Bactericidal and Antioxidant Activity of Essential Oils from Myristica fragrans Houtt and Salvia microphylla H.B.K

Abstract

This study chemically characterizes and evaluates the bactericidal and antioxidant activities of essential oils from Myristica fragrans and Salvia microphylla. The essential oils were obtained by steam distillation and were subsequently subjected to analysis by GC-MS and GC. The agar diffusion test was employed to evaluate the bactericidal activity, while the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and b-carotene/linoleic acid tests were used to determine the antioxidant activity. Terpin-4-ol (14.95%), sabinene (13.07%) and c-terpinene (11.22%) were found to be the major constituents in the essential oil of M. fragrans by gas chromatography, whereas (E)-caryophyllene (15.35%), a-eudesmol (14.06%), b-eudesmol (8.74%) and c-eudesmol (7.64%) were encountered in the essential oil of S. microphylla. Both essential oils showed bactericidal activity, with the essential oil of S. microphylla being more efficient. The antioxidant activity of the essential oils from M. fragrans and S. microphylla were demonstrated by the b-carotene/linoleic acid test, with IC 50 976 and IC 50 770 lg/mL, respectively.

Characterization of the Biological Potential of the Essential Oils from Five Species of Medicinal Plants

“…After incubation for 60 minutes in the absence of light, readings were performed in a spectrophotometer at a wavelength of 515 nm. Comparison tests were conducted with synthetic BHT (butylated hydroxytoluene) at the same concentration [10].…”

essential oil (EO) composition and antioxidant activity of two Salvia leriifolia Benth. (Lamiaceae) populations from Iran

“…N: Neyshabur; B: Bajestan On the contrary, the EO of S. leriifolia was almost inactive in DPPH test of the current study and an inhibition percentage of less than 38% was recorded for the oil concentrations up to 16 μl/ml. EO's generally have low solubility in DPPH method; therefore, the DPPH test could not be reliable for the measurement of the antioxidant activities of such materials (Lima et al, 2012). In this survey, however, the EO's from S. leriifolia in Bajestan and Neyshabur had IC50 values of 5.7 and 2.7 mg/ml in BCB test and 18.9 and 15.2 mg-/ml in TBARS assay.…”

“…Moreover, the anti-oxidant activities of EOs belonging to different Salvia species such as S. officinalis (IC 50 values 22 mg/ml), S. aramienesis (IC 50 values 12.26 mg/ml), S. aucheri (IC 50 values 0.018 mg/ml) and S. pilifera (IC 50 values 0.024 mg/ml) have been demonstrated by DPPH assay and for S. microphylla (IC 50 values 0.77 mg/ml) have been measured by BCB test (Lima et al, 2012;Bouajaj et al, 2013;Kose et al, 2013). The EO of S. eremophila was almost inactive in DPPH and acting weakly in BCB test (Ebrahimabadi et al, 2010).…”

Antioxidant Activity of N-Hexane Extract of Nutmeg Plants From South Aceh Province

“…[5] mempunyai aktivitas antibakteri sedangkan bunga segar pala mempunyai aktivitas antioksidan yang kuat [6]. Senyawa seperti -karoten dan terpenoid dalam minyak atsiri pala juga berpotensi sebagai antioksidan dan antibakteri [7].…”