Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula)

Walcher, Petra; Cui, Xianlan; Arrow, Jane; Scobie, S.; Molinia, F. C.; Cowan, P. E.; Lubitz, Werner; Duckworth, Janine A.

doi:10.1016/j.vaccine.2008.09.088

Cited by 34 publications

(22 citation statements)

References 29 publications

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“…VLPs are compounds artificially constructed to resemble viruses that are non-infectious because they do not contain any viral genetic material (Cross et al 2011). Initial results of BGs as an oro-nasal delivery system for ZP-based vaccines in brushtail possums showed a significant reduction in egg-fertilisation rates (Walcher et al 2008) and offspring production (Duckworth in Cross et al 2011). GnRH-VLP also elicited antibodies to GnRH (Cross et al 2011).…”

Section: Delivery Methodsmentioning

confidence: 99%

Fertility control to mitigate human–wildlife conflicts: a review

2014

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Abstract. As human populations grow, conflicts with wildlife increase. Concurrently, concerns about the welfare, safety and environmental impacts of conventional lethal methods of wildlife management restrict the options available for conflict mitigation. In parallel, there is increasing interest in using fertility control to manage wildlife. The present review aimed at analysing trends in research on fertility control for wildlife, illustrating developments in fertility-control technologies and delivery methods of fertility-control agents, summarising the conclusions of empirical and theoretical studies of fertility control applied at the population level and offering criteria to guide decisions regarding the suitability of fertility control to mitigate human-wildlife conflicts. The review highlighted a growing interest in fertility control for wildlife, underpinned by increasing numbers of scientific studies. Most current practical applications of fertility control for wild mammals use injectable single-dose immunocontraceptive vaccines mainly aimed at sterilising females, although many of these vaccines are not yet commercially available. One oral avian contraceptive, nicarbazin, is commercially available in some countries. Potential new methods of remote contraceptive delivery include bacterial ghosts, virus-like particles and genetically modified transmissible and non-transmissible organisms, although none of these have yet progressed to field testing. In parallel, new species-specific delivery systems have been developed. The results of population-level studies of fertility control indicated that this approach may increase survival and affect social and spatial behaviour of treated animals, although the effects are species-and context-specific. The present studies suggested that a substantial initial effort is generally required to reduce population growth if fertility control is the sole wildlife management method. However, several empirical and field studies have demonstrated that fertility control, particularly of isolated populations, can be successfully used to limit population growth and reduce human-wildlife conflicts. In parallel, there is growing recognition of the possible synergy between fertility control and disease vaccination to optimise the maintenance of herd immunity in the management of wildlife diseases. The review provides a decision tree that can be used to determine whether fertility control should be employed to resolve specific human-wildlife conflicts. These criteria encompass public consultation, considerations about animal welfare and feasibility, evaluation of population responses, costs and sustainability.

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Section: Delivery Methodsmentioning

confidence: 99%

Fertility control to mitigate human–wildlife conflicts: a review

2014

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“…The ghost cassette comprises the phiX174 lysis E gene and the phage λpR37-cI857 regulatory system to generate the ghost cells. The cI857 protein turns off E gene expression under the control of the PR promoter at temperatures below 28 C, whereas the promoter turns the gene on at temperatures above 42 C (Mayr et al 2005a;Walcher et al 2008). The plasmid pMMP81 harbors the asd gene (Hur & Lee 2011b) and the ompA signal sequence; the genes encoding the fimbrial antigens were cloned into plasmid pMMP81 for expression of fimbrial antigens in the JOL1255 strain.…”

Section: Bacterial Strains Ghost Plasmids and Growth Conditionsmentioning

confidence: 99%

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Hur

Kim

et al. 2015

Veterinary Quarterly

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Results: All groups with single, double, and triple inoculations of the ghost showed higher humoral and mucosal immune responses than the control group. In particular, the groups with intramuscular double and triple inoculations showed significantly higher immune responses. In addition, oral inoculation with a combination of the ghost and MONTANIDE IMS 1113 (MI1113) resulted in high and prolonged induction of intestinal IgA levels. Conclusion: These results indicated that both systemic and mucosal immunity against ETEC fimbrial antigens expressed by the ghost are induced by intramuscular booster inoculation with the ghost, and that addition of M1113 to the ghost was found to result in prominent induction of mucosal immunity through oral inoculation.

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“…It is imperative to note that the process of Protein E-specific lysis does not result in physical or chemical denaturation of bacterial surface structures. The bacterial ghosts have been suggested to be a great alternative method for inactivated non-living whole-cell vaccination [ 76 ]. Depending on the site-directed sequences included in the fusion, a variety of foreign proteins can be expressed within or on the cell envelop of bacterial ghosts [ 77 ].…”

Section: Bacterial Adhesionmentioning

confidence: 99%