Basic physico-chemical parameters of milt from sea trout (Salmo truttam.trutta), brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss)

Abstract

The aim of the study was to compare the physico-chemical parameters of milt from sea trout (Salmo trutta m. trutta), brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). Milt was collected by stripping and spermatozoa concentrations, were determined and compared with sperm motility and spermatocrit values along with seminal plasma indices (pH, osmolality, sodium, potassium, chlorine, calcium, magnesium, glucose and protein concentrations). The highest spermatozoa concentration of 22.3 ± 6.7 · 10 9 ml )1 was found in the sea trout milt, and was significantly different of those observed in brook trout (11.9 ± 3.3 · 10 9 ml )1 ) and rainbow trout (10.7 ± 4.4 · 10 9 ml )1 ). The values for pH and K + did not differ significantly among species. The mean pH was 8.0 in the milt of each species and the K + concentrations ranged from 24.8 ± 7.2 to 30.5 ± 7.6 mM M L )1 . Considerable differences were determined for the Ca 2+ ions concentrations. The highest value was found in sea trout (1.7 ± 0.3 mM M L )1 ), while in the rainbow trout it was 0.7 ± 0.5 and in the brook trout 0.4 ± 0.1 mM M L )1 . The most pronounced differences were found in the glucose concentration cause of its unnaturally low concentration in rainbow trout of the mean value of 6.0 ± 15.2 mg L )1 . The mean value in sea trout and brook trout was 185.0 ± 172.4 and 231.2 ± 148.4 mg L )1 respectively. For all species, protein mean values were below 1.3 g L )1 . The mean osmolality was between 230.6 ± 98.6 and 272.0 ± 26.4 mOsm kg )1 in the species studied. No correlation was found between any components determined in milt and the spermatozoa motility (P > 0.05). The sperm concentration was positively correlated with the protein content in the milt of the three species studied, other less exhibited correlation was found.

Hatchery performance in a major rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) seed production area of Turkey

“…Indeed, the number of male fish accounted for only 22.6% of the total number broodstock in the region (Kanyılmaz et al., ). However, sperm concentration and pH values in the farms are either similar to or higher than those reported in the literature (Lahnsteiner and Patzner, ; Tekin et al., , ; Dietrich et al., ; Bozkurt, ; Canyurt and Akhan, ,b; Dziewulska et al., ; Özgür and Bayır, ).…”

“…Indeed, the number of male fish accounted for only 22.6% of the total number broodstock in the region (Kanyılmaz et al, 2011). However, sperm concentration and pH values in the farms are either similar to or higher than those reported in the literature (Lahnsteiner and Patzner, 2002;Tekin et al, 2003Tekin et al, , 2007Dietrich et al, 2005;Bozkurt, 2006;Canyurt and Akhan, 2008a,b;Dziewulska et al, 2008;€ Ozg€ ur and Bayır, 2013). The duration of motility values were comparable among farms, but motility percentages were higher in farms A and F, which used lower water temperatures than the other farms.…”

Properties and activities of blood- or seawater-contaminated wild-caught Striped Jewfish (Stereolepis doederleini) sperm

“…The spermatocrit (79 ± 3%) of striped jewfish was higher than reported in other species (Aas, Refstie, & Gjerde, ; Cruz‐Casallas, Lombo‐Rodríguez, & Velasco‐Santamaría, ; Dziewulska, Rzemieniecki, & Domagala, ; Le, Lim, Min, Kim, & Chang, ; Lim et al., , ; Suquet, Omnes, Normant, & Fauvel, ) and lower than that of Grey mullet Mugil cephalus (Chang, Choi, Lim, & Kho, ) and other flat fish species (Chang & Chang, ). The spermatocrit of semen also varied between conspecific males, species and across the reproductive season (Rakitin, Ferguson, & Trippel, ; Rurangwa et al., ).…”

“…Sodium, potassium and chloride ion concentrations were previously reported to be 164, 9.8 and 151 mmol/L in Filefish ( Thamnaconus modestus ) semen (Le et al., ), 155, 5.4 and 99 mmol/L in Starry flounder, Platichthys stellatus (Lim et al., ) and 131, 10.7, 106.8 mmol/L in European perch, Perca fluviatilis (Alavi et al., ) respectively. The calcium and magnesium levels of Striped jewfish seminal plasma were higher and lower (Dziewulska et al., ; Le et al., ; Lim et al., , ) than previously reported in other fish species. The osmolality value of seminal plasma in Striped jewfish was higher, compared with European perch; 298.1 mOsm/kg (Alavi et al., ), in Burbot ( Lota lota) ; 290.1 mOsm/kg (Lahnsteiner, Berger, Weismann, & Patzner, ) and Jundiá ( Rhamdia quelen) ; 274.8 mOsm/kg (Borges et al., ).…”

Effect of blood plasma on motility of steelhead sperm

“… † Morisawa et al (1983); Koldras et al (1996); Lahnsteiner, Berger, Weismann and Patzner (1998); Glogowski, Kwasnik, Piros, Drabowski, Goryczko, Dobosz, Kuzminski and Ciereszko (2000); Dziewulska, Rzemieniecki and Domagala (2008). …”