Lactobacillus spp. impair the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells

Castellano, Patricia; Ibarreche, Mariana Pérez; Borges, Liliana Longo; Arias, Florentino; Ross, Gloria Romina; Martinis, Elaine Cristina Pereira De

doi:10.1007/s10529-018-2572-x

Cited by 12 publications

(5 citation statements)

References 21 publications

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“…The adhesion and invasion abilities of L. monocytogenes to Caco-2 cells are reduced when L. monocytogenes is co-cultured with Lactobacillus spp. [ 68 ], Lactobacillus rhamnoides [ 69 ], and L. plantarum [ 55 ]. Moroni et al observed that the adhesion and invasion ability of L. monocytogenes to colonocytes was reduced by Lactobacillus [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum subsp. plantarum and Fructooligosaccharides Combination Inhibits the Growth, Adhesion, Invasion, and Virulence of Listeria monocytogenes

Dong

Gao

et al. 2022

Foods

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Listeria monocytogenes is a foodborne pathogen responsible for many food outbreaks worldwide. This study aimed to investigate the single and combined effect of fructooligosaccharides (FOS) and Lactiplantibacillus plantarum subsp. plantarum CICC 6257 (L. plantarum) on the growth, adhesion, invasion, and virulence of gene expressions of Listeria monocytogenes 19112 serotype 4b (L. monocytogenes). Results showed that L. plantarum combined with 2% and 4% (w/v) FOS significantly (p < 0.05) inhibited the growth of L. monocytogenes (3–3.5 log10 CFU/mL reduction) at the incubation temperature of 10 °C and 25 °C. Under the same combination condition, the invasion rates of L. monocytogenes to Caco-2 and BeWo cells were reduced more than 90% compared to the result of the untreated group. After L. plantarum was combined with the 2% and 4% (w/v) FOS treatment, the gene expression of actin-based motility, sigma factor, internalin A, internalin B, positive regulatory factor A, and listeriolysin O significantly (p < 0.05) were reduced over 91%, 77%, 92%, 89%, 79%, and 79% compared to the result of the untreated group, respectively. The inhibition level of the L. plantarum and FOS combination against L. monocytogenes was higher than that of FOS or L. plantarum alone. Overall, these results indicated that the L. plantarum and FOS combination might be an effective formula against L. monocytogenes.

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Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum subsp. plantarum and Fructooligosaccharides Combination Inhibits the Growth, Adhesion, Invasion, and Virulence of Listeria monocytogenes

Dong

Gao

et al. 2022

Foods

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“…Survival of passage through the challenging conditions in the digestive tract and adhesion to human cells are important for strains to show their health effects in the human gut. The resistance to such conditions is crucial, as exposure to bile salts causes dissociation of the lipid bilayer of cell membranes and disruption of the integrity of cells [45][46][47][48][49][50][51][52][53][54]. Consumption of approximately 1.0x10 6 to 1.0x10 10 viable cells per day is generally accepted as the minimum limit required in order to gain satisfactory probiotic functions in the intestine [15,[55][56][57].…”

Section: Discussionmentioning

confidence: 99%

“…Studies also concluded that coaggregation, autoaggregation and adhesion abilities are strain specific. This specificity is determined by surface proteins, glycoproteins, teichoic acids and lipoteichoic acids, as well as S-layer proteins [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60]. The strains used in our study showed high rates of adhesion to intestinal epithelial cells (Table 4).…”

Section: Discussionmentioning

confidence: 99%

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Determination of competition and adhesion abilities of lactic acid bacteria against gut pathogens in a whole-tissue model

