Bacteriorhodopsin Thin-Film Assemblies—Immobilization, Properties, and Applications

He, Jin‐An; Samuelson, Lynne A.; Li, Lian; Kumar, Jayant; Tripathy, Sukant K.

doi:10.1002/(sici)1521-4095(199904)11:6<435::aid-adma435>3.0.co;2-u

Cited by 93 publications

(63 citation statements)

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“…The thickness of bR was measured before and after OPV binding using atomic force microscopy. As displayed in Figure S5 (Supporting Information), the height of bR was ≈5.0 nm that was consistent with the reported data . After OPV binding, the thickness of PM fragments increased to ≈5.4 nm, which further verified the binding of OPV on bR.…”

Section: Methodssupporting

confidence: 90%

“…The bR embedded in PM fragments was isolated from Halobacterium salinarum . It exhibited negative charge properties on both sides of the membranes due to the phosphate groups of phospholipids and the amino acid residues on surface . The cationic OPV was anticipated to bind onto bR, which was verified by confocal laser scanning microscopy (CLSM).…”

Section: Methodsmentioning

confidence: 86%

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Bacteriorhodopsin‐Based Biophotovoltaic Devices Driven by Chemiluminescence as Endogenous Light Source

Zhou

Liu

et al. 2019

Advanced Optical Materials

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As a light‐driven proton pump, bacteriorhodopsin (bR) captures light and transports protons directionally. Here, a new bR‐based bioelectronic device that employs the chemiluminescence from luminol as an endogenous light source to generate steady current is designed. Purple membrane embedded with bR is modified on indium tin oxide (ITO) electrode followed by electrostatically binding of cationic oligo (p‐phenylene vinylene) derivative (OPV). The device is fabricated by coupling bR/OPV electrode and bare ITO electrode. As a suitable luminescence acceptor of luminol, OPV emits green light through chemiluminescence resonance energy transfer (CRET), which is capable of exciting ground state bR (bR570) nearby to initiate photocycle. Simultaneously, luminol emits blue chemiluminescent light which can be absorbed by M410 intermediate in photocycle, accelerating its reconversion to bR570 by shortcuts. As a result, the photocycle is accelerated and stabilized to generate a stable photocurrent. Furthermore, this study achieves to use oxygen and glucose as the initial source of hydrogen peroxide and produce stable photocurrent by taking advantage of the cascade reaction of glucose oxidase and horseradish peroxide. This provides a proof‐of‐concept for successfully using the CRET system as endogenous light source and provides new ideas for energy utilization and conversion taking advantage of natural bR‐based photosynthetic systems.

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Section: Methodssupporting

confidence: 90%

Section: Methodsmentioning

confidence: 86%

Bacteriorhodopsin‐Based Biophotovoltaic Devices Driven by Chemiluminescence as Endogenous Light Source

Zhou

Liu

et al. 2019

Advanced Optical Materials

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“…This provides an easy method for aligning and depositing the PM patches for sensor applications. The charge density, and therefore the electronegativity, can be changed by changing the pH of the suspension carrying the PM [6,7]. The presence and the ability to change the electrical characteristic are important for orienting and depositing PM by electrodeposition, Langmuir-Blodgett, or ionic self-assembly techniques.…”

Section: Optoelectric Bacteriorhodopsinmentioning

confidence: 99%

“…This not only orients the patches but also causes them to deposit on one of the electrodes with the more negatively charged cytoplasmic side toward the positive electrode [7]. While the method is easy and quick, there is no control over the number of layers deposited.…”

Section: Optoelectric Bacteriorhodopsinmentioning

confidence: 99%

Recent Advances in the Field of Bionanotechnology: An Insight into Optoelectric Bacteriorhodopsin, Quantum Dots, and Noble Metal Nanoclusters

Knoblauch

Griep

Friedrich

2014

Sensors

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Molecular sensors and molecular electronics are a major component of a recent research area known as bionanotechnology, which merges biology with nanotechnology. This new class of biosensors and bioelectronics has been a subject of intense research over the past decade and has found application in a wide variety of fields. The unique characteristics of these biomolecular transduction systems has been utilized in applications ranging from solar cells and single-electron transistors (SETs) to fluorescent sensors capable of sensitive and selective detection of a wide variety of targets, both organic and inorganic. This review will discuss three major systems in the area of molecular sensors and electronics and their application in unique technological innovations. Firstly, the synthesis of optoelectric bacteriorhodopsin (bR) and its application in the field of molecular sensors and electronics will be discussed. Next, this article will discuss recent advances in the synthesis and application of semiconductor quantum dots (QDs). Finally, this article will conclude with a review of the new and exciting field of noble metal nanoclusters and their application in the creation of a new class of fluorescent sensors.

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“…6,7 In some microorganisms, light-activated proton pumps, such as bacteriorhodopsin (bR) with retinal compounds serving as chromophores, change their conformations rapidly after photons absorption to uptake and release protons across the plasma membranes, which contributes to the generation of bioelectricity. [8][9][10][11] Inspired by these natural processes, creating artificial light-harvesting systems to form electrochemical gradient is particularly attractive because of their promising applications for photoelectric conversion. In recent years, such light-harvesting systems have been constructed by the combination of polymer porous membranes and photosensitive molecules, such as photo-acid, 12 malachite green, 13 and spiropyran 14,15 .…”

mentioning

confidence: 99%

Photocurrent generation in a light-harvesting system with multifunctional artificial nanochannels

Zhang

Liu

2015

Chem. Commun.

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We develop an artificial light-harvesting system for photocurrent generation. Platinized TiO2 nanochannels are used as ultraviolet-light-harvesting antennae, which generate a transmembrane electrochemical gradient by asymmetric work function driven photochemical reactions. As a result, a potential difference is formed across the nanochannels, producing a photocurrent flowing through the external circuit.

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Bacteriorhodopsin Thin-Film Assemblies—Immobilization, Properties, and Applications

Cited by 93 publications

References 0 publications

Bacteriorhodopsin‐Based Biophotovoltaic Devices Driven by Chemiluminescence as Endogenous Light Source

Bacteriorhodopsin‐Based Biophotovoltaic Devices Driven by Chemiluminescence as Endogenous Light Source

Recent Advances in the Field of Bionanotechnology: An Insight into Optoelectric Bacteriorhodopsin, Quantum Dots, and Noble Metal Nanoclusters

Photocurrent generation in a light-harvesting system with multifunctional artificial nanochannels

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