Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions

Gök, Üzeyir; Bulut, Yasemin; Keleş, Erol; Yalçin, Süleyman; Doymaz, Mehmet Ziya

doi:10.1016/s0165-5876(01)00510-9

Cited by 86 publications

(71 citation statements)

References 17 publications

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“…These two microorganisms, together with S. pneumoniae, are among the most common infectious agents implicated in both AOM and OME [2,6]. The incidence of bacterial growth in the MEE of our patients was lower than in many previous studies, where the rate varies from 21% to 70% [2][3][4][5][6]. However, such lower rate of positive culture for OME has similarly been reported previously [8,23].…”

Section: Discussionsupporting

confidence: 59%

“…Although OME is one of the most common diseases of childhood, its etiology and pathogenesis are still under debate. OME was considered a sterile condition for a long time; however, recent studies have demonstrated bacterial growth in 21% to 70% of the middle ear effusions (MEE) [2][3][4][5][6]. The most frequently found pathogens are S. pneumoniae, H. influenzae and M. catarrhalis.…”

Section: Introductionmentioning

confidence: 99%

“…During the last decade, several studies have applied more sensitive molecular methods, most often PCR-based assays, for detection of middle ear pathogens in OME. The overall rate of PCR-positive effusions from patients with OME has been significantly higher than by conventional culture, varying from 46% to 100% [3,4,7,8]. The bacteria found by PCR-based assays are usually the same as listed above.…”

Section: Introductionmentioning

confidence: 99%

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Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion

Kasenõmm¹,

Štšepetova²

2012

IJOHNS

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Background: The aim of the study was to analyze the performance of PCR-DGGE based assay and its applicability as a tool for the identification of bacteria in the middle ear of children with otitis media with effusion (OME). Methods:The middle ear effusions from 20 children with OME were analyzed both by bacterial culture and by 16S rDNA-gene-targeted PCR assay, DGGE fingerprinting and sequencing analysis. Results: In bacterial culture assay, only three middle ear effusions (15%) showed bacterial growth. None of the samples were positive for anaerobic culture. The PCR assay with 16S rDNA-gene-targeted universal primers was positive in 10 (50%) cases. The subsequent DGGE fingerprinting and 16S rDNA sequencing analysis revealed that the most commonly encountered bacteria in the middle ear effusions of children with OME are Haemophilus influenzae, Alloiococcus otitidis and Bacteroides spp. Conclusions: The present study demonstrated the applicability of PCR-DGGE based assay and 16S rDNA sequencing for analyzing of bacterial diversity in the middle ear effusion of children OME. The results of our study may contribute to a better understanding of the etiology of OME.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion

Kasenõmm¹,

Štšepetova²

2012

IJOHNS

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“…The sensitivity of PCR was determined with 10-fold serial dilution, from 50 ng to 5 fg, of the purified DNA extracted from the reference strain as described previously (Gok et al 2001). Each dilution was analyzed by PCR.…”

Section: Clo-assaymentioning

confidence: 99%

Association of cagA+ Helicobacter pylori with Adenotonsillar Hypertrophy

Bulut

Agacayak

Karlıdağ

et al. 2006

Tohoku J. Exp. Med.

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“…Although a majority of OM or COPD is mainly associated with a single bacterial pathogen, there is a growing body of evidence that a portion of patients diagnosed with OM or COPD have mixed infections of NTHi and S. pneumoniae [4,5]. Like most other bacterial infections, both OM and COPD are also characterized by inflammation, which is mainly mediated by inflammatory cytokines and chemokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-8 (IL-8) [6,7].…”

mentioning

confidence: 99%

Synergistic activation of NF-κB by nontypeable H. influenzae and S. pneumoniae is mediated by CK2, IKKβ-IκBα, and p38 MAPK

Kweon

Wang

Rixter

et al. 2006

Biochemical and Biophysical Research Communications

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In review of the past studies on NF-κB regulation, most of them have focused on investigating how NF-κB is activated by a single inducer at a time. Given the fact that, in mixed bacterial infections in vivo, multiple inflammation inducers, including both Nontypeable Haemophilus influenzae (NTHi) and S. pneumoniae, are present simultaneously, a key issue that has yet to be addressed is whether NTHi and S. pneumoniae simultaneously activate NF-κB and the subsequent inflammatory response in a synergistic manner. Here, we show that NTHi and S. pneumoniae synergistically induce NF-κB-dependent inflammatory response via activation of multiple signaling pathways in vitro and in vivo. The classical IKKβ-IκBα and p38 MAPK pathways are involved in synergistic activation of NF-κB via two distinct mechanisms, p65 nuclear translocation-dependent and independent mechanisms. Moreover, casein kinase 2 (CK2) is involved in synergistic induction of NF-κB via a mechanism dependent on phosphorylation of p65 at both Ser536 and Ser276 sites. These studies bring new insights into the molecular mechanisms underlying the NF-κB-dependent inflammatory response in polymicrobial infections and may lead to development of novel therapeutic strategies for modulating inflammation in mixed infections for patients with otitis media and chronic obstructive pulmonary diseases. KeywordsSynergistic activation; NF-κB; CK2; Polymicrobial infection Gram-negative bacterium Nontypeable Haemophilus influenzae (NTHi) and Gram-positive bacterium Streptococcus pneumoniae (S. pneumoniae) are important human pathogens [1,2].

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Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions

Cited by 86 publications

References 17 publications

Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion

Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion

Association of cagA+ Helicobacter pylori with Adenotonsillar Hypertrophy

Synergistic activation of NF-κB by nontypeable H. influenzae and S. pneumoniae is mediated by CK2, IKKβ-IκBα, and p38 MAPK

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