Bacteriological Quality of Marketed Mola Fish, Amblypharyngodon Mola From Dhaka Metropolis

Abstract

The bacteriological quality of mola fish (Amblypharyngodon mola) from three local fish markets as fresh and as frozen from three departmental chain shops of Dhaka metropolis were analyzed. The microbial quality parameters varied with different sources and the quality was found to be poor for local market fish samples. In total 24 samples were considered for bacteriological quality analysis and 10 pathogenic isolates for antibiotic sensitivity test to 12 antibiotics. The total bacterial count ranged from 1.8 ± 0.25×104 to 6.5 ± 0.75 × 106 cfu/g for fresh and 5.5 ± 0.55 × 103 to 7.0 ± 0.80 × 105 cfu/g for frozen mola. The highest total coliform count of mola was 8.0 ± 0.55 × 104 and 6.1 ± 0.40 × 103 cfu/g for local market and departmental chain shop, respectively. All fresh and frozen samples were observed having high quantity of E. coli above 102 cfu/g. Furthermore, Salmonella-Shigella was identified in 67% samples (75% of fresh and 58% of frozen samples) varied from 0.9 ± 0.00 ×102 to 5.3 ± 0.30 × 103 cfu/g whereas Vibrio spp.was confirmed in 79% samples (83% of fresh and 75% of frozen samples) of which 90% samples exceeded 102 cfu/g. Similar pattern was observed in Staphylococcus spp. with 83% of fresh and 58% of frozen samples (63% of total samples) beyond 103 cfu/g. In case of antibiotic sensitivity pattern of the indicator and pathogenic isolates, all of them were resistant to amoxicillin and penicillin. Most of the isolates were sensitive to bacitracin, ciprofloxacin, erythromycin and streptomycin. The findings proved that mola fish under this study was more or less contaminated and local fish market samples were highly privileged with food borne pathogens which confirmed the unhygienic condition of the market as well as the presence of antibiotic resistance bacteria in mola fish may threat to public health safety. DOI: http://dx.doi.org/10.3329/bjz.v40i1.12897 Bangladesh J. Zool. 40(1): 77-88, 2012

Effect of freezing period on the microbial and nutrient quality of pool barb, Puntius sophore from Dhaka city retail market, Bangladesh

“…Nilla et al (2012b) found TCC 2.4 ± 0.30 × 10 3 to 8.0 ± 0.55 × 10 4 cfu/g in A. mola from Dhaka metropolis local markets whereas Begum et al (2010) found highest TC >240 mpn/g in local marketed B. sarana. On the contrary, Zahid et al (2011) did not found TCC in normal P. sarana from local markets.…”

“…The present findings are supported by some previous works of different scientists who worked on punti and small fish. Nilla et al (2012b) found TBC ranged between 1.8 ± 0.25 × 10 4 to 6.5 ± 0.75 × 10 6 cfu/g in mola fish (Amblypharyngodon mola) from local markets of Dhaka metropolis where the whole fish was considered for bacterial quality analysis. Begum et al (2010) found TVBC (total viable bacterial counts) 3.3 × 10 5 to 1.9 × 10 8 cfu/g in sarpunti (Puntius sarana) collected from local markets.…”

“…Nilla et al (2012b) reported sewage contagion in the market as a contamination source of fish. Moreover, the water use for washing or icing may act as contamination sources for fish (Boyd 1990).…”

“…It concerns with animal protein derived from marketed fish, fishery products, meats and meat products that creates a high risk commodity regarding to microbial contaminations, adulterants and natural toxicity (Nilla et al 2012a). Besides, bacteriological *Corresponding author: E-mail: mgmustafabd@yahoo.com 1 Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh. quality is directly related to the spoilage of fish and becomes the cause of outbreak of food poisoning (Nilla et al 2012b). Microbial hazards that are ingested in fish body at the post harvest period cause fish spoilage.…”

“…Low quality frozen processing related with improper and poor hygienic condition (Nilla et al 2012b), consequently contaminate the frozen fish with different types of bacteria spoiling the fish that may be responsible for cholera and other food borne diseases (Mobin et al 2001).…”