Baicalin induces apoptosis and autophagy in human osteosarcoma cells by increasing ROS to inhibit PI3K/Akt/mTOR, ERK1/2 and β-catenin signaling pathways

Pang, He; Wu, Tingrui; Peng, Zhonghua; Tan, Qichao; Peng, Xin; Zhan, Zeyu; Song, Lijun; Wei, Bo

doi:10.1016/j.jbo.2022.100415

Cited by 32 publications

(23 citation statements)

References 75 publications

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“…The reason why overexpression of lncRNA HOTAIR had no significant effect on the expression of ADIPOR2 may be that ADIPOR1 is expressed abundantly in cartilages, bones, and synovial tissues, whereas ADIPOR2 is rarely detected [ 44 ]. Notably, baicalin as a natural flavonoid glycoside with significant anti-inflammation activity can inhibit the PI3K/AKT signaling pathway to induce the apoptosis and autophagy of human osteosarcoma cells [ 45 ]. Similarly, our subsequent cell experiments also confirmed that baicalin and lncRNA HOTAIR silencing exerted synergistic effects on the PTEN/PI3K/AKT signaling pathway inflammatory factors.…”

Section: Discussionmentioning

confidence: 99%

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

et al. 2023

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Objective This study aims to investigate the correlation of long non-coding RNA HOX transcript antisense RNA (lncRNA HOTAIR) with the PTEN/PI3K/AKT pathway and clinical-related indicators in osteoarthritis (OA) and determine the effect of baicalin intervention. Methods The levels of clinical lipid metabolism indexes and immune-inflammatory indexes in OA patients and normal controls was detected. OA chondrocytes (OA-CHs) were induced with peripheral blood mononuclear cells (PBMCs), followed by baicalin treatment (50 ug/mL). RT-qPCR was performed to measure lncRNA HOTAIR expression. The levels of inflammatory cytokines and adiponectin were detected using ELISA kits. CCK-8 assay was used to assess the viability of CHs. The related protein expression was measured using Western blot analysis. Results LncRNA HOTAIR might act as a biomarker of OA in vivo. LncRNA HOTAIR was positively correlated with TC, hs-CRP, IgA, TNF-α, and VAS score. Overexpression of lncRNA HOTAIR in vitro inhibited cell proliferation, reduced IL-10 and PTEN expression, but augmented TNF-α, p-PI3K, and p-AKT proteins in OA-CHs stimulated by OA-PBMCs. The changes of above indexes were also observed in OA-CHs stimulated by OA-PBMCs treated with si-lncRNA HOTAIR or baicalin, implying the synergistic effects of baicalin and lncRNA HOTAIR silencing on OA. Conclusions Conclusively, lncRNA HOTAIR was highly expressed in OA-CHs, which facilitated OA inflammatory responses by orchestrating inflammatory cytokines and the PTEN/PI3K/AKT pathway. Baicalin exerted therapeutic effects by inhibiting the expression of lncRNA HOTAIR, decreasing the protein levels of p-PI3K and p-AKT, and increasing the protein levels of PTEN, APN, and ADIPOR1.

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Section: Discussionmentioning

confidence: 99%

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

et al. 2023

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“…Nowadays, it has been demonstrated that the growth of various cancers, such as lung, breast, bladder, and colon cancers, could also be suppressed by baicalin [ 223 ]. For example, baicalin was able to induce ROS accumulation to inhibit the PI3K/AKT/mTOR signaling, thereby triggering cytotoxic autophagy in human osteosarcoma cells [ 136 ]. In another study, baicalin was loaded with folic acid-modified albumin nanoparticles (FA-BSANPs/BA) to improve its biocompatibility and prolong its circulation time in vivo.…”

Section: Phytochemicals Targeting Ros and Autophagy For Cancer Therapymentioning

confidence: 99%

Targeting the Interplay of Autophagy and ROS for Cancer Therapy: An Updated Overview on Phytochemicals

Dong

Luo

et al. 2023

Pharmaceuticals

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Autophagy is an evolutionarily conserved self-degradation system that recycles cellular components and damaged organelles, which is critical for the maintenance of cellular homeostasis. Intracellular reactive oxygen species (ROS) are short-lived molecules containing unpaired electrons that are formed by the partial reduction of molecular oxygen. It is widely known that autophagy and ROS can regulate each other to influence the progression of cancer. Recently, due to the wide potent anti-cancer effects with minimal side effects, phytochemicals, especially those that can modulate ROS and autophagy, have attracted great interest of researchers. In this review, we afford an overview of the complex regulatory relationship between autophagy and ROS in cancer, with an emphasis on phytochemicals that regulate ROS and autophagy for cancer therapy. We also discuss the effects of ROS/autophagy inhibitors on the anti-cancer effects of phytochemicals, and the challenges associated with harnessing the regulation potential on ROS and autophagy of phytochemicals for cancer therapy.

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“…Chelation of free Ca 2+ with BAPTA-AM also downregulated apoptosis induction and ROS accumulation. Consequently, baicalin may be observed as a viable contender for the diagnosis of osteosarcoma [ 140 ].…”

Section: Regulation Of Autophagy By Scutellaria Fl...mentioning

confidence: 99%

Autophagy Induction by Scutellaria Flavones in Cancer: Recent Advances

Tuli

Bhushan

Kumar³

et al. 2023

Pharmaceuticals

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In parallel with a steady rise in cancer incidence worldwide, the scientific community is increasingly focused on finding novel, safer and more efficient modalities for managing this disease. Over the past decades, natural products have been described as a significant source of new structural leads for novel drug candidates. Scutellaria root is one of the most studied natural products because of its anticancer potential. Besides just describing the cytotoxic properties of plant constituents, their molecular mechanisms of action in different cancer types are equally important. Therefore, this review article focuses on the role of the Scutellaria flavones wogonin, baicalein, baicalin, scutellarein and scutellarin in regulating the autophagic machinery in diverse cancer models, highlighting these molecules as potential lead compounds for the fight against malignant neoplasms. The knowledge that autophagy can function as a dual-edged sword, acting in both a pro- and antitumorigenic manner, further complicates the issue, revealing an amazing property of flavonoids that behave either as anti- or proautophagic agents.

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Baicalin induces apoptosis and autophagy in human osteosarcoma cells by increasing ROS to inhibit PI3K/Akt/mTOR, ERK1/2 and β-catenin signaling pathways

Cited by 32 publications

References 75 publications

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

Targeting the Interplay of Autophagy and ROS for Cancer Therapy: An Updated Overview on Phytochemicals

Autophagy Induction by Scutellaria Flavones in Cancer: Recent Advances

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