Characterization of some potentially probiotic Lactobacillus strains isolated from Iranian native chickens

Abstract

The objective of the present study was to isolate, identify and characterize new LAB strains with high probiotic potentials from Iranian (Isfahan) indigenous chickens. From 90 isolated LABs, 11 isolates had high growth rate under different stress conditions, including acid (pH 2.5), bile (0.5% oxgall), salt (6 15%) and temperatures 15 and 45 C, and their aggregation time was less than 120 min. Based on the molecular identification using 16S rDNA sequencing and phylogenetic analysis, the isolates belonged to two Lactobacillus salivarius and L. reuteri species. The isolates showed different tolerance to 16 clinically and veterinary relevant antibiotics, and most of them were resistant to or semitolerant of 7 15 different studied antibiotics. The Es11, Es12, Es3 and Es13 strains with resistance to or semi-tolerance of 15, 14 and 13 different antibiotics, respectively, were the most tolerant strains. The selected isolates showed a wide range of antimicrobial activity against 7 different pathogenic strains. All the isolates exhibited antagonistic activity against E. coli, Enterococcus hirae, Salmonella enterica and Staphylococcus aureus. The isolates Es6 and Es11 with high antagonistic activity and resistance against 6 of the studied pathogens were the most powerful antagonistic isolates. The values and types of adhesion to the Caco-2 cell cultures were significantly different (0 40 bacteria/Caco-2 cell), and the maximum adhesion was observed for the isolates Es6 and Es13 with 35 and 40 bacteria adhesion/cell, respectively. Finally, based on all the experiments, 7 strains, including Es1, Es6, Es7, Es11, Es12 and Es13, were selected for the further in vivo assays and possible use in the poultry industry.

Evaluation of Probiotic Potential of Some Native Lactobacillus Strains on the Growth Performance and Serum Biochemical Parameters of Japanese Quails (Coturnix Coturnix Japonica) during Rearing Period

“…Four native Lactobacilli strains with probiotic potential, including OR7 (L. crispatus), Es7 (L. salivarius), OR 10 (L. crispatus), and M4 (L. oris), were evaluated in this study (Aazami et al, 2014). The strains were separately cultured in skim milk medium at 37°C and pH 5.6 for 24 h in a 10-L batch bioreactor (Bioflo 2000, New Brunswick, USA).…”

“…The strains presented fast auto-aggregation rate (10 to 120 min) and high ability for adhesion to the Caco-2 epithelial cell line (up to 40 bacterial cells). High antimicrobial activities against different pathogens (Pseudomonas aeroginosa, E. coli, Streptococcus mutans, Clostridium difficile, Enterococcus hirea, Salmonella enterica, Staphylococcus aureus) were detected in vitro (Aazami et al, 2014). The aim of the present study was to evaluate the probiotic effects of these selected native Lactobacillus strains on the growth performance and serum biochemical parameters of Japanese quails.…”

“…There is a huge body of evidence that support the significant positive impacts of probiotics and bioactive compounds on poultry performance and health. Probiotic bacteria improve the economic indexes and resistance to pathogens of laying or meat-type chickens (Hippenstiel et al, 2011;Aazami et al, 2014;Cean et al, 2015;Mountzouris et al, 2007). Lactobacilli and Enterococci have been widely used as probiotics in the poultry industry (Kabir et al, 2004;Awad et al, 2009;Aazami et al, 2014;Mountzouris et al, 2007).…”

“…Probiotic bacteria improve the economic indexes and resistance to pathogens of laying or meat-type chickens (Hippenstiel et al, 2011;Aazami et al, 2014;Cean et al, 2015;Mountzouris et al, 2007). Lactobacilli and Enterococci have been widely used as probiotics in the poultry industry (Kabir et al, 2004;Awad et al, 2009;Aazami et al, 2014;Mountzouris et al, 2007). The main postulated health benefits associated with probiotics include improving the gut microflora balance, stimulating the immune reaction, producing different antimicrobial substances, Siadati SA, Ebrahimnezhad Y, Salehi Jouzani G, Shayegh J Evaluation of Probiotic Potential of Some Native Lactobacillus Strains on the Growth Performance and Serum Biochemical Parameters of Japanese Quails (Coturnix Coturnix Japonica) during Rearing Period modulating the immune response, producing digestive enzymes, and reducing cholesterol levels (RamirezChavarin et al, 2013;Smug et al, 2014).…”

“…Our group previously isolated and characterized some potentially probiotic bacteria from Iranian native chickens (Aazami et al, 2014). The selected Lactobacillus strains showed high resistance to acidic gastric conditions (pH 2.5) and to bile salts (0.5% Oxgall), as well as to salinity (up to 15% NaCl) and low and high temperatures (between 4.5 and 45°C).…”