Beads of collagen–nanohydroxyapatite composites prepared by a biomimetic process and the effects of their surface texture on cellular behavior in MG63 osteoblast-like cells

Bone Physiology, Biomaterial and the Effect of Mechanical/Physical Microenvironment on Mesenchymal Stem Cell Osteogenesis

“…This observation is consistent with the fact that bone itself is a structure with a nano-rough surface, which mainly consists of 90% type I collagen synthesized by osteoblasts with linear fibrils 300 nm in length and 0.5 nm in diameter [79], and of hydroxyapatite crystals matrices that contain hexagonal unit structures 2~5 nm thick and 20~80 nm long [80]. Accordingly, current research on nanotexture surfaces aims to reproduce the natural nanoroughness of bone using nanotechnology techniques [73, 78, 81]. In the future, studies can also focus on cell substrate interactions at the atomic and molecular level to allow the study of the ultrastructure levels [72, 82].…”

Enhancing Alendronate Release from a Novel PLGA/Hydroxyapatite Microspheric System for Bone Repairing Applications

“…The lysate solution was incubated with p-nitrophenyl phosphate (pNPP) solution at 37°C for 30 min and then subjected to a spectrophotometer on which the absorbance at 405 nm was measured and recorded to indicate ALP concentration (26).…”

In vitro Biomimetic Construction of Hydroxyapatite–Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture

“…13,21,22 The other method involves a porous collagen material obtained through the cross-linking of 1 purified collagen in solution and a lyophilization technique, where a layer of HAp nanostructure is then coated by co-precipitation or mineralization. [23][24][25] Method 1 can provide accurate quantity of the HAp content in the scaffold, whereas method 2 can achieve controlled growth of HAp on collagen. However, both methods are related to soluble pure collagen type I, which is mainly obtained from rat-tail tendon, bovine tendon, or skin.…”