciostato Electrónico Digital de Bajo Costo para la Cuantificación de Sustancias Químicas Mediante Biosensores Amperométricos

“…Cuando el elemento de reconocimiento es de origen biológico (enzimas, anticuerpos, etc. ), el sensor químico es denominado biosensor (Hernández, 2013). Para trabajar con biosensores electroquímicos se requiere de un potenciostato, el cual es un instrumento que permite imponer un potencial fijo a un electrodo (biosensor), para producir la perturbación deseada y registrar simultáneamente la intensidad de corriente, el potencial y el tiempo en el que transcurre.…”