Baicalin prevents LPS-induced activation of TLR4/NF-κB p65 pathway and inflammation in mice via inhibiting the expression of CD14

Fu, Yajun; Xu, Bo; Huang, Shaowei; Luo, Xia; Deng, Xiangliang; Luo, Shuang; Liu, Chang; Wang, Qing; Chen, Jinyan; Zhou, Lian

doi:10.1038/s41401-020-0411-9

Cited by 88 publications

(53 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Baicalin can significantly reduce the serum interleukin-17 (IL-17), IL-6, and IL-1β expression in Ulcerative Colitis (UC) rats (55). In addition, it can also reduce the expression of CD14 and IL-6 in colonic mucosa and alleviate the severity of ulcers (56). The results of these studies have shown that baicalin treatment can inhibit the inflammatory symptoms of the intestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Baicalin–Zinc Complex Alleviates Inflammatory Responses and Hormone Profiles by Microbiome in Deoxynivalenol Induced Piglets

Zha

Cui

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

This study aimed to investigate the beneficial effect of baicalin–zinc complex (BZN) on intestinal microorganisms in deoxynivalenol (DON)-challenged piglets and the association between intestinal microorganisms and host immunity and hormone secretion. Forty weaned piglets were randomly divided into four treatments with 10 piglets in each treatment: (1) control (Con) group (pigs fed basal diet); (2) DON group (pigs fed 4 mg DON/kg basal diet); (3) BZN group (pigs fed 0.5% BZN basal diet); and (4) DBZN group (pigs fed 4 mg DON/kg and 0.5% BZN basal diet). The experiment lasted for 14 days. The BZN supplementation in DON-contaminated diets changed the intestinal microbiota composition and increased intestinal microbial richness and diversity of piglets. The BZN supplementation in DON-contaminated diets also alleviated the inflammatory responses of piglets and modulated the secretion of hormones related to the growth axis. Moreover, microbiota composition was associated with inflammatory and hormone secretion. In conclusion, BZN alleviated inflammatory response and hormone secretion in piglets, which is associated with the intestinal microbiome.

show abstract

Section: Discussionmentioning

confidence: 99%

Baicalin–Zinc Complex Alleviates Inflammatory Responses and Hormone Profiles by Microbiome in Deoxynivalenol Induced Piglets

Zha

Cui

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…This prescription contains a total of five herbs: Huang Qin (Scutellariae Radix), Ku Shen (Sophorae Flavescentis Radix), Fang Feng (Saposhnikoviae Radix), Chen Pi (Citrus Reticulata) and Xi Yang Shen (Panacis Quinquefolii Radix). Modern pharmacological studies indicated that Scutellariae Radix and Saposhnikoviae Radix in GXF have been reported to attenuate inflammation [ 11 , 12 ]. In addition, Sophorae Flavescentis Radix has also been reported to have anti-fibrotic effects [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Revealing potential anti-fibrotic mechanism of Ganxianfang formula based on RNA sequence

