Antioxidant Activity of N-Hexane Extract of Nutmeg Plants From South Aceh Province

Abstract

Abstract. It has tested the antioxidant activity of n-hexane extract respective roots, bark, fruit and seed crops of nutmeg against DPPH. Each extracted by maceration method with n-hexane. The extraction of each plant nutmeg with n-hexane extract obtained yield of n-hexane respectively 8,35%; 81,5%; 11,89% and 55,63%. The result of the antioxidant activity of n-hexane extract of each plant nutmeg against DPPH with a concentration of 25 ppm, 50 ppm and 100 ppm is obtained IC 50 , respectively, are 0,216 ppm, 63,755 ppm, 43,998 ppm and 11,599 ppm and positive control vitamin C ( IC 50 = 3,657 ppm). Vitamin C is a standard compound is more often used than butyl for very high antioxidant activity. The antioxidant activity increases from high to low at the roots, seeds, fruits and bark of plants nutmeg in a row. Based on data GC-MS chemical components of each successive extracts of roots, bark, fruit and seeds are 41, 41, 44, 43 components of secondary metabolites. The main components of each part of the plant based on data from GC-MS are a 5-Octadecanoic acid, linalool, eugenol, myristicin, methoxyeugenol. The compounds are thought to play an active role in inhibiting free radicals from DPPH. n-Hexane extract nutmeg plant potential as an antioxidant.

ISOLATION OF ESSENSIAL OIL OF NUTMEG (Myristica fragrans Houtt) and ANTIOXIDANT ACTIVITY TEST WITH DPPH

“…As a comparative do the test of antioxidant activity of vitamin C. The main vitamin C solution is made by dissolving 3 mg of vitamin C dissolved with methanol until the volume is exactly 5 mL, further diluted to 3, 6, 9, 12 and 15 ppm (Table 2). Further homogenized with vortex mixer and incubated for 30 min at 37°C, measured uptake at a wavelength of 517 nm [15]. The blank solution test, 1 mL of 0.4 mM DPPH solution and volume of 5 mL with methanol in a test tube (which is covered with aluminum foil), then homogenized with a vortex mixer and incubated for 30 min at 37°C, further measured uptake at wavelength 517 nm using UV-Vis instrument.…”

Isolation Of Antioxidant Compounds Of N-Hexane Extract Of Nutmeg (Myristica Fragrans Houtt) Leaves

“…Prosedur uji antioksidan ekstrak, uji antioksidan vitamin C dan perhitungan IC 50 merujuk pada Ginting, [5]. Untuk mengetahui kekuatan aktivitas antioksidan dari ekstrak nheksana daun pala maka perlu dilakukan uji aktifitas antioksidan dari vitamin C sebagai uji pembanding, karena vitamin C merupakan senyawa dengan aktivitas antioksidan yang sangat tinggi.…”

“…Berdasarkan Tabel 1 menunjukkan bahwa hasil uji fitokimia ekstrak n-heksana daun pala mengandung senyawa metabolit sekunder golongan terpenoid, dan flavonoid sama seperti pada daun segar. Hal ini sesuai dengan penelitian Ginting [5] yang melaporkan bahwa hasil uji fitokimia ekstrak n-heksana bagian akar, kulit batang, buah dan biji tanaman pala juga mengandung senyawa metabolit sekunder golongan terpenoid dan flavonoid.…”

“…Adanya gugus OH dari masing-masing senyawa tersebut di atas diduga yang menyumbang aktivitas antioksidan dan bekerja secara sinergis. Mekanisme kerja penghambatan radikal bebas DPPH yaitu radikal H dari senyawa metabolit sekunder lebih mudah putus karena terikat dengan atom oksigen yang bersifat lebih elektronegatif sehingga H dapat mengikat radikal bebas dari DPPH [5].…”

A Study of Antioxidant Activities in Guava Fruit: Solvents Effect

“…Antioxidant compounds in Kintamani orange and Jember orange can be extracted by ethyl acetate [13]. Hexane can extract antioxidant compounds of nutmeg [14].…”