Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms

Abstract

In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain Paenarthrobacter nicotinovorans MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive Bacillus cereus and Gram-negative bacteria Pseudomonas aeruginosa. Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) analysis. The UV-Vis spectral analysis showed the absorption maxima at 466 nm which assured the synthesis of AgNPs. The FE-TEM analysis revealed the spherical shape of nanoparticles with the size range from 13 to 27 nm. The EDX and XRD analysis ensured the crystalline nature of biosynthesized AgNPs. The FTIR analysis revealed the involvement of different biomolecules for the synthesis of AgNPs as reducing and capping agents. The bacterial-mediated synthesized AgNPs inhibited the growth of pathogenic strains B. cereus and P. aeruginosa and developed a clear zone of inhibition (ZOI). The MIC and MBC for both pathogens were 12.5 µg/mL and 25 µg/mL, respectively. Moreover, field emission scanning electron microscopy analysis revealed that the synthesized AgNPs can destroy the outer membrane and alter the cell morphology of treated pathogens, leading to the death of cells. This study concludes the eco-friendly, facile and rapid synthesis of AgNPs using P. nicotinovorans MAHUQ-43 and synthesized AgNPs showed excellent antimicrobial activity against both Gram-positive and Gram-negative pathogens.

Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis

“…MAHUQ-52 mediated synthesized AgNPs against K. pneumoniae and S. Enteritidis were 17.6 ± 0.5 and 16.8 ± 0.9 mm, respectively. These findings are consistent with previous studies that showed the antimicrobial activities of biosynthesized AgNPs against Bacillus cereus and Pseudomonas aeruginosa [ 17 ]. Six standard antibiotic disks, including erythromycin, vancomycin, penicillin, novobiocin, oleandomycin and lincomycin, were also used as a control to check their antibacterial efficacy.…”

“…Bacterial strains of K. pneumoniae and S. Enteritidis (log-phase cells, approximately 1 × 10 7 CFU/mL) were each incubated with and without AgNPs (at MBC concentration) for 12 h at 37 °C. After the incubation period, samples were collected from both cultures and processed for FE-SEM analysis according to the previous description [ 17 ]. Briefly, the overnight-treated cells were centrifuged at 8000 rpm for 5 min, and the supernatant was poured out.…”

“…Therefore, it is essential to develop a simple, cost-effective, non-toxic, and environmentally friendly approach for the facile and mass production of metal NPs using biological systems. Biological synthesis can be performed using either bacteria [ 17 ], fungi [ 18 ] or plant extracts [ 19 , 20 ]. Among various biological entities, bacterial-mediated synthesis of NPs is mostly preferred due to their largescale production, high growth rate and ease of handling [ 13 , 21 ].…”

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

“…Viruses [27], bacteria and actinomycetes [28][29][30], yeasts and fungi [31][32][33], and algae and plants [34][35][36] have been applied for the synthesis of metallic nanoparticles. Due to the diverse set of bioactive constituents presented in plant extracts, the reduction of metal ions or metal oxides, followed by the nucleation and growth of nanoparticles, occurs in solution at room temperature [37][38][39].…”

Investigation on Green Synthesis, Biocompatibility, and Antibacterial Activity of Silver Nanoparticles Prepared Using Cistus incanus

“…Therefore, there is a fundamental need to develop clean, environmentally benign, non-toxic, and safe approaches to the preparation of nanomaterials exploiting sustainable and cost-effective technologies. These essential challenges have led researchers to place a focus on biological systems, including bacteria [ 7 ], fungi [ 8 ], algae [ 9 ], and plants [ 10 ]. Considering the cost-effectiveness of the preparation of nanoparticles (NPs), methods based on phytochemical-mediated reduction of metal ions may provide an economic and valuable alternative for the production of NPs [ 11 ].…”