Batch Cultures of Somatic Embryos of<i>Digitalis lanata</i>in Gaslift Fermenters. Development and Cardenolide Accumulation

Greidziak, N.; Diettrich, B.; Luckner, Martin

doi:10.1055/s-2006-960919

Cited by 25 publications

(9 citation statements)

References 8 publications

(8 reference statements)

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“…Stuhlemmer et al [6,60] and Greidziak et al [6,61] reported the formation of about 1 mg cardenolides/g dry weight in partly submerged shoot cultures and somatic embryoids, respectively. In a more recent study, salicylic acid, exogenous yeast polysaccharides, and calcium chloride were tested for their capacity to enhance cardenolide formation in D. lanata shoot cultures.…”

Section: Biology and Physiologymentioning

confidence: 99%

The Foxgloves (Digitalis) Revisited

Kreis

2017

Planta Med

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This review provides a renewed look at the genus Digitalis. Emphasis will be put on those issues that attracted the most attention or even went through paradigmatic changes since the turn of the millennium. PubMed and Google Scholar were used (“Digitalis” and “Foxglove” were the key words) to identify research from 2000 till 2017 containing data relevant enough to be presented here. Intriguing new results emerged from studies related to the phylogeny and taxonomy of the genus as well as to the biosynthesis and potential medicinal uses of the key active compounds, the cardiac glycosides. Several Eastern and Western Foxgloves were studied with respect to their propagation in vitro. In this context, molecular biology tools were applied and phytochemical analyses were conducted. Structure elucidation and analytical methods, which have experienced less exciting progress, will not be considered here in great detail.

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Section: Biology and Physiologymentioning

confidence: 99%

The Foxgloves (Digitalis) Revisited

Kreis

2017

Planta Med

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“…The large scale production of plants using bioreactors employing liquid medium can be achieved through induction of somatic embryogenesis, as in Digitalis lanata (Greidziak et al 1990), or by using shoot meristem multiplication technique as in Digitalis purpurea (Hagimori et al 1984) and in Stevia rebundiana (Akita et al 1994). Some other important medicinal plants propagated in bioreactors include: Atropa belladonna, Asparagus officinalis, Artemisa annua, Acanthopanax koreanum, Chlorophytum borivilianum, Digitalis lanata and Brahmi (Mehrotra et al 2007;Praveen 2009;Haque et al 2009;Rizvi and Kukreja 2014).…”

Section: Production Of Secondary Metabolites Of Maps From Cellmentioning

confidence: 99%

In Vitro Micropropagation of Medicinal and Aromatic Plants

Máthé

Hassan

Kader

2015

Medicinal and Aromatic Plants of the World

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Modern biotechnological methods like in vitro micropropagation technique hold tremendous potential for the production of high-quality plant-based medicine. They also allow to achieve the large scale multiplication of diseasefree plants, faster cloning and the conservation of desired genotypes, in a very short span of time. Via genetic transformation techniques, the modification of both genetic information of MAPs and the regulation of genes responsible for the production of valuable biologically active substances has also become possible in either higher amounts or with better properties.Micropropagation protocols are worked out for many plant species cultured in vitro to provide macro-and micro-mineral nutrients, vitamins, source of carbohydrates, appropriate environmental conditions (light intensity, photoperiod and temperature) and plant growth regulators required to obtain high regeneration rates. As such they are expected to facilitate commercially feasible micropropagation. Well-defined cell culture methods have also been developed to produce pharmacologically important secondary metabolites. Genetic engineering is applied to produce transgenic medicinal plants and metabolites.This chapter offers a brief insight into the present status of the biotechnology of MAPs. Special emphasis has been placed on the in vitro micropropagation and rapid clonal multiplication of selected elite genotypes, the regulation of organogenesis and somatic embryogenesis, the exploitation of advantages in somaclonal variation and genetic engineering techniques for both crop improvement and in vitro germplasm conservation. The production of flavor and volatile constituents in tissue cultures will also be briefly discussed, similarly to the application of farther biotechnological approaches.

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“…Some examples of this effect are the synthesis of tropane alkaloids in roots differentiated from callus of Atropa belladonna,lo the production of morphinane alkaloids during somatic embryogenesis of Papaver somniferum, II the synthesis of indole alkaloids in root and shoot cultures of C. roseus,12 the synthesis of the flavonoid naringin in shoot cultures of Citrus paradisi, 13 and the accumulation of cardenolides in somatic embryos of Digitalis lanata. 14 The expression of secondary metabolic pathways in redifferentiated cell cultures is actually not surprising because it mimics exactly what the plant does. Furthermore, in most cases the synthesis of plant natural products is under strict temporal and spatial control.…”

Section: Introductionmentioning

confidence: 99%