<i>In vitro</i> Biomimetic Construction of Hydroxyapatite–Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture

Zhao, Hongxin; Wang, Guancong; Hu, Shunpeng; Cui, Jingjie; Ren, Na; Liu, Duo; Liu, Hong; Cao, Chengbo; Wang, Jing; Wang, Zhong Lin

doi:10.1089/ten.tea.2010.0196

Cited by 31 publications

(37 citation statements)

References 54 publications

(51 reference statements)

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“…This HAp-PADM scaffold has both interconnected channels (∼100 µm in diameter) formed by collagen fiber bundles and nanopores (,100 nm in diameter). 6 In this study, the effect of this surface biomimetic apatite nanonetwork structure of PADM on the biological characteristics and osteogenic differentiation potential of PDLSCs was examined. These characteristics were compared with PDLSCs cultured on PADM with a smooth surface.…”

Section: Discussionmentioning

confidence: 99%

“…16 By the assembly of HAp on PADM, the obtained HAp-PADM scaffold has a two-level porous structure with large channels (∼100 µm in diameter) inherited from the purified PADM microstructure and small pores (100-120 nm in diameter) made up of self-assembled HAp on the channel surfaces. 6 The nano-HAp porous structure and calcium element on the channel wall may alter the phenotypic expression of PDLSCs.…”

Section: Discussionmentioning

confidence: 99%

“…The preparation process, structural and morphological characterization, mechanical properties, and in vitro biodegradation rate of PADM and HAp-PADM scaffolds were described in detail previously. 6 PADM is a 3D interconnected network structure, mainly composed of collagen I, with channel diameter over 100 µm and channel wall thickness of about 10-20 µm. The HAp-PADM composite scaffold is a two-level, 3D network structure.…”

Section: Preparation Of Padm and Hap-padm Scaffoldsmentioning

confidence: 99%

“…11,12 Currently, by a biomimetic mineralization method, the authors' group and Guo et al successfully assembled HAp on the channel surface of PADM, and prepared a bioactive scaffold for periodontal tissue engineering (HAp-PADM). 6,13 It was found that HAp-PADM had relatively high mechanical strength and reduced the high rate of enzymatic degradation of natural collagen, which could increase the compressive resistance and stabilize the collagen-based materials. Moreover, the HAp-PADM scaffold was biocompatible for human periodontal ligament (PDL) cells and MC3T3-E1 preosteoblasts, and the cells were able to proliferate and migrate into the scaffold.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the HAp-PADM scaffold was biocompatible for human periodontal ligament (PDL) cells and MC3T3-E1 preosteoblasts, and the cells were able to proliferate and migrate into the scaffold. 6,13 Therefore, this composite system combining the advantages of PADM and HAp seems to be a promising choice for periodontal tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Effects of hydroxyapatite nanostructure on channel surface of porcine acellular dermal matrix scaffold on cell viability and osteogenic differentiation of human periodontal ligament stem cells

Ge¹,

Zhao²,

Wang³

et al. 2013

IJN

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Abstract:A new nanostructured hydroxyapatite-coated porcine acellular dermal matrix (HAp-PADM) was fabricated by a biomimetic mineralization method. Human periodontal ligament stem cells were seeded on HAp-PADM and the effects of this scaffold on cell shape, cytoskeleton organization, cell viability, and osteogenic differentiation were examined. Periodontal ligament stem cells cultured on HAp-PADM exhibited different cell shape when compared with those on pure PADM. Moreover, HAp-PADM promoted cell viability and alkaline phosphatase activity significantly. Based on quantitative real-time polymerase chain reaction, the expression of bonerelated markers runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) upregulated in the HAp-PADM scaffold. The enhancement of osteogenic differentiation of periodontal ligament stem cells on the HAp-PADM scaffold was proposed based on the research results. The results of this study highlight the micro-nano, two-level, three-dimensional HAp-PADM composite as a promising scaffold for periodontal tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Padm and Hap-padm Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of hydroxyapatite nanostructure on channel surface of porcine acellular dermal matrix scaffold on cell viability and osteogenic differentiation of human periodontal ligament stem cells

Ge¹,

Zhao²,

Wang³

et al. 2013

IJN

Self Cite

View full text Add to dashboard Cite

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Materials‐Mediated In Situ Physical Cues for Bone Regeneration

Liu,

Zhang,

et al. 2023

Adv Funct Materials

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Physical cues like morphology, light, electric signal, mechanic signal, magnetic signal, and heat can be used as alternative regulators for expensive but short‐acting growth factors in bone tissue engineering to promote osteogenic differentiation and bone regeneration. As physical stimulation applied directly to the tissue cannot be focused on the bone defect area to regulate the cell behaviors and fate in situ, this limits the efficiency of precise bone regeneration. Biomaterials‐mediated in situ physical cues, as an effective strategy combining the synergistic effect of materials themselves, are put forward and studied widely to promote osteogenic differentiation and bone repair efficiently and precisely. Different types of physical cues provide different choices to better satisfy the requirements for targeted bone defect repair. In this review, the recent research about different biomaterials‐mediated physical cues accelerating osteogenesis in vitro and promoting in situ bone formation in vivo is introduced. Meanwhile, the corresponding possible mechanisms of various physical cues regulating cell responses are also discussed. This review provides useful and enlightening guidance for the utilization of intrinsically physical properties of functional materials to achieve efficient bone regeneration, leading to the design and construction of smart biomaterials for practical applications, and eventually promoting clinical translation.

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Biopolymer/Calcium Phosphate Scaffolds for Bone Tissue Engineering

Baker

Mou

et al. 2013

Adv Healthcare Materials

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With nearly 30 years of progress, tissue engineering has shown promise in developing solutions for tissue repair and regeneration. Scaffolds, together with cells and growth factors, are key components of this development. Recently, an increasing number of studies have reported on the design and fabrication of scaffolding materials. In particular, inspired by the nature of bone, polymer/ceramic composite scaffolds have been studied extensively. The purpose of this paper is to review the recent progress of the naturally derived biopolymers and the methods applied to generate biomimetic biopolymer/calcium phosphate composites as well as their biomedical applications in bone tissue engineering.

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In vitro Biomimetic Construction of Hydroxyapatite–Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture

Cited by 31 publications

References 54 publications

Effects of hydroxyapatite nanostructure on channel surface of porcine acellular dermal matrix scaffold on cell viability and osteogenic differentiation of human periodontal ligament stem cells

Effects of hydroxyapatite nanostructure on channel surface of porcine acellular dermal matrix scaffold on cell viability and osteogenic differentiation of human periodontal ligament stem cells

Materials‐Mediated In Situ Physical Cues for Bone Regeneration

Biopolymer/Calcium Phosphate Scaffolds for Bone Tissue Engineering

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