Root Culture as a Source of Secondary Metabolites of Economic Importance

Loyola‐Vargas, Víctor M.; Miranda-Ham, María de Lourdes

doi:10.1007/978-1-4899-1778-2_10

Cited by 11 publications

(3 citation statements)

References 160 publications

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“…The growth and stability of root cultures can be improved if they are transformed by Agrobacterium rhizogenes. Hairy root cultures are the result (reviews: Flores et al, 1987;Hamill et al, 1987;Loyola-Vargas and Miranda-Ham, 1995;Wysokinska and Chmiel, 1997;Giri and Narasu, 2000;Bourgaud et al, 2001;Kim et al, 2002;Ramachandra and Ravishankar, 2002;Sevon and Oksman-Caldentey, 2002;Verpoorte et al, 2002). Bioreactors have also been developed for shoot and root cultures that allow the large-scale production of plant material (Doran, 1997 Jeong et al, 2003;Palazon et al, 2003;Wildi et al, 2003;Hvoslef-Eide and Preil, 2004;Ramakrishnan and Curtis, 2004).…”

Section: Sustainable Production Of Anticancer Compounds By In Vitro Cmentioning

confidence: 99%

“…anthocyanins, betalains, phenolics, rosmarinic acid, taxol, anthraquinones, berberine, nicotine) whereas these culture types mostly failed to produce economically important substances (such as vincristine, morphine or scopolamine). On the other hand, organized tissue cultures hardly ever fail to synthesize secondary metabolites (reviews: Flores et al, 1987;Hamill et al, 1987;Wink, 1987a, b;Loyola-Vargas and Miranda-Ham, 1995;Doran, 1997;Wysokinska and Chmiel, 1997;Giri and Narasu, 2000;Bourgaud et al, 2001;Kim et al, 2002;Ramachandra and Ravishankar, 2002;Sevon and Oksman-Caldentey, 2002;Verpoorte et al, 2002). Their capacity obviously depends on the site of synthesis in the intact plant.…”

Section: Sustainable Production Of Anticancer Compounds By In Vitro Cmentioning

confidence: 99%

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Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents

Wink

Alfermann

Franke

et al. 2005

Plant Genet. Resour.

146

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Due to their complex structure with several chiral centres important anticancer agents are still extracted from plants and not synthesized chemically on a commercial scale. Sustainable bioproduction of the compounds of interest may be achieved by plant in vitro cultures. Undifferentiated callus and suspension cultures, which can be cultivated in large bioreactors easily, very often fail to accumulate the compounds of interest, whereas shoot and root cultures as well hairy roots normally produce the same compounds as in the appropriate organs. The production of anticancer compounds, such as the alkaloids vinblastine, vincristine, paclitaxel (Taxol®), camptothecin, or the lignan podophyllotoxin, by plant in vitro cultures is reviewed. Taxanes can be produced in bioreactors using cell suspensions of various Taxus species with good yields; presently paclitaxel is produced on a commercial scale by Phyton Biotech (Germany). Camptothecin has low yields in suspension cultures of Camptotheca acuminata or Nothapodytes foetida (0.0003–0.01%), but a good production (0.1–0.3% dry wt) in root and hairy root cultures of Ophiorrhiza pumila, O. mungos and C. acuminata. Podophyllotoxin can be produced in cell suspension and root as well as hairy root cultures of Podophyllum and various Linum species up to 130 mg/l (Linum album cell suspensions); its derivative 6-methoxypodophyllotoxin is accumulated in hairy roots of L. persicum up to about 500 mg/l. The in vitro production of dimeric indole alkaloids in Catharanthus roseus has failed so far both in undifferentiated and differentiated in vitro cultures. In cases where in vitro cultures show good yields, they can be employed in biotechnology for the sustainable production of valuable products.

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Section: Sustainable Production Of Anticancer Compounds By In Vitro Cmentioning

confidence: 99%

Section: Sustainable Production Of Anticancer Compounds By In Vitro Cmentioning

confidence: 99%

Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents

Wink

Alfermann

Franke

et al. 2005

Plant Genet. Resour.

146

View full text Add to dashboard Cite

show abstract

“…In the past, the first system enabling the elongation and ramification of root tips was a medium containing mineral nutrient elements, sugar, and vitamins. Thereafter, root culture became an experimental model used to study mineral nutrition, growth regulation, and root development (Loyola-Vargas and Miranda-Ham, 1995). This technique was used for the current study, in association with an original method of continuous measurement under sterile conditions of the consumption of mineral nutrient elements during root growth.…”

Section: Mineral Nutrition Of Gypsophila In Vitro Root Culturementioning

confidence: 99%

Mineral nutrition ofGypsophila in vitroroot culture

Morard

Fulcheri

Henry³

1999

Journal of Plant Nutrition

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Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9

Loyola‐Vargas

Avilez-Montalvo

2018

Methods in Molecular Biology

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Plant tissue culture (PTC) is a set of techniques for culturing cells, tissues, or organs in an aseptic medium with a defined chemical composition, in a controlled environment. Tissue culture, when combined with molecular biology techniques, becomes a powerful tool for the study of metabolic pathways, elucidation of cellular processes, genetic improvement and, through genetic engineering, the generation of cell lines resistant to biotic and abiotic stress, obtaining improved plants of agronomic interest, or studying the complex cellular genome. In this chapter, we analyze in general the use of plant tissue culture, in particular protoplasts and calli, in the implementation of CRISPR/Cas9 technology.

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Root Culture as a Source of Secondary Metabolites of Economic Importance

Cited by 11 publications

References 160 publications

Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents

Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents

Mineral nutrition ofGypsophila in vitroroot culture

Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9

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