Bactericidal activity of tracheal antimicrobial peptide against respiratory pathogens of cattle

Taha-Abdelaziz, Khaled; Perez-Casal, José; Schott, Courtney R.; Hsiao, Jason; Attah-Poku, Samuel K.; Slavić, Ðurđa; Caswell, Jeff

doi:10.1016/j.vetimm.2012.12.016

Cited by 34 publications

(38 citation statements)

References 21 publications

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“…CMIR was also associated with the β-defensins which have a variety of roles in protection from pathogens and regulation of immune responses and reproduction [53]. More specifically, CMIR was associated with tracheal associated protein (TAP), a β-defensin which has been found to be important in killing bacteria that can cause pneumonia in cattle [54]. Candidate genes associated with the classical pathway of the complement system were also found to be associated with both AMIR and CMIR.…”

Section: Discussionmentioning

confidence: 99%

A genome-wide association study of immune response traits in Canadian Holstein cattle

et al. 2014

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BackgroundBreeding for enhanced immune response (IR) has been suggested as a tool to improve inherent animal health. Dairy cows with superior antibody-mediated (AMIR) and cell-mediated immune responses (CMIR) have been demonstrated to have a lower occurrence of many diseases including mastitis. Adaptive immune response traits are heritable, and it is, therefore, possible to breed for improved IR, decreasing the occurrence of disease. The objective of this study was to perform genome-wide association studies to determine differences in genetic profiles among Holstein cows classified as High or Low for AMIR and CMIR. From a total of 680 cows with immune response phenotypes, 163 cows for AMIR (81 High and 82 Low) and 140 for CMIR (75 High and 65 Low) were selectively genotyped using the Illumina Bovine SNP50 BeadChip. Results were validated using an unrelated population of 164 Holstein bulls IR phenotyped for AMIR and 146 for CMIR.ResultsA generalized quasi likelihood score method was used to determine single nucleotide polymorphisms (SNP) and chromosomal regions associated with immune response. After applying a 5% chromosomal false discovery rate, 186 SNPs were significantly associated with AMIR. The majority (93%) of significant markers were on chromosome 23, with a similar peak found in the bull population. For CMIR, 21 SNP markers remained significant. Candidate genes within 250,000 base pairs of significant SNPs were identified to determine biological pathways associated with AMIR and CMIR. Various pathways were identified, including the antigen processing and presentation pathway, important in host defense. Candidate genes included those within the bovine Major Histocompatability Complex such as BoLA-DQ, BoLA-DR and the non-classical BoLA-NC1 for AMIR and BoLA-DQ for CMIR, the complement system including C2 and C4 for AMIR and C1q for CMIR, and cytokines including IL-17A, IL17F for AMIR and IL-17RA for CMIR and tumor necrosis factor for both AMIR and CMIR. Additional genes associated with CMIR included galectins 1, 2 and 3, BCL2 and β-defensin.ConclusionsThe significant genetic variation associated with AMIR and CMIR in this study may imply feasibility to include immune response in genomic breeding indices as an approach to improve inherent animal health.

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Section: Discussionmentioning

confidence: 99%

A genome-wide association study of immune response traits in Canadian Holstein cattle

et al. 2014

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“…Tracheal epithelium is a dynamic sensor of threats entering into the lung and responds to pathogens by providing a physical barrier, a mucociliary escalator, and by secreting various antimicrobial peptides, immununomodulatory proteins, antioxidants and chemokines that attract other cells of both the innate and adaptive immune responses. Tracheal antimicrobial peptide is secreted from the respiratory epithelium in an inducible manner and has bactericidal activity against the major pathogens that cause pneumonia in cattle [1]. Other functions include antiviral effects, CCR6-dependent chemotaxis of immature dendritic cells and T lymphocytes, and epithelial cell proliferation and wound repair [38].…”

Section: Discussionmentioning

confidence: 99%

“…Tracheal antimicrobial peptide is an inducible β-defensin that is highly expressed in the bovine respiratory tract and has demonstrated antimicrobial effects on pathogens that cause bovine respiratory disease, including Mannheimia haemolytica, Histophilus somni and Pasteurella multocida [1][2][3]. TAP expression is induced by several pro-inflammatory cytokines as well as bacterial components such as lipopolysaccharide (LPS) [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Comparison of innate immune agonists for induction of tracheal antimicrobial peptide gene expression in tracheal epithelial cells of cattle

Berghuis

Abd-Elaziz

Bierworth

et al. 2014

Vet Res

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Bovine respiratory disease is a complex of bacterial and viral infections of economic and welfare importance to the beef industry. Although tracheal antimicrobial peptide (TAP) has microbicidal activity against bacterial pathogens causing bovine respiratory disease, risk factors for bovine respiratory disease including BVDV and stress (glucocorticoids) have been shown to inhibit the induced expression of this gene. Lipopolysaccharide is known to stimulate TAP gene expression, but the maximum effect is only observed after 16 h of stimulation. The present study investigated other agonists of TAP gene expression in primary cultures of bovine tracheal epithelial cells.

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“…Stimulation with LPS or Pam3CSK induces secretion of LAP, TAP, and lactoferrin, preparing the tissues to ward off invading bacterial pathogens. 11,12 TAP has bactericidal activity against M haemolytica, H somni, and Pasteurella multocida, 13 whereas BMAP-28 can kill P multocida in vitro. 14 Viral infections can interfere with the production of antimicrobial peptides by epithelial cells.…”

Section: Airway and Lung Epithelia And Resistance To Bovine Respiratomentioning

confidence: 99%

“…84 In vitro, TAP has potent bactericidal activity against M haemolytica, P multocida, and H somni, although Mycoplasma bovis is resistant to TAP treatment. 13 In a recent study, TAP was administered therapeutically, via aerosol or intranasal administration, to neonatal calves that had been challenged with M haemolytica. 85 Unfortunately, TAP treatment had little effect on M haemolytica disease.…”

Section: Antimicrobial Peptides As Alternatives To Antibiotics For Bomentioning

confidence: 99%

The Immunology of Bovine Respiratory Disease

McGill

Sacco

2020

Veterinary Clinics of North America: Food Animal Practice

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Bactericidal activity of tracheal antimicrobial peptide against respiratory pathogens of cattle

Cited by 34 publications

References 21 publications

A genome-wide association study of immune response traits in Canadian Holstein cattle

A genome-wide association study of immune response traits in Canadian Holstein cattle

Comparison of innate immune agonists for induction of tracheal antimicrobial peptide gene expression in tracheal epithelial cells of cattle

The Immunology of Bovine Respiratory Disease

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