Bacteriophages induced from lysogenic root canal isolates of <i>Enterococcus faecalis</i>

Stevens, R. H.; Porras, Omar; Delisle, Allan L.

doi:10.1111/j.1399-302x.2009.00506.x

Cited by 42 publications

(49 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TUSoD11 is a lysogenic E. faecalis strain, harbouring a wEf11 prophage, which was previously isolated from an infected root canal (Stevens et al, 2009). Following curing, the non-lysogenic variant of this strain was designated E. faecalis TUSoD11 (DwEf11).…”

Section: Methodsmentioning

confidence: 99%

“…wEf11 is a temperate bacteriophage that was induced from a lysogenic root canal isolate of Enterococcus faecalis (Stevens et al, 2009). It is a member of the family Siphoviridae, with a long (130 nm) non-contractile tail and a small (41 nm diameter) spherical/icosohedral head.…”

Section: Introductionmentioning

confidence: 99%

“…The wEf11 DNA has been sequenced and annotated, disclosing a genome of 42 822 bp encoding 65 ORFs (Stevens et al, 2011). Furthermore, our previous studies disclosed that the wEf11 DNA restriction pattern produced with certain restriction enzymes, such as NsiI, produced several fragments in submolar amounts (Stevens et al 2009). This would be expected to occur in the case of a circularly permuted genome due to headful packaging of a concatemeric phage DNA during viral maturation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic modifications to temperate Enterococcus faecalis phage ϕEf11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection

et al. 2013

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic modifications to temperate Enterococcus faecalis phage ϕEf11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection

et al. 2013

Self Cite

View full text Add to dashboard Cite

“…Despite the significance of E. faecalis in root canal infections, very few attempts were made to treat E. faecalis infection using phage therapy (31,32). In the present study, an E. faecalis-infecting phage was isolated from sewage and characterized genetically by sequencing and functionally by evaluating its efficacy against E. faecalis planktonic and biofilm cultures in vitro.…”

mentioning

confidence: 95%

Targeting Enterococcus faecalis Biofilms with Phage Therapy

Khalifa

Brosh

Gelman

et al. 2015

Appl Environ Microbiol

188

158

View full text Add to dashboard Cite

Phage therapy has been proven to be more effective, in some cases, than conventional antibiotics, especially regarding multidrug-resistant biofilm infections. The objective here was to isolate an anti-Enterococcus faecalis bacteriophage and to evaluate its efficacy against planktonic and biofilm cultures. E. faecalis is an important pathogen found in many infections, including endocarditis and persistent infections associated with root canal treatment failure. The difficulty in E. faecalis treatment has been attributed to the lack of anti-infective strategies to eradicate its biofilm and to the frequent emergence of multidrug-resistant strains. To this end, an anti-E. faecalis and E. faecium phage, termed EFDG1, was isolated from sewage effluents. The phage was visualized by electron microscopy. EFDG1 coding sequences and phylogeny were determined by whole genome sequencing (GenBank accession number KP339049), revealing it belongs to the Spounavirinae subfamily of the Myoviridae phages, which includes promising candidates for therapy against Gram-positive pathogens. This analysis also showed that the EFDG1 genome does not contain apparent harmful genes. EFDG1 antibacterial efficacy was evaluated in vitro against planktonic and biofilm cultures, showing effective lytic activity against various E. faecalis and E. faecium isolates, regardless of their antibiotic resistance profile. In addition, EFDG1 efficiently prevented ex vivo E. faecalis root canal infection. These findings suggest that phage therapy using EFDG1 might be efficacious to prevent E. faecalis infection after root canal treatment.

show abstract

“…Studies on saliva have isolated bacteriophages with a) bactericidal effect against specific bacterial pathogens such as Enterococcus faecalis, for example (Stevens et al 2009) and b) transduction property capable of transferring antibiotic resistance genes. The example includes temperate bacteriophage that transfers genes among Actinobacillus actinomycetemcomitans strains (Sandmeier et al 1995, Roy 2015.…”

Section: Mechanism Of Horizontal Gene Transfer In Bacteriamentioning

confidence: 99%

Horizontal Gene Transfer in Plasmid: Are We Close to Eliminating Periodontal Pathogens?

et.al.¹

2017

IJBTR

View full text Add to dashboard Cite

In clinical periodontal practice, patients with periodontitis frequently yield subgingival pathogens, resistant to therapeutic concentrations of commonly used antibiotics. The oral cavity is believed to provide excellent exogenous horizontal DNA transfer in dental plaque bacteria. Plasmids harness the replication, transcription, and translation systems of their host to ensure their continuity. While doing so, they burden their hosts' functionality. The non-specificity and inefficiency of current antibiotics and plasmid-mediated drug resistance is a major emerging challenge in dentistry.

show abstract

Bacteriophages induced from lysogenic root canal isolates of Enterococcus faecalis

Abstract: This is the first report of lysogenic bacteria and their inducible viruses in infected root canals.

Cited by 42 publications

References 44 publications

Genetic modifications to temperate Enterococcus faecalis phage ϕEf11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection

Genetic modifications to temperate Enterococcus faecalis phage ϕEf11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection

Targeting Enterococcus faecalis Biofilms with Phage Therapy

Horizontal Gene Transfer in Plasmid: Are We Close to Eliminating Periodontal Pathogens?

Contact Info

Product

Resources

About