Liu

Xiang

et al. 2022

Chin Med

View full text Add to dashboard Cite

Background Ganxianfang (GXF) formula as a traditional Chinese medicine (TCM) is used for liver fibrosis in clinical practice while its mechanism is unclear. The aim of this study is to explore the molecular mechanism of GXF against CCl4-induced liver fibrosis rats. Methods Detected the main compounds of GXF by UPLC-MS/MS. Evaluated the efficacy of GXF (1.58, 3.15, 4.73 g/kg/day) and Fuzheng Huayu (FZHY, positive control, 0.47 g/kg/day) through serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels and histopathological changes. Explored the underlying mechanisms by integrating our total liver RNA sequencing (RNA-seq) data with recent liver single-cell sequencing (scRNA-seq) studies. Verified potential pharmacodynamic substances of GXF by hepatic stellate cell (HSC)-T6 line. Results Main compounds were identified in GXF by UPLC-MS/MS, including baicalin, wogonoside and matrine etc. With GXF-high dose treatment, the elevation of ALT and AST induced by CCl4 were significantly reduced, and the protective effect of GXF-high dose treatment was better than FZHY. Liver histopathological changes were alleviated by GXF-high dose treatment, the ISHAK scoring showed the incidence of liver cirrhosis (F5/F6) decreased from 76.5 to 55.6%. The results of liver hydroxyproline content were consistent with the histopathological changes. RNA-seq analysis revealed the differential genes (DEGs) were mainly enriched in ECM-receptor interaction and chemokine signaling pathway. GXF effectively inhibited collagen deposition and significantly downregulated CCL2 to inhibit the recruitment of macrophages in liver tissue. Integrating scRNA-seq data revealed that GXF effectively inhibited the expansion of scar-associated Trem2+CD9+ macrophages subpopulation and PDGFRα+PDGFRβ+ scar-producing myofibroblasts in the damaged liver, and remodeled the fibrotic niche via regulation of ligand-receptor interactions including TGFβ/EGFR, PDGFB/PDGFRα, and TNFSF12/TNFRSF12a signaling. In vitro experiments demonstrated that baicalin, matrine and hesperidin in GXF inhibited the activation of hepatic stellate cells. Conclusions This study clarified the potential anti-fibrotic effects and molecular mechanism of GXF in CCl4-induced liver fibrosis rats, which deserves further promotion and application.

show abstract

“…Baicalin, which is isolated from the Scutellaria baicalensis Georgi (Huang Qin), has been clinically utilized for centuries as a traditional Chinese herbal medicine in China. Baicalin was shown to display various biological properties, including antioxidative, anti-proliferative, anti-inflammatory, antitumor activities, and protective effects against multiple-tissue or organ damage (Chen et al, 2014;Fu et al, 2020;Liu et al, 2020;Song et al, 2020). Recently, few studies have revealed the antitumor action of baicalin in lung cancer cells (Wei et al, 2017;Diao et al, 2019); however, the therapeutic effects and underlying mechanism of baicalin in lung cancer have not been elucidated yet.…”

Section: Introductionmentioning

confidence: 99%

Baicalin Induces Apoptosis and Suppresses the Cell Cycle Progression of Lung Cancer Cells Through Downregulating Akt/mTOR Signaling Pathway

Sui

Han

Chen

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

Baicalin, as a natural active ingredient extracted and isolated from the traditional Chinese medicine Scutellaria baicalensis Georgi., has been potentially used in various areas for its antioxidative, antitumor, anti-inflammatory, and anti-proliferative activities. Although several studies have reported the antitumor effects of baicalin against various cancer types, its beneficial effects on lung cancer have not yet been elucidated. Therefore, the therapeutic effects and molecular mechanisms of baicalin on lung cancer cell lines H1299 and H1650 were investigated. Here, the results of its antitumor activity were shown. We found that Akt/mTOR pathway inhibition was the essential determinant in baicalin-induced cell cycle arrest. Furthermore, when the Akt Agonist SC79 or Akt plasmid transfection was performed, the antitumor effect of baicalin was significantly abrogated in both H1299 and H1650 cells. In conclusion, we found that baicalin exerted its antitumor activity mainly by inducing Akt-dependent cell cycle arrest and promoting apoptosis, which show great potential for developing a new drug for lung cancer treatment.

show abstract

Baicalin prevents LPS-induced activation of TLR4/NF-κB p65 pathway and inflammation in mice via inhibiting the expression of CD14

Cited by 88 publications

References 27 publications

Baicalin–Zinc Complex Alleviates Inflammatory Responses and Hormone Profiles by Microbiome in Deoxynivalenol Induced Piglets

Baicalin–Zinc Complex Alleviates Inflammatory Responses and Hormone Profiles by Microbiome in Deoxynivalenol Induced Piglets

Revealing potential anti-fibrotic mechanism of Ganxianfang formula based on RNA sequence

Baicalin Induces Apoptosis and Suppresses the Cell Cycle Progression of Lung Cancer Cells Through Downregulating Akt/mTOR Signaling Pathway

Contact Info

Product

Resources

About