Alp

Kuleaşan

2020

Bioscience of Microbiota, Food and Health

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In an intestinal system with a balanced microbial diversity, lactic acid bacteria (LAB) are the key element which prevents the colonization and invasion of gut pathogens. Adhesion ability is important for the colonization and competition abilities of LAB. The aim of this study was to determine the adhesion and competition abilities of LAB by using a whole-tissue model. Indigenous strains were isolated from spontaneously fermented foods like cheese and pickles. The aggregation and competition abilities of the isolates were determined, as well as their resistance to gastrointestinal conditions. Four Lactobacillus strains and one Weissella strain were found to be highly competitive against three major gut pathogens, namely Clostridium difficile , Listeria monocytogenes and Salmonella Enteritidis. Tested strains decreased the number of pathogens to below their disease-causing levels. According to the results, the numbers of C. difficile and L. monocytogenes bacteria decreased by an average of 3 log, and their adhesion rates decreased by approximately 50%. However, the number of S. Enteritidis bacteria was decreased by only 1 log compared with its initial number. We thought that the weak effect on Salmonella was due to its possession of many virulence factors. The results showed that natural isolates from sources other than human specimens like the Weissella strain in this study were quite competent when compared with the human isolates in terms of their adhesion to intestines and resistance to gastrointestinal tract conditions. It was also revealed that a whole-tissue model with all-natural layers can be successfully used in adhesion and competition tests.

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“…The effective growthpromoting and protective effect of the oligosaccharide FOS in his study could be attributed to the preference of L. fermentum cells entrapped to FOS encapsulated. At a deeper level, 33 revealed that microencapsulation with oligosaccharides allowed viable L. fermentum cells to guarantee a high level for use as a probiotic during intestinal simulation, based on the usual effective dosage of 10 7 -10 9 CFU/ml.…”

Section: Survival Of Microencapsulated Cells Of 3 Probiotic Strains I...mentioning

confidence: 99%

Co-effect of microencapsulation and prebiotics on the survivability of some lactic acid bacteria in simulating gastrointestinal tract and storage conditions

Gharieb,

Bresha,

Omar

2024

Journal of Modern Research

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Probiotics lose their viability during formulation, processing, and storage. The current work investigates the co-effect of three different combinations of encapsulation and prebiotics on the Survival of L. rhamnosus, L. acidophilus, and B. adolescentis under different conditions. In simulating gastric juice solution, the free cells survivability ranged between 36.5% to 40.5% for B. adolescentis and L. rhamnosus, after 2 hr, respectively. However, the encapsulated bacteria survival, ranged between 54.5% to 78.5% for B. adolescentis and L. rhamnosus, respectively. The encapsulated bacteria exhibited the highest survival rates, between 78.5%, and 76.5% for L. rhamnosus, and L. acidophilus, respectively, and 68.7% for B. adolescentis against the enzymatic gastric juice. In the simulating intestinal juice solution, cells encapsulated with resistant starch (ARs) and oligosaccharides (ARsG or ARsF) significantly enhanced survival over bacteria encapsulated with alginate alone and free cells, where the survivability was 104.4% for L. rhamnosus, 103.4% for L. acidophilus and 103.6% for B. adolescentis . A highly significant difference in survival rates was found between encapsulated and nonencapsulated bacteria when stored at 4 o C and 25 o C for 30 days. Survivability between 31.5% to 77.1% was apparent for L. acidophilus and L. rhamnosus, respectively, after 30 days at 4 o C. In contrast, free bacterial cells recorded a 29.1% to 31.5% survivability. After 30 days, the survivability of microencapsulated bacteria at 25 o C ranged between 15.6% and 63.6%, while the survival rate of free bacteria declined between 10.9% and 13.5%. Overall, microencapsulation of the tested strains enhanced bacteria tolerance, survival, and storage periods, especially at 4 o C.

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Lactobacillus spp. impair the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells

Cited by 12 publications

References 21 publications

Lactiplantibacillus plantarum subsp. plantarum and Fructooligosaccharides Combination Inhibits the Growth, Adhesion, Invasion, and Virulence of Listeria monocytogenes

Lactiplantibacillus plantarum subsp. plantarum and Fructooligosaccharides Combination Inhibits the Growth, Adhesion, Invasion, and Virulence of Listeria monocytogenes

Determination of competition and adhesion abilities of lactic acid bacteria against gut pathogens in a whole-tissue model

Co-effect of microencapsulation and prebiotics on the survivability of some lactic acid bacteria in simulating gastrointestinal tract and storage conditions

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