Bacteriocin-producing lactic acid bacteria as a probiotic potential from Thai indigenous chickens

Musikasang, H.; Sohsomboon, N.; Tani, Akio; Maneerat, Suppasil

doi:10.17221/5568-cjas

Cited by 18 publications

(13 citation statements)

References 32 publications

(42 reference statements)

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“…It is well known that resistance of the indigenous laying or meat chickens (especially those produced in village societies) to different biotic and abiotic stresses, is related to presence of LAB strains with high probiotic potentials in the digestive systems of such chickens (Damayanti et al, 2012;Musikasang et al, 2012;Yamazakia et al, 2012). The presence of some lactobacilli bacteria in the chicken GIT has been described to be of great importance for regulating the composition of the intestinal microflora, developing immunity of the intestine, and promoting the health of chickens (Yamazakia et al, 2012 ;Muir et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Lactic acid bacteria (LAB), as common microorganisms in foods and the intestine of humans and most animals, are widely used as probiotics in humans and animals to restore the ecological balance of different mucosa (Chen et al, 2012;Musikasang et al, 2012). Some studies showed high effect of LAB on the economic yield properties (lay intensi-…”

Section: Introductionmentioning

confidence: 99%

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Characterization of some potentially probiotic Lactobacillus strains isolated from Iranian native chickens

Aazami

Jouzani

Khodaei

et al. 2014

J. Gen. Appl. Microbiol.

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The objective of the present study was to isolate, identify and characterize new LAB strains with high probiotic potentials from Iranian (Isfahan) indigenous chickens. From 90 isolated LABs, 11 isolates had high growth rate under different stress conditions, including acid (pH 2.5), bile (0.5% oxgall), salt (6 15%) and temperatures 15 and 45 C, and their aggregation time was less than 120 min. Based on the molecular identification using 16S rDNA sequencing and phylogenetic analysis, the isolates belonged to two Lactobacillus salivarius and L. reuteri species. The isolates showed different tolerance to 16 clinically and veterinary relevant antibiotics, and most of them were resistant to or semitolerant of 7 15 different studied antibiotics. The Es11, Es12, Es3 and Es13 strains with resistance to or semi-tolerance of 15, 14 and 13 different antibiotics, respectively, were the most tolerant strains. The selected isolates showed a wide range of antimicrobial activity against 7 different pathogenic strains. All the isolates exhibited antagonistic activity against E. coli, Enterococcus hirae, Salmonella enterica and Staphylococcus aureus. The isolates Es6 and Es11 with high antagonistic activity and resistance against 6 of the studied pathogens were the most powerful antagonistic isolates. The values and types of adhesion to the Caco-2 cell cultures were significantly different (0 40 bacteria/Caco-2 cell), and the maximum adhesion was observed for the isolates Es6 and Es13 with 35 and 40 bacteria adhesion/cell, respectively. Finally, based on all the experiments, 7 strains, including Es1, Es6, Es7, Es11, Es12 and Es13, were selected for the further in vivo assays and possible use in the poultry industry.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of some potentially probiotic Lactobacillus strains isolated from Iranian native chickens

Aazami

Jouzani

Khodaei

et al. 2014

J. Gen. Appl. Microbiol.

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“…However, the isolates could produce protease and phytase (Table 6). Musikasang et al (2012) found that LAB isolates showed the proteinase activity, but neither starch nor lipid digestions were detected. Selected LAB isolates can produce phytase that hydrolyzes phytic acid to myo-inositol and phosphoric acid.…”

Section: Assessment Of Probiotic Characteristicsmentioning

confidence: 90%

Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics

Jannah¹,

Dinoto²,

Wiryawan³

et al. 2014

Med Pet

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The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic ABSTRAK Tujuan penelitian ini adalah untuk menyeleksi dan mengarakterisasi bakteri asam laktat (BAL) yang diisolasi dari saluran pencernaan ayam Cemani, salah satu ayam asli Indonesia dan untuk mengetahui potensi penggunaannya sebagai probiotik. BAL diisolasi dari saluran pencernaan dengan menggunakan medium MRSA dan GYPA dan diinkubasi secara anaerobik. BAL terpilih kemudian ditentukan karakter probiotiknya melalui serangkaian percobaan. Identifikasi isolat BAL terpilih berdasarkan pada sekuen 16S rDNA, karakter morfologi dan biokimianya. Sembilan puluh lima isolat diisolasi dan dikarakterisasi sebagai bakteri asam laktat (Gram positif, katalase negatif, tidak membentuk endospora dan menghasilkan asam). Dua puluh empat isolat BAL memperlihatkan aktivitas antimikrob terhadap E. coli JCM 1649 dan S. enteritidis B2586, dan tiga isolat terpilih, yaitu CCM011, CSP004, dan CVM002 menunjukkan aktivitas penghambatan yang tertinggi. Isolat-isolat tersebut mempunyai karakter pelekatan terhadap permukaan sel yang tinggi, mempunyai kemampuan koagregasi antar BAL, daya tahan yang tinggi pada pH rendah, menunjukkan aktivitas enzim fitase dan protease yang tinggi (tetapi tidak mempunyai aktivitas amilase dan lipase), koagregasi yang lemah terhadap bakteri patogen dan tidak resisten terhadap antibiotik uji. Isolat-isolat tersebut diidentifikasi berdasarkan analisis sekuen gen 16S rRNA sebagai Lactobacillus salivarius, tetapi tiap isolat mempunyai profil fermentasi terhadap gula yang berbeda. Ketiga isolat BAL tersebut berpotensi seba...

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“…Musikasang et al [23] checked antibiotic sensitivity of their isolated LABs against various antibiotics. To penicillin G, all LAB strains showed high susceptibility to erythromycin (MIC < 0.25 μg/ml) and tetracycline.…”

Section: Discussionmentioning

confidence: 99%

Potential use of bacteriocin producing lactic acid bacterial strain isolated from milk products and its application as the fish feed

Khumbongmayum¹,

Veenagayathri²

2016

Int. J. Sci. World

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Bacteriocins are gene-encoded inhibitory proteins and those produced by Gram-positive Lactic acid bacteria. Some bacteriocins even display antagonistic activity towards Gram-positive food borne pathogens and spoilage organisms. This present study involves isolation of Bacteriocin-producing lactic acid bacteria from a variety of milk and milk products. The physico-chemical properties of the isolated bacteriocin producing bacterial strains were screened. The isolated bacteriocin bacterial strains were biochemically characterized and identified. Further, the isolated effective bacterial strain was used as a fish feed and its effect on their growth was evaluated. The evaluated data continue to demonstrate that the bacteriocin producing bacterial strains will have greater potential in the food products industry.

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Bacteriocin-producing lactic acid bacteria as a probiotic potential from Thai indigenous chickens

Cited by 18 publications

References 32 publications

Characterization of some potentially probiotic Lactobacillus strains isolated from Iranian native chickens

Characterization of some potentially probiotic Lactobacillus strains isolated from Iranian native chickens

Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics

Potential use of bacteriocin producing lactic acid bacterial strain isolated from milk products and its application as the fish feed

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