Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion

Kasenõmm, Priit; Štšepetova, Jelena

doi:10.4236/ijohns.2012.13015

Cited by 3 publications

(3 citation statements)

References 26 publications

(48 reference statements)

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“…polymerase chain reaction), recent advances in genomic technologies now allow for the analysis of entire polymicrobial communities (‘microbiomes’). Bacterial community fingerprinting has been used to profile polymicrobial samples, providing insights into the bacterial richness of otitis media specimens 62 . Marsh 63 used terminal restriction fragment length polymorphism analysis to investigate middle-ear discharge in Indigenous children with acute otitis media with perforation for up to six weeks, and found significant differences between the bacterial communities of nasopharyngeal and middle-ear discharge specimens.…”

Section: Resultsmentioning

confidence: 99%

Microbiology of otitis media in Indigenous Australian children: review

et al. 2017

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Most studies have used culture-based methods and specimens from middle-ear discharge or the nasopharynx. Findings from these studies are consistent with international literature, but reliance on culture may incorrectly characterise the microbiology of this condition. Advances in genomic technologies are now providing microbiologists with the ability to analyse the entire mixed bacterial communities ('microbiomes') of samples obtained from Indigenous children with otitis media.

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Section: Resultsmentioning

confidence: 99%

Microbiology of otitis media in Indigenous Australian children: review

et al. 2017

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“…Apart from conventional and multiplex PCR in which the pathogens are targeted with specific primer, some advanced techniques like PCR-Denaturing gradient gel electrophoresis (PCR-DGGE), 16S rRNA based Next generation sequencing (NGS) and MALDI-TOF MS are able to detect possible bacteria species present in a clinical sample. (23,75,76). Thus, molecular techniques have further exposed and enhanced the identification of A. actinomycetemcomitans, P. gingivalis, T. denticola, F. nucleatum, H influenzae, A. otitis and Bacteroids spp.…”

Section: Molecular Techniquesmentioning

confidence: 99%

“…Thus, molecular techniques have further exposed and enhanced the identification of A. actinomycetemcomitans, P. gingivalis, T. denticola, F. nucleatum, H influenzae, A. otitis and Bacteroids spp. in middle ear effusion (23,44,67,75).…”

Section: Molecular Techniquesmentioning

confidence: 99%

Application of Anaerobic Techniques in Laboratory Diagnosis of Otitis Media in Nigeria: A Review

Nwaokorie,

Nwokoye,

Chukwu

2017

JBMS

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Otitis media is a global disease with severe health, social and economic burdens. In Nigeria, there are limited data on the profile of anaerobic species associated with the categories of otitis media. The focus of most laboratory diagnosis for otitis media has been on aerobic organisms, the very few that considered anaerobes often use unsuitable standard anaerobic techniques that often lead to misdiagnosis. In other to prevent antibiotic resistance, treatment failure, recurrent infection and associated complication in cases of patients with otitis media, proper laboratory techniques should be utilized to ascertain the pathogens hence, anaerobes should not be overlooked. This review therefore underscores the standard methods of anaerobic techniques being utilized in the laboratory diagnosis of otitis media in Nigeria.

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Analysis of microbial diversity in apple vinegar fermentation process through 16s rDNA sequencing

Song

Zhang

Kang

et al. 2019

Food Science & Nutrition

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Based on SPME‐GC‐MS analysis, it could be found that the production of acetic acid, phenethyl acetate, and isoamyl acetate gradually increased in the apple vinegar fermentation broth with the fermentation time. Consequently, in order to systematically explore the dynamic changes of microbial diversity and metabolites in the process of apple vinegar fermentation, 16S rDNA were sequenced and analyzed in this work. The present results showed that bacterial diversity was rich and exhibited a certain variation during the dynamic fermentation process of apple vinegar. Furthermore, Lactococcus and Oenococcus were the predominant bacteria in the pre‐fermentation (alcoholic fermentation) of apple vinegar, while the dominant bacteria in the middle and late fermentation stages (acetic acid fermentation) were Lactococcus and Acetobacter . In addition, during the whole fermentation process of apple vinegar, Lactococcus was the most dominant bacteria, Oenococcus was the unique species in the stage of alcohol fermentation, and Acetobacter increased rapidly in the stage of acetic acid fermentation. In conclusion, our finding provided a theoretical basis for the processing technology of apple vinegar fermentation, and a theory evidence for the safety and health assessment of apple vinegar.

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Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion

Cited by 3 publications

References 26 publications

Microbiology of otitis media in Indigenous Australian children: review

Microbiology of otitis media in Indigenous Australian children: review

Application of Anaerobic Techniques in Laboratory Diagnosis of Otitis Media in Nigeria: A Review

Analysis of microbial diversity in apple vinegar fermentation process through 16s rDNA sequencing

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