Abstract:Functional pollen grains are an essential ingredient of successful reproduction in flowering plants and are protected by outer walls. Pectin methylesterases (PMEs) modify pectin, a structural component of pollen intine. However, there are few studies on PMEs. Artificial microRNA (amiRNA) and overexpression technology was performed to investigate the function of pollen-specific PME gene, BcMF27, in pollen development. Knockdown of BcMF27 led to pollen wall collapse, 20% of which unknown material adhered to. Wal… Show more
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