Bacterial magnetosome and its potential application

Yan, Lei; Da, Huiyun; Zhang, Shuang; López, Viviana Morillo; Wang, Weidong

doi:10.1016/j.micres.2017.06.005

Cited by 83 publications

(64 citation statements)

References 140 publications

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“…Once absorbed, these ions can be readily removed by magnetic separation. [ 270 ] For example, an MTB strain UPB‐MAG05 has been found to be highly tolerant of Cd ions and can absorb these ions from Cd‐polluted waters. [ 271 ]…”

Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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Bacteria play an important role in the calcification process of natural minerals and within organisms ( Table 1). It is Microbe-mediated mineralization is ubiquitous in nature, involving bacteria, fungi, viruses, and algae. These mineralization processes comprise calcification, silicification, and iron mineralization. The mechanisms for mineral formation include extracellular and intracellular biomineralization. The mineral precipitating capability of microbes is often harnessed for green synthesis of metal nanoparticles, which are relatively less toxic compared with those synthesized through physical or chemical methods. Microbe-mediated mineralization has important applications ranging from pollutant removal and nonreactive carriers, to other industrial and biomedical applications. Herein, the different types of microbe-mediated biomineralization that occur in nature, their mechanisms, as well as their applications are elucidated to create a backdrop for future research.

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Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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“…The biological production of the biomineralized magnetosomes is strictly controlled at the gene level, and the magnetosomes are normally formed in different sizes and shapes in magnetosome membrane [6] . Magnetosome synthesis has recently been proposed as a model for the formation of prokaryotic organelles and biomineralization [8,9] . Although the details of the mechanism for the synthesis of magnetosomes are not exactly clear, studies have shown that the formation of magnets is a cellular process that depends on several stages, including the separation of the internal membrane of the cell, the transfer of ions, the crystallization of magnetite within these vesicles, and the formation and arrangement of adult crystals as a linear structure of the cellular skeleton [1,10] .…”

Section: Introuductionmentioning

confidence: 99%

Effects of Environmental Conditions on High-Yield Magnetosome Production by Magnetospirillum gryphiswaldense MSR-1

Jajan

Hosseini

Ghorbani

et al. 2019

ibj

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Background: Magnetotactic bacteria are a heterogeneous group of Gram-negative prokaryote cells that produce linear chains of magnetic particles called magnetosomes, intracellular organelles composed of magnetic iron particles. Many important applications have been defined for magnetic nanoparticles in biotechnology, such as cell separation applications, as well as acting as carriers of enzymes, antibodies, or anti-cancer drugs. Since the bacterial growth is difficult and the yield of magnetosome production is low, the application of magnetosome has not been developed on a commercial scale. Methods: Magnetospirillum gryphiswaldense strain MSR-1 was used in a modified current culture medium supplemented by different concentrations of oxygen, iron, carbon, and nitrogen, to increase the yield of magnetosomes. Results: Our improved MSR-1 culture medium increased magnetosome yield, magnetosome number per bacterial cell, magnetic response, and bacterial cell growth yield significantly. The yield of magnetosome increased approximately four times. The optimized culture medium containing 25 mM of Na-pyruvate, 40 mM of NaNO3, 200 µM of ferrous sulfate, and 5-10 ppm of dissolved oxygen (DO) resulted in 186.67 mg of magnetosome per liter of culture medium. The iron uptake increased significantly, and the magnetic response of the bacteria to the magnetic field was higher than threefold as compared to the previously reported procedures. Conclusion: This technique not only decreases the cultivation time but also reduces the production cost. In this modified method, the iron and DO are the major factors affecting the production of magnetosome by M. gryphiswaldense strain MSR-1. However, refining this technique will enable a further yield of magnetosome and cell density.

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“…When the ultrastructural details of BMs were first reported in 1980, it generated much interest . The distinctive characteristics of BMs suggested that they could be used as an effective vehicle, particularly as a potential magnetic carrier for drugs.…”

Section: Discussionmentioning

confidence: 99%

Preparation and anti‐tumor efficiency evaluation of bacterial magnetosome–anti‐4‐1BB antibody complex: Bacterial magnetosome as antibody carriers isolated from Magnetospirillum gryphiswaldense

Tang

Wang

Zhou

et al. 2019

Biotech and App Biochem

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Bacterial magnetosomes (BMs) are used as carriers for antibodies, enzymes, and nucleic acids. This study aimed to demonstrate the clinical utility of BMs derived from Magnetospirillum gryphiswaldense for use in anti-tumor immunotherapy. Bis(sulfosuccinimidyl) suberate (BS3) was used to prepare BM-anti-4-1BB antibody complexes. We used syngeneic TC-1 mouse models of cancer to investigate whether BMs combined with an anti-4-1BB agonistic antibodies could enhance the therapeutic effects of anti-4-1BB antibodies in localized disease settings. Anti-4-1BB antibodies were combined with purified BMs at a concentration of 168 mg antibody per milligram BM (mg IgG/mg BM) using BS3.The anti-4-1BB antibody-coupled BMs (BM-Ab complexes) and control BMs displayed similar morphologies and measurements when examined by transmission electron microscope (TEM). In a mouse tumor model, intravenous injection of BM-Abs combined with magnetic treatment resulted in greater tumor protection than did other treatment methods (P < 0.05). These results demonstrate the in vivo anti-tumor properties of BM-Abs complexes. The coupling of anti-4-1BB antibodies to magnetosomes may have potential for clinical application to antitumor antibody therapy. C 2019 small in size (40-120 nm) and are covered with a stable lipid membrane, which allows the magnetosomes to disperse very well [1]. The bilayer membrane component of BMs contains many amino groups, which can be combined with biological molecules that include enzymes, antibodies, and antitumor medicines [2,3]. Many in vitro results have demonstrated that BMs have many advantages over artificial iron oxide nanoparticles, particularly when the nanoparticles are used for biological applications [4][5][6].The cellular receptor 4-1BB (CD137) is a significant mediator of T-cell activation and persistence and exerts particularly strong effects on cytotoxic CD8+ T cells [7,8]. The administration of an anti-4-1BB antibody has been shown to eradicate small tumors when used alone or in combination with other antibodies in some mouse models [9,10].

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Bacterial magnetosome and its potential application

Cited by 83 publications

References 140 publications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Effects of Environmental Conditions on High-Yield Magnetosome Production by Magnetospirillum gryphiswaldense MSR-1

Preparation and anti‐tumor efficiency evaluation of bacterial magnetosome–anti‐4‐1BB antibody complex: Bacterial magnetosome as antibody carriers isolated from Magnetospirillum gryphiswaldense

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