Effect of blood plasma on motility of steelhead sperm

Ingermann, Rolf L.; Kanuga, M.K.; Wilson-Leedy, Jonas G.

doi:10.1111/j.1365-2109.2009.02398.x

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…The number of frames, video quality, microscopy objectives, and number of evaluated images are crucial for performing the analysis. Different settings on the CASA plugin dialogue box were also established for other Brazilian and temperate species and adjusted according to the sperm characteristics (Wilson‐Leedy and Ingermann, ; Ingermann et al., ; Sanches et al., ; Bombardelli et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…The use of the ImageJ for sperm parameter evaluation was efficient for fish species such as: Danio rerio (Wilson‐Leedy and Ingermann, ; Wilson‐Leedy et al., ; Ingermann et al., ; Kanuga et al., ); Oncorhynchus mykiss (Ingermann et al., ); Pimephales promelas, (Murack et al., ); Rhamdia quelen (Sanches et al., ); and Steindachneridion melanodermatum (Bombardelli et al., ). Besides fish, the method was also applied to the blue mussels Mytilus edulis and M. trossulus (Liu et al., ), and the horse sparrow Passer domesticus (Helfenstein et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Sperm motility parameters forSteindachneridion parahybaebased on open-source software

et al. 2013

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Summary The objective of this work was to evaluate the sperm motility of 13 Steindachneridion parahybae males using open‐source software (ImageJ/CASA plugin). The sperm activation procedure and image capture were initiated after semen collection. Four experimental phases were defined from the videos captured of each male as follows: (i) standardization of a dialogue box generated by the CASA plugin within ImageJ; (ii) frame numbers used to perform the analysis; (iii) post‐activation motility between 10 and 20 s with analysis at each 1 s; and (iv) post‐activation motility between 10 and 50 s with analysis at each 10 s. The settings used in the CASA dialogue box were satisfactory, and the results were consistent. These analyses should be performed using 50 frames immediately after sperm activation because spermatozoa quickly lose their vigor. At 10 s post‐activation, 89.1% motile sperm was observed with 107.2 μm s−1 curvilinear velocity, 83.6 μm s−1 average path velocity, 77.1 μm s−1 straight line velocity; 91.6% were of straightness and 77.1% of wobble. The CASA plugin within ImageJ can be applied in sperm analysis of the study species by using the established settings.

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Section: Discussionmentioning

confidence: 99%