BcPME37c is involved in pollen intine formation in Brassica campestris

Xiong, Xingpeng; Zhou, Dong; Xu, Liai; Liu, Tingting; Yue, Xiumei; Liu, Weimiao; Cao, Jiashu

doi:10.1016/j.bbrc.2019.07.009

Cited by 18 publications

(9 citation statements)

References 32 publications

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“…Aspartic protease participates in stamen development and cell death in rice ( Niu et al, 2013 ; Ko et al, 2014 ) and PME genes are involved in pollen development and pollen tube growth in B . campestris ( Xiong et al, 2019 ). In the MF category, the dominant groups were binding (49%) and catalytic activity (44%).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the plant-type cell wall term (GO:0009505), seven aspartic proteinase (AP) and nine pectin methyl-esterase (PME) DEGs were enriched in this term (Supplementary Figure 7A). Aspartic protease participates in stamen development and cell death in rice (Niu et al, 2013;Ko et al, 2014) and PME genes are involved in pollen development and pollen tube growth in B. campestris (Xiong et al, 2019). In the MF category, the dominant groups were binding (49%) and catalytic activity (44%).…”

Section: Go and Kegg Analyses Of Degsmentioning

confidence: 99%

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Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Yang

Nong

et al. 2021

Front. Plant Sci.

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Crosses that lead to heterosis have been widely used in the rapeseed (Brassica napus L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility (Rf) systems represent one of the most useful tools for rapeseed production. Several CMS types and their restorer lines have been identified in rapeseed, but there are few studies on the mechanisms underlying fertility restoration. Here, we performed morphological observation, map-based cloning, and transcriptomic analysis of the F2 population developed by crossing the CMS line WNJ01A with its restorer line Hui01. Paraffin-embedded sections showed that the sporogenous cell stage was the critical pollen degeneration period, with major sporogenous cells displaying loose and irregular arrangement in sterile anthers. Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F2 buds, respectively, we identified a 6.25 Mb candidate interval where Rfw is located. Using map-based cloning experiments combined with bacterial artificial chromosome (BAC) clone sequencing, the candidate interval was reduced to 99.75 kb and two pentatricopeptide repeat (PPR) genes were found among 28 predicted genes in this interval. Transcriptome sequencing showed that there were 1679 DEGs (1023 upregulated and 656 downregulated) in fertile compared to sterile F2 buds. The upregulated differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) lysine degradation pathway and phenylalanine metabolism, and the downregulated DEGs were enriched in cutin, suberine, and wax biosynthesis. Furthermore, 44 DEGs were involved in pollen and anther development, such as tapetum, microspores, and pollen wall development. All of them were upregulated except a few such as POE1 genes (which encode Pollen Ole e I allergen and extensin family proteins). There were 261 specifically expressed DEGs (9 and 252 in sterile and fertile buds, respectively). Regarding the fertile bud-specific upregulated DEGs, the ubiquitin–proteasome pathway was enriched. The top four hub genes in the protein–protein interaction network (BnaA09g56400D, BnaA10g18210D, BnaA10g18220D, and BnaC09g41740D) encode RAD23d proteins, which deliver ubiquitinated substrates to the 26S proteasome. These findings provide evidence on the pathways regulated by Rfw and improve our understanding of fertility restoration.

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Section: Resultsmentioning

confidence: 99%

Section: Go and Kegg Analyses Of Degsmentioning

confidence: 99%

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Yang

Nong

et al. 2021

Front. Plant Sci.

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“…Except PLs and PGs, PMEs are also important for pollen development in plants. Recently, a CRISPR/Cas9 system-induced BcPME37c mutant has been characterized, and its mutation caused the abnormal thickening of the pollen intine in B. campestris (Xiong et al, 2019). The FIGURE 7 | Effect of HT on male fertility in WT, 35S:GmHSFA2 and 35S:GmHSP20a lines.…”

Section: Abnormal Anther/pollen Development Is Related To Male Fertilmentioning

confidence: 99%

Comparative Transcriptomics Analysis and Functional Study Reveal Important Role of High-Temperature Stress Response Gene GmHSFA2 During Flower Bud Development of CMS-Based F1 in Soybean

Ding

Guo

et al. 2020

Front. Plant Sci.

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High-temperature (HT) is one of the most important environmental factors that negatively impact the yield of some soybean cytoplasmic male sterility (CMS)-based hybrid (F1) combinations. The response of soybean to HT, especially at the male organ development stage, is poorly understood. To investigate the molecular mechanisms of the response from soybean CMS-based F1 male organ to HT, a detailed transcriptomics analysis was performed during flower bud development of soybean HT-tolerant and HT-sensitive CMS-based F1 combinations (NF1 and YF1) under normal-temperature and HT conditions. Obvious HT damage was observed by subjecting YF1 with HT, such as indehiscent anthers and decreased pollen fertility, whereas the male fertility of NF1 was normal. In total, 8,784 differentially expressed genes (DEGs) were found to respond to HT stress, which were mainly associated with anther/pollen wall development, carbohydrate metabolism and sugar transport, and auxin signaling. The quantitative real-time PCR (qRT-PCR) analysis and substance content detection also revealed that HT caused male fertility defects in YF1 by altering pectin metabolism, auxin, and sugar signaling pathways. Most importantly, the sugar signaling-PIF-auxin signaling pathway may underlie the instability of male fertility in YF1 under HT. Furthermore, HT induced the expression of heat shock factor (HSF) and heat shock protein (HSP) gene families. Overexpression of GmHSFA2 in Arabidopsis can promote the expression of HT protective genes (such as HSP20) by binding to the HSE motifs in their promoters, so as to improve the HT tolerance during flowering. Our results indicated that GmHSFA2 acted as a positive regulator, conferring HT tolerance improvement in soybean CMS-based F1. GmHSFA2 may be directly involved in the activation of male fertility protection mechanism in the soybean CMS-based F1 under HT stress.

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“…BcMF23a contributed to pollen development and pollen tube growth (Yue et al 2018a). BcPME37c in uenced pollen intine formation (Xiong et al 2019). However, studies on the biological function of PMEs, particularly in pollen development, still remain limited.…”

Section: Introductionmentioning

confidence: 99%

BcMF27, A Pectin Methylesterase Gene, Regulates Pollen Development and Pollen Tube Growth in Brassica Campestris

Yue

Cao

2021

Preprint

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Functional pollen grains are an essential ingredient of successful reproduction in flowering plants and are protected by outer walls. Pectin methylesterases (PMEs) modify pectin, a structural component of pollen intine. However, there are few studies on PMEs. Artificial microRNA (amiRNA) and overexpression technology was performed to investigate the function of pollen-specific PME gene, BcMF27, in pollen development. Knockdown of BcMF27 led to pollen wall collapse, 20% of which unknown material adhered to. Wall-collapsed pollen had abnormally thick intine outside of the germinal furrows. A portion of the cytoplasm was degraded in the remaining pollen with unknown material on the wall, in addition to a thick intine. Overexpression of BcMF27 resulted in 66.67% pollen wall disruption, causing an abnormally thick intine. In addition, functional interruption of BcMF27 gave rise to pollen tubes twisted in vitro. Taken together, BcMF27 contributes to the intine morphogenesis during pollen development and stabilizes pollen tube elongation. This research can promote knowledge of PMEs function and the molecular mechanism in pollen wall construction.

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BcPME37c is involved in pollen intine formation in Brassica campestris

Cited by 18 publications

References 32 publications

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Comparative Transcriptomics Analysis and Functional Study Reveal Important Role of High-Temperature Stress Response Gene GmHSFA2 During Flower Bud Development of CMS-Based F1 in Soybean

BcMF27, A Pectin Methylesterase Gene, Regulates Pollen Development and Pollen Tube Growth in Brassica Campestris

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