Bacterial isolates from bryozoan Pleurocodonellina sp.: Diversity and antimicrobial potential against pathogenic bacteria

Abstract

Abstract. Asagabaldan MA, Bedoux G, Bourgougnon N, Kristiana R, Ayuningrum D, Sabdono A, Trianto A, Radjasa OK. 2019. Bacterial isolates from bryozoan Pleurocodonellina sp.: Diversity and antimicrobial potential against pathogenic bacteria. Biodiversitas 20: 2528-2535. There is an urgent need to discover new compounds with antibacterial activity, which can be developed into lead structures for the treatment of human disease caused by multidrug-resistant (MDR) bacteria. In this study, we focussed on bryozoan-associated bacteria to screen them toward antibacterial activities, since the microbiome of these organisms can still be regarded as under-investigated. Most of the few publications about bryozoan-associated bacteria focused on taxonomy and the potential as producers of antibacterial natural products were neglected. Four specimens of bryozoan Pleurocodonellina sp. were collected from Teluk Awur, Jepara in Java Sea, Indonesia. Therefrom, 56 bacterial strains were isolated, and 17 displayed antibacterial activities against MDR bacteria Pseudomonas aruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Enterobacter cloacae, and methicillin-resistant Staphylococcus aureus (MRSA). Taxonomic identification of the bacteria by 16S rRNA gene sequencing revealed them belonging to the genera Virgibacillus, Pseudoalteromonas, Halomonas, and Bacillus. Most interestingly, the genus Virgibacillus was dominantly obtained from the Pleurocodonellina sp. specimens, i.e., 12 active isolates. Nevertheless, the best activities against MDR bacteria (both Gram-positive and Gram-negative) were contributed to isolates showing >99% identity to Pseudoalteromonas. The results further suggest adding the genus Virgibacillus as bacteria associated with bryozoan, since to the best of our knowledge there were no reports of this genus isolated from bryozoan.

Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

“…Antibacterial activity of the isolates were determined by measuring the diameter of the inhibition zone in mm around the bacterial isolates. The levels of antibacterial activity were categorized as follows: no antibacterial activity (-), 0-1 mm (+), 1-3 mm (++), 3-7 mm (+++) and 7-15 mm (++++) (Asagabaldan et al 2019). The inhibition area was measured to confirm the antibacterial activity (Apsari et al 2019).…”

In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections

“…Thus, new antibacterial agents from natural biological sources are required. Biological antibacterial agents can be obtained from honey (Panjaitan et al 2018), mushrooms (Prastiyanto et al 2020b;, isolate bacteria from marine organisms (Asagabaldan et al 2019), bacteriocins (Lestari et al 2019), fruits (Prastiyanto et al 2020d;Wahyuni et al 2019), latex (Prastiyanto et al 2020c) and seeds (Ilvani et al 2019;Prastiyanto et al 2020a). Many studies in the medical field reported the importance of traditional medicinal plants as alternatives of antimicrobial agents (Akhtar and Mirza 2015;Aumeeruddy-elalfi et al 2015;Prastiyanto et al 2021).…”

Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus

“…Infections caused by MRSA bacteria consume more health care resources than are consumed by patients infected with non-resistant strains of the same bacteria (Word Health Organization 2018). In consequence, novel antibacterial agents are needed from natural biological sources (Asagabaldan et al 2019;Lestari et al 2019;Prastiyanto et al 2020b;2020a, 2020c. Neutral proteoglycans derived from the P. ostreatus mycelia have been reported that could be used as immunomodulators and anticancer agents (Sarangi et al 2006).